HIT4_ARATH
ID HIT4_ARATH Reviewed; 434 AA.
AC A2RVJ8; Q9FIA6;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein HEAT INTOLERANT 4 {ECO:0000303|PubMed:23408827};
GN Name=HIT4 {ECO:0000303|PubMed:23408827};
GN OrderedLocusNames=At5g10010 {ECO:0000312|Araport:AT5G10010};
GN ORFNames=T31P16.7 {ECO:0000312|EMBL:BAB17025.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF SER-227, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=23408827; DOI=10.1093/jxb/ert030;
RA Wang L.C., Wu J.R., Chang W.L., Yeh C.H., Ke Y.T., Lu C.A., Wu S.J.;
RT "Arabidopsis HIT4 encodes a novel chromocentre-localized protein involved
RT in the heat reactivation of transcriptionally silent loci and is essential
RT for heat tolerance in plants.";
RL J. Exp. Bot. 64:1689-1701(2013).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF SER-227.
RC STRAIN=cv. Columbia;
RX PubMed=25329561; DOI=10.1111/nph.13088;
RA Wang L.-C., Wu J.-R., Hsu Y.-J., Wu S.-J.;
RT "Arabidopsis HIT4, a regulator involved in heat-triggered reorganization of
RT chromatin and release of transcriptional gene silencing, relocates from
RT chromocenters to the nucleolus in response to heat stress.";
RL New Phytol. 205:544-554(2015).
CC -!- FUNCTION: Essential protein required for basal thermotolerance,
CC especially during heat-induced chromocentre decondensation, thus
CC regulating transcriptional gene silencing (TGS).
CC {ECO:0000269|PubMed:23408827, ECO:0000269|PubMed:25329561}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:23408827, ECO:0000269|PubMed:25329561}. Nucleus,
CC nucleolus {ECO:0000269|PubMed:25329561}. Note=Localizes to the
CC chromocentre at 23 degrees Celsius, a condensed heterochromatin domain
CC that harbors repetitive elements for which transcription is normally
CC suppressed by transcriptional gene silencing (TGS) (PubMed:23408827,
CC PubMed:25329561). Relocates from the chromocenter to the nucleolus in
CC response to heat (30 hours at 37 degrees Celsius or 30 minutes at 44
CC degrees Celsius), before heat-induced decondensation of chromocenters
CC occurs (PubMed:25329561). {ECO:0000269|PubMed:23408827,
CC ECO:0000269|PubMed:25329561}.
CC -!- DEVELOPMENTAL STAGE: Expressed predominately at the early stages during
CC embryogenesis. {ECO:0000269|PubMed:23408827}.
CC -!- DISRUPTION PHENOTYPE: Embryo lethal in homozygous plants.
CC Hypersensitivity to heat leading to the inability to withstand
CC prolonged heat stress (4 days at 37 degrees Celsius), probably due to
CC impaired heat-induced chromocentre decondensation associated with
CC attenuated heat reactivation of various transcriptional gene silencing
CC (TGS) loci. {ECO:0000269|PubMed:23408827}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB17025.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB016893; BAB17025.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91481.1; -; Genomic_DNA.
DR EMBL; BT029989; ABN04727.1; -; mRNA.
DR RefSeq; NP_196563.2; NM_121039.5.
DR AlphaFoldDB; A2RVJ8; -.
DR STRING; 3702.AT5G10010.1; -.
DR iPTMnet; A2RVJ8; -.
DR MetOSite; A2RVJ8; -.
DR PaxDb; A2RVJ8; -.
DR PRIDE; A2RVJ8; -.
DR ProteomicsDB; 230337; -.
DR DNASU; 830863; -.
DR EnsemblPlants; AT5G10010.1; AT5G10010.1; AT5G10010.
DR GeneID; 830863; -.
DR Gramene; AT5G10010.1; AT5G10010.1; AT5G10010.
DR KEGG; ath:AT5G10010; -.
DR Araport; AT5G10010; -.
DR TAIR; locus:2184053; AT5G10010.
DR eggNOG; ENOG502QZIQ; Eukaryota.
DR HOGENOM; CLU_042584_2_1_1; -.
DR InParanoid; A2RVJ8; -.
DR OMA; GANKIVH; -.
DR OrthoDB; 973873at2759; -.
DR PhylomeDB; A2RVJ8; -.
DR PRO; PR:A2RVJ8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; A2RVJ8; baseline and differential.
DR GO; GO:0010369; C:chromocenter; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0010286; P:heat acclimation; IMP:TAIR.
DR GO; GO:0045814; P:negative regulation of gene expression, epigenetic; IMP:UniProtKB.
DR GO; GO:0031452; P:negative regulation of heterochromatin assembly; IMP:GO_Central.
DR GO; GO:1900034; P:regulation of cellular response to heat; IEA:InterPro.
DR GO; GO:0009408; P:response to heat; IDA:UniProtKB.
DR InterPro; IPR039313; HIT4.
DR PANTHER; PTHR33704; PTHR33704; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Stress response.
FT CHAIN 1..434
FT /note="Protein HEAT INTOLERANT 4"
FT /id="PRO_0000441704"
FT REGION 1..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 363..394
FT /evidence="ECO:0000255"
FT MOTIF 1..8
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 95..102
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 370..377
FT /note="Nuclear localization signal 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 22..57
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..86
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 109..131
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 227
FT /note="S->Y: In hit4-1; hypersensitivity to heat leading to
FT the inability to withstand prolonged heat stress (4 days at
FT 37 degrees Celsius). Reduced relocation from the
FT chromocenter to the nucleolus in response to heat resulting
FT in insufficient decompaction of chromocenters and impaired
FT reactivation of transcriptional gene silencing (TGS)."
FT /evidence="ECO:0000269|PubMed:23408827,
FT ECO:0000269|PubMed:25329561"
SQ SEQUENCE 434 AA; 50517 MW; 9C07B875EB1C9FC9 CRC64;
MKKGAKRKGV SKAGRKAAVA ETQNDEVIEE TTKTTQEESQ QHEEEVVDEV KENGEEEEAK
GDQEEEEDAK PDSLEEDEEN QEDEVKAEEV KEEVEKKPVA RRGGKRKRAT KKDTEIKDEK
KPVPKAKKPR AAKVKEEPVY FEEKRSLEDL WKVAFPVGTE WDQLDALYEF NWDFQNLEEA
LEEGGKLYGK KVYVFGCTEP QLVPYKGANK IVHVPAVVVI ESPFPPSDKI GITSVQREVE
EIIPMKKMKM DWLPYIPIEK RDRQVDKMNS QIFTLGCTQR RSALRHMKED QLKKFEYCLP
YFYQPFKEDE LEQSTEVQIM FPSEPPVVCE FDWEFDELQE FVDKLVEEEA LPAEQADEFK
EYVKEQVRAA KKANREAKDA RKKAIEEMSE DTKQAFQKMK FYKFYPQPSP DTPDVSGVQS
PFINRYYGKA HEVL
