HJC_METTH
ID HJC_METTH Reviewed; 136 AA.
AC O27336;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Holliday junction resolvase Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000255|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000255|HAMAP-Rule:MF_01490}; OrderedLocusNames=MTH_1270;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES,
RP SUBUNIT, EXPRESSION IN E.COLI, AND DNA-BINDING.
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=11439025; DOI=10.1006/jmbi.2001.4791;
RA Bolt E.L., Lloyd R.G., Sharples G.J.;
RT "Genetic analysis of an archaeal Holliday junction resolvase in Escherichia
RT coli.";
RL J. Mol. Biol. 310:577-589(2001).
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated
CC to produce recombinant products. Cleaves both mobile and immobile
CC junctions. Converts a synthetic Hj to nicked duplex products. Binds a
CC variety of branched DNA structures but linear dsDNA poorly; cleaves
CC only 4-way junctions. Confers UV and mitomycin C resistance to E.coli
CC cells lacking the endogenous Hj machinery RuvABC. {ECO:0000255|HAMAP-
CC Rule:MF_01490, ECO:0000269|PubMed:11439025}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01490, ECO:0000269|PubMed:11439025};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01490,
CC ECO:0000269|PubMed:11439025};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01490,
CC ECO:0000269|PubMed:11439025};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 7.8-9.5. {ECO:0000269|PubMed:11439025};
CC Temperature dependence:
CC Optimum temperature is 55 degrees Celsius.
CC {ECO:0000269|PubMed:11439025};
CC -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:11439025}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000255|HAMAP-Rule:MF_01490}.
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DR EMBL; AE000666; AAB85757.1; -; Genomic_DNA.
DR PIR; F69036; F69036.
DR RefSeq; WP_010876892.1; NC_000916.1.
DR AlphaFoldDB; O27336; -.
DR SMR; O27336; -.
DR STRING; 187420.MTH_1270; -.
DR EnsemblBacteria; AAB85757; AAB85757; MTH_1270.
DR GeneID; 1471678; -.
DR KEGG; mth:MTH_1270; -.
DR PATRIC; fig|187420.15.peg.1247; -.
DR HOGENOM; CLU_139546_0_0_2; -.
DR OMA; WYFFHPG; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; archeal_HJR; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR39651; PTHR39651; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Endonuclease;
KW Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..136
FT /note="Holliday junction resolvase Hjc"
FT /id="PRO_0000429155"
FT ACT_SITE 29
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 9
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 39
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 52
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT SITE 54
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255"
SQ SEQUENCE 136 AA; 15396 MW; FE5D18497D97CFC3 CRC64;
MVKNGTRGER DLVKLLWEKG FAAMRAPASG GATKKPLPDI IAGNGEIYLA IEVKTTARER
IYIDSEKIGA LLRFSDIFGA RPYIGIKFRY RDWIFLSPGD LELTPSSNYR LDLDIALERG
RDLDEVTGNH RQTRLR
