ID   HIT_BACSU               Reviewed;         145 AA.
AC   O07513;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Protein hit;
GN   Name=hit; Synonyms=yhaE; OrderedLocusNames=BSU10030;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA   Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA   Venema G., Bron S.;
RT   "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT   subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT   many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL   Microbiology 144:859-875(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RG   New York structural genomix research consortium (NYSGXRC);
RT   "Crystal structure of a member of HIT family of proteins from Bacillus
RT   subtilis.";
RL   Submitted (FEB-2009) to the PDB data bank.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Y14077; CAA74410.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12843.1; -; Genomic_DNA.
DR   PIR; A69642; A69642.
DR   RefSeq; NP_388884.1; NC_000964.3.
DR   RefSeq; WP_003233231.1; NZ_JNCM01000035.1.
DR   PDB; 1Y23; X-ray; 2.30 A; A/B/C/D/E=1-145.
DR   PDBsum; 1Y23; -.
DR   AlphaFoldDB; O07513; -.
DR   SMR; O07513; -.
DR   STRING; 224308.BSU10030; -.
DR   jPOST; O07513; -.
DR   PaxDb; O07513; -.
DR   PRIDE; O07513; -.
DR   EnsemblBacteria; CAB12843; CAB12843; BSU_10030.
DR   GeneID; 939769; -.
DR   KEGG; bsu:BSU10030; -.
DR   PATRIC; fig|224308.179.peg.1079; -.
DR   eggNOG; COG0537; Bacteria.
DR   InParanoid; O07513; -.
DR   OMA; SDCLFCK; -.
DR   PhylomeDB; O07513; -.
DR   BioCyc; BSUB:BSU10030-MON; -.
DR   EvolutionaryTrace; O07513; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009117; P:nucleotide metabolic process; IBA:GO_Central.
DR   CDD; cd01277; HINT_subgroup; 1.
DR   Gene3D; 3.30.428.10; -; 1.
DR   InterPro; IPR039384; HINT.
DR   InterPro; IPR019808; Histidine_triad_CS.
DR   InterPro; IPR001310; Histidine_triad_HIT.
DR   InterPro; IPR011146; HIT-like.
DR   InterPro; IPR036265; HIT-like_sf.
DR   PANTHER; PTHR46648; PTHR46648; 1.
DR   Pfam; PF01230; HIT; 1.
DR   PRINTS; PR00332; HISTRIAD.
DR   SUPFAM; SSF54197; SSF54197; 1.
DR   PROSITE; PS00892; HIT_1; 1.
DR   PROSITE; PS51084; HIT_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..145
FT                   /note="Protein hit"
FT                   /id="PRO_0000109807"
FT   DOMAIN          8..116
FT                   /note="HIT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00464"
FT   MOTIF           100..104
FT                   /note="Histidine triad motif"
FT   HELIX           8..13
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   STRAND          21..24
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   STRAND          26..32
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   STRAND          42..48
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   HELIX           53..55
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   HELIX           58..62
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   TURN            63..66
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   HELIX           67..79
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   STRAND          82..91
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   HELIX           92..94
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   STRAND          98..100
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   STRAND          103..108
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   STRAND          114..119
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   HELIX           123..125
FT                   /evidence="ECO:0007829|PDB:1Y23"
FT   HELIX           128..141
FT                   /evidence="ECO:0007829|PDB:1Y23"
SQ   SEQUENCE   145 AA;  16298 MW;  5380143EE22AB336 CRC64;
     MHCAENCIFC KIIAGDIPSA KVYEDEHVLA FLDISQVTKG HTLVIPKTHI ENVYEFTDEL
     AKQYFHAVPK IARAIRDEFE PIGLNTLNNN GEKAGQSVFH YHMHIIPRYG KGDGFGAVWK
     THADDYKPED LQNISSSIAK RLASS