HJC_ARCFU
ID HJC_ARCFU Reviewed; 136 AA.
AC O28314;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Holliday junction resolvase Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000255|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000255|HAMAP-Rule:MF_01490}; OrderedLocusNames=AF_1965;
OS Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS 100126 / VC-16).
OC Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC Archaeoglobus.
OX NCBI_TaxID=224325;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX PubMed=9389475; DOI=10.1038/37052;
RA Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA Smith H.O., Woese C.R., Venter J.C.;
RT "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT archaeon Archaeoglobus fulgidus.";
RL Nature 390:364-370(1997).
RN [2]
RP PRELIMINARY CRYSTALLIZATION.
RX PubMed=16511128; DOI=10.1107/s1744309105018269;
RA Biertumpfel C., Basquin J., Birkenbihl R.P., Suck D., Sauter C.;
RT "Characterization of crystals of the Hjc resolvase from Archaeoglobus
RT fulgidus grown in gel by counter-diffusion.";
RL Acta Crystallogr. F 61:684-687(2005).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS) OF 2-136.
RA Carolis C., Koehler C., Sauter C., Basquin J., Suck D., Toeroe I.;
RT "1.6 A resolution structure of Archaeoglobus fulgidus Hjc, a Holliday
RT junction resolvase from an archaeal hyperthermophile.";
RL Submitted (MAR-2009) to the PDB data bank.
RN [4]
RP X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 2-136.
RA Carolis C., Koehler C., Sauter C., Basquin J., Suck D., Toeroe I.;
RT "Crystal structures of Holliday junction resolvases from Archaeoglobus
RT fulgidus bound to DNA substrate.";
RL Submitted (MAY-2009) to the PDB data bank.
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated
CC to produce recombinant products. {ECO:0000255|HAMAP-Rule:MF_01490}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01490};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01490}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000255|HAMAP-Rule:MF_01490}.
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DR EMBL; AE000782; AAB89293.1; -; Genomic_DNA.
DR PIR; D69495; D69495.
DR RefSeq; WP_010879457.1; NC_000917.1.
DR PDB; 2WCW; X-ray; 1.58 A; A/B/C/D=2-136.
DR PDB; 2WCZ; X-ray; 1.65 A; A/B=2-136.
DR PDB; 2WIW; X-ray; 1.80 A; A/B=2-136.
DR PDB; 2WIZ; X-ray; 3.30 A; A/B=2-136.
DR PDB; 2WJ0; X-ray; 3.10 A; A/B=2-136.
DR PDBsum; 2WCW; -.
DR PDBsum; 2WCZ; -.
DR PDBsum; 2WIW; -.
DR PDBsum; 2WIZ; -.
DR PDBsum; 2WJ0; -.
DR AlphaFoldDB; O28314; -.
DR SMR; O28314; -.
DR STRING; 224325.AF_1965; -.
DR EnsemblBacteria; AAB89293; AAB89293; AF_1965.
DR GeneID; 24795708; -.
DR KEGG; afu:AF_1965; -.
DR eggNOG; arCOG00919; Archaea.
DR HOGENOM; CLU_139546_0_0_2; -.
DR OMA; WYFFHPG; -.
DR OrthoDB; 97278at2157; -.
DR PhylomeDB; O28314; -.
DR EvolutionaryTrace; O28314; -.
DR Proteomes; UP000002199; Chromosome.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; archeal_HJR; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR39651; PTHR39651; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome.
FT CHAIN 1..136
FT /note="Holliday junction resolvase Hjc"
FT /id="PRO_0000429153"
FT ACT_SITE 29
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 9
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 38
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 51
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255"
FT HELIX 5..18
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 22..26
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 29..31
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:2WCZ"
FT STRAND 38..42
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 47..54
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 60..63
FT /evidence="ECO:0007829|PDB:2WCW"
FT HELIX 64..77
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 80..86
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 93..96
FT /evidence="ECO:0007829|PDB:2WCW"
FT HELIX 97..99
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:2WCW"
FT STRAND 104..109
FT /evidence="ECO:0007829|PDB:2WCW"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:2WCW"
FT HELIX 114..116
FT /evidence="ECO:0007829|PDB:2WCW"
FT HELIX 120..125
FT /evidence="ECO:0007829|PDB:2WCW"
FT HELIX 126..128
FT /evidence="ECO:0007829|PDB:2WCW"
SQ SEQUENCE 136 AA; 15437 MW; C59D1FF6ABDE1AC8 CRC64;
MKSKGTRFER DLLVELWKAG FAAIRVAGSG VSPFPCPDIV AGNGRTYLAI EVKMRKELPL
YLSADEVEQL VTFARGFGAE AYVALKLPRK KWRFFPVQML ERTEKNFKID ESVYPLGLEI
AEVAGKFFQE RFGEKV
