HIUH1_CAEEL
ID HIUH1_CAEEL Reviewed; 135 AA.
AC Q21882; Q1NZ16;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Probable 5-hydroxyisourate hydrolase R09H10.3;
DE Short=HIU hydrolase;
DE Short=HIUHase;
DE EC=3.5.2.17;
DE AltName: Full=Transthyretin-like protein R09H10.3;
DE Flags: Precursor;
GN ORFNames=R09H10.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-
CC 4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-
CC dihydro-1H-imidazole-5-carboxylate + H(+); Xref=Rhea:RHEA:23736,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18072,
CC ChEBI:CHEBI:58639; EC=3.5.2.17;
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- INTERACTION:
CC Q21882; G5ED33: eps-8; NbExp=3; IntAct=EBI-311857, EBI-2315916;
CC Q21882; Q21882: R09H10.3; NbExp=2; IntAct=EBI-311857, EBI-311857;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q21882-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q21882-2; Sequence=VSP_020154;
CC -!- MISCELLANEOUS: HIU hydrolysis also occurs spontaneously, but more
CC slowly.
CC -!- SIMILARITY: Belongs to the transthyretin family. 5-hydroxyisourate
CC hydrolase subfamily. {ECO:0000305}.
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DR EMBL; Z77134; CAB00872.2; -; Genomic_DNA.
DR EMBL; Z77134; CAJ90503.1; -; Genomic_DNA.
DR PIR; T24096; T24096.
DR RefSeq; NP_001040980.1; NM_001047515.4. [Q21882-1]
DR RefSeq; NP_001040981.1; NM_001047516.3. [Q21882-2]
DR AlphaFoldDB; Q21882; -.
DR SMR; Q21882; -.
DR BioGRID; 52440; 7.
DR IntAct; Q21882; 3.
DR STRING; 6239.R09H10.3a; -.
DR EPD; Q21882; -.
DR PaxDb; Q21882; -.
DR PeptideAtlas; Q21882; -.
DR EnsemblMetazoa; R09H10.3a.1; R09H10.3a.1; WBGene00011181. [Q21882-1]
DR EnsemblMetazoa; R09H10.3b.1; R09H10.3b.1; WBGene00011181. [Q21882-2]
DR GeneID; 187757; -.
DR KEGG; cel:CELE_R09H10.3; -.
DR UCSC; R09H10.3a.1; c. elegans. [Q21882-1]
DR CTD; 187757; -.
DR WormBase; R09H10.3a; CE34083; WBGene00011181; -. [Q21882-1]
DR WormBase; R09H10.3b; CE12662; WBGene00011181; -. [Q21882-2]
DR eggNOG; KOG3006; Eukaryota.
DR GeneTree; ENSGT00940000153229; -.
DR InParanoid; Q21882; -.
DR OMA; ENQNYHI; -.
DR OrthoDB; 1453185at2759; -.
DR PhylomeDB; Q21882; -.
DR Reactome; R-CEL-3000171; Non-integrin membrane-ECM interactions.
DR Reactome; R-CEL-6798695; Neutrophil degranulation.
DR Reactome; R-CEL-975634; Retinoid metabolism and transport.
DR PRO; PR:Q21882; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00011181; Expressed in larva and 4 other tissues.
DR GO; GO:0033971; F:hydroxyisourate hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006144; P:purine nucleobase metabolic process; IBA:GO_Central.
DR CDD; cd05822; TLP_HIUase; 1.
DR Gene3D; 2.60.40.180; -; 1.
DR InterPro; IPR014306; Hydroxyisourate_hydrolase.
DR InterPro; IPR023418; Thyroxine_BS.
DR InterPro; IPR000895; Transthyretin/HIU_hydrolase.
DR InterPro; IPR023416; Transthyretin/HIU_hydrolase_d.
DR InterPro; IPR036817; Transthyretin/HIU_hydrolase_sf.
DR InterPro; IPR023419; Transthyretin_CS.
DR PANTHER; PTHR10395; PTHR10395; 1.
DR Pfam; PF00576; Transthyretin; 1.
DR PRINTS; PR00189; TRNSTHYRETIN.
DR SMART; SM00095; TR_THY; 1.
DR SUPFAM; SSF49472; SSF49472; 1.
DR TIGRFAMs; TIGR02962; hdxy_isourate; 1.
DR PROSITE; PS00768; TRANSTHYRETIN_1; 1.
DR PROSITE; PS00769; TRANSTHYRETIN_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Purine metabolism; Reference proteome;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..135
FT /note="Probable 5-hydroxyisourate hydrolase R09H10.3"
FT /id="PRO_0000050603"
FT BINDING 30
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 68
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 132
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..14
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_020154"
SQ SEQUENCE 135 AA; 15129 MW; 5D5AC6D752D94843 CRC64;
MNKFSLFFAL TATLMTITES AVPTASISAH VLDISGGSPA GGVQILAYIQ QNDDWTKIGS
EFTQDNGRVD WVSPDFTLIP GTYRLVYITE PYYKAKNVES FYPYVEVVFN IRDATQHYHV
PLTLSPWGYS TYRGS
