HIT1_YEAST
ID HIT1_YEAST Reviewed; 164 AA.
AC P46973; D6VWM6;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Protein HIT1;
GN Name=HIT1; OrderedLocusNames=YJR055W; ORFNames=J1705;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1325386; DOI=10.1093/genetics/131.4.821;
RA Kawakami K., Shafer B.K., Garfinkel D.J., Strathern J.N., Nakamura Y.;
RT "Ty element-induced temperature-sensitive mutations of Saccharomyces
RT cerevisiae.";
RL Genetics 131:821-832(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8840504;
RX DOI=10.1002/(sici)1097-0061(199607)12:9<869::aid-yea964>3.0.co;2-1;
RA Huang M.-E., Manus V., Chuat J.-C., Galibert F.;
RT "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading
RT frames and a gene cluster with a counterpart on chromosome XI.";
RL Yeast 12:869-875(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- INTERACTION:
CC P46973; Q08932: RSA1; NbExp=8; IntAct=EBI-8359, EBI-16186;
CC -!- MISCELLANEOUS: Present with 2730 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; D11103; BAA01878.1; -; Genomic_DNA.
DR EMBL; Z49555; CAA89583.1; -; Genomic_DNA.
DR EMBL; L47993; AAB39281.1; -; Genomic_DNA.
DR EMBL; AY558097; AAS56423.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08842.1; -; Genomic_DNA.
DR PIR; S57074; S57074.
DR RefSeq; NP_012589.1; NM_001181713.1.
DR PDB; 2MJF; NMR; -; B=70-164.
DR PDB; 2N95; NMR; -; A=1-46.
DR PDBsum; 2MJF; -.
DR PDBsum; 2N95; -.
DR AlphaFoldDB; P46973; -.
DR BMRB; P46973; -.
DR SMR; P46973; -.
DR BioGRID; 33810; 70.
DR DIP; DIP-4241N; -.
DR IntAct; P46973; 4.
DR STRING; 4932.YJR055W; -.
DR MaxQB; P46973; -.
DR PaxDb; P46973; -.
DR PRIDE; P46973; -.
DR EnsemblFungi; YJR055W_mRNA; YJR055W; YJR055W.
DR GeneID; 853516; -.
DR KEGG; sce:YJR055W; -.
DR SGD; S000003816; HIT1.
DR VEuPathDB; FungiDB:YJR055W; -.
DR eggNOG; ENOG502S27I; Eukaryota.
DR HOGENOM; CLU_1619339_0_0_1; -.
DR InParanoid; P46973; -.
DR OMA; QYNTVKF; -.
DR BioCyc; YEAST:G3O-31689-MON; -.
DR PRO; PR:P46973; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P46973; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0070761; C:pre-snoRNP complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000492; P:box C/D snoRNP assembly; IMP:SGD.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR GO; GO:0048254; P:snoRNA localization; IBA:GO_Central.
DR InterPro; IPR040722; Hit1_C.
DR InterPro; IPR007529; Znf_HIT.
DR InterPro; IPR040197; ZNHIT3.
DR PANTHER; PTHR13483:SF11; PTHR13483:SF11; 1.
DR Pfam; PF18268; Hit1_C; 1.
DR Pfam; PF04438; zf-HIT; 1.
DR PROSITE; PS51083; ZF_HIT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..164
FT /note="Protein HIT1"
FT /id="PRO_0000173556"
FT ZN_FING 8..49
FT /note="HIT-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 8
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 11
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 28
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT BINDING 32
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00453"
FT CONFLICT 36..37
FT /note="AA -> RR (in Ref. 1; BAA01878)"
FT /evidence="ECO:0000305"
FT TURN 9..11
FT /evidence="ECO:0007829|PDB:2N95"
FT STRAND 13..15
FT /evidence="ECO:0007829|PDB:2N95"
FT TURN 21..23
FT /evidence="ECO:0007829|PDB:2N95"
FT STRAND 26..29
FT /evidence="ECO:0007829|PDB:2N95"
FT HELIX 30..33
FT /evidence="ECO:0007829|PDB:2N95"
FT STRAND 36..39
FT /evidence="ECO:0007829|PDB:2N95"
FT HELIX 77..84
FT /evidence="ECO:0007829|PDB:2MJF"
FT HELIX 87..92
FT /evidence="ECO:0007829|PDB:2MJF"
FT HELIX 96..110
FT /evidence="ECO:0007829|PDB:2MJF"
FT STRAND 119..122
FT /evidence="ECO:0007829|PDB:2MJF"
FT HELIX 123..134
FT /evidence="ECO:0007829|PDB:2MJF"
FT TURN 135..137
FT /evidence="ECO:0007829|PDB:2MJF"
FT HELIX 146..160
FT /evidence="ECO:0007829|PDB:2MJF"
SQ SEQUENCE 164 AA; 18376 MW; EAC6ABB5457F87AA CRC64;
MVSSAVKCGI CRGVDGKYKC PKCGVRYCSL KCYKDAAKHV HKESEQPRAG TEANVEVVNN
DKIINSSLAM NKTLKTKAFD DIYQNSAELQ ELLKYNTVKF HLAKVYRILS STVNDGSSGK
MNSDLQKELA VNYLNTLRYG GIHYNEAIEE FCQILLDKLN AVKK
