HJC_HALVD
ID HJC_HALVD Reviewed; 140 AA.
AC D4GZB4; Q6H8N3;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Holliday junction resolvase Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000255|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000255|HAMAP-Rule:MF_01490}; OrderedLocusNames=HVO_0170;
GN ORFNames=C498_19104;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DS2 / DS70;
RA Haldenby S.T., Guy C.P., Bolt E.L., Allers T.;
RT "RadB, an archaeal Rad51 paralogue, is required for DNA repair in Haloferax
RT volcanii.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=DS2 / DS70;
RX PubMed=20667794; DOI=10.1016/j.dnarep.2010.06.012;
RA Lestini R., Duan Z., Allers T.;
RT "The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction
RT resolvase Hjc define alternative pathways that are essential for cell
RT viability in Haloferax volcanii.";
RL DNA Repair 9:994-1002(2010).
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated
CC to produce recombinant products (By similarity). Has a role that
CC partially overlaps that of Hef endonuclease; double deletions cannot be
CC constructed. In the presence of site-directed mutations that (probably)
CC abolish Hef helicase or nuclease activity, a hjc deletion cannot be
CC constructed. {ECO:0000255|HAMAP-Rule:MF_01490,
CC ECO:0000269|PubMed:20667794}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01490};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01490}.
CC -!- DISRUPTION PHENOTYPE: No alteration in sensitivity to UV light, slow
CC growth on mitomycin C. No effect on homologous recombination; double
CC hef/hjc deletion mutants cannot be constructed (synthetic lethality),
CC suggesting the enzymes play overlapping roles.
CC {ECO:0000269|PubMed:20667794}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000255|HAMAP-Rule:MF_01490}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ748744; CAG38617.1; -; Genomic_DNA.
DR EMBL; CP001956; ADE03775.1; -; Genomic_DNA.
DR EMBL; AOHU01000106; ELY23862.1; -; Genomic_DNA.
DR RefSeq; WP_004045284.1; NZ_AOHU01000106.1.
DR AlphaFoldDB; D4GZB4; -.
DR SMR; D4GZB4; -.
DR STRING; 309800.C498_19104; -.
DR PRIDE; D4GZB4; -.
DR EnsemblBacteria; ADE03775; ADE03775; HVO_0170.
DR EnsemblBacteria; ELY23862; ELY23862; C498_19104.
DR GeneID; 8926173; -.
DR KEGG; hvo:HVO_0170; -.
DR PATRIC; fig|309800.29.peg.3721; -.
DR eggNOG; arCOG00919; Archaea.
DR HOGENOM; CLU_139546_0_0_2; -.
DR OMA; WYFFHPG; -.
DR OrthoDB; 97278at2157; -.
DR Proteomes; UP000008243; Chromosome.
DR Proteomes; UP000011532; Unassembled WGS sequence.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; archeal_HJR; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR39651; PTHR39651; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Endonuclease;
KW Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..140
FT /note="Holliday junction resolvase Hjc"
FT /id="PRO_0000429154"
FT ACT_SITE 31
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 11
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 41
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 54
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT SITE 56
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255"
SQ SEQUENCE 140 AA; 15370 MW; 744BF520B4E0A6F9 CRC64;
MSSNRKGDRR ERELVNALDE AGFAVMRAPA SGSATTRELP DVLAGNGEVF YAIEAKASSG
RPIYLSGEEV EALVYFSRNF GAKARIAVRF DREDWYFFHP GDLYVTDGGN YRVKKETALA
EGEDFESFTG GPTQTKLGGD
