ID   HJC_SULIR               Reviewed;         143 AA.
AC   F0NID3;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Holliday junction resolvase Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE            Short=Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE            EC=3.1.21.10 {ECO:0000255|HAMAP-Rule:MF_01490};
GN   Name=hjc {ECO:0000255|HAMAP-Rule:MF_01490}; OrderedLocusNames=SiRe_1431;
OS   Sulfolobus islandicus (strain REY15A).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfolobus.
OX   NCBI_TaxID=930945;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=REY15A;
RX   PubMed=21278296; DOI=10.1128/jb.01487-10;
RA   Guo L., Brugger K., Liu C., Shah S.A., Zheng H., Zhu Y., Wang S.,
RA   Lillestol R.K., Chen L., Frank J., Prangishvili D., Paulin L., She Q.,
RA   Huang L., Garrett R.A.;
RT   "Genome analyses of icelandic strains of Sulfolobus islandicus, model
RT   organisms for genetic and virus-host interaction studies.";
RL   J. Bacteriol. 193:1672-1680(2011).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=REY15A / E233S;
RX   PubMed=22062475; DOI=10.1016/j.dnarep.2011.10.009;
RA   Hong Y., Chu M., Li Y., Ni J., Sheng D., Hou G., She Q., Shen Y.;
RT   "Dissection of the functional domains of an archaeal Holliday junction
RT   helicase.";
RL   DNA Repair 11:102-111(2012).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=REY15A;
RX   PubMed=23356319; DOI=10.1042/bst20120285;
RA   Zhang C., Tian B., Li S., Ao X., Dalgaard K., Gokce S., Liang Y., She Q.;
RT   "Genetic manipulation in Sulfolobus islandicus and functional analysis of
RT   DNA repair genes.";
RL   Biochem. Soc. Trans. 41:405-410(2013).
CC   -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC       junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC       DNA junctions introducing paired nicks in opposing strands, leaving a
CC       5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated
CC       to produce recombinant products. {ECO:0000255|HAMAP-Rule:MF_01490}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC         single-stranded crossover between two homologous DNA duplexes
CC         (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01490};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01490};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01490};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01490}.
CC   -!- DISRUPTION PHENOTYPE: Not essential, it can be disrupted.
CC       {ECO:0000269|PubMed:22062475, ECO:0000269|PubMed:23356319}.
CC   -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC       {ECO:0000255|HAMAP-Rule:MF_01490}.
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DR   EMBL; CP002425; ADX85497.1; -; Genomic_DNA.
DR   RefSeq; WP_012711550.1; NC_017276.1.
DR   AlphaFoldDB; F0NID3; -.
DR   SMR; F0NID3; -.
DR   STRING; 930945.SiRe_1431; -.
DR   EnsemblBacteria; ADX85497; ADX85497; SiRe_1431.
DR   GeneID; 7814663; -.
DR   GeneID; 7940907; -.
DR   GeneID; 8761593; -.
DR   KEGG; sir:SiRe_1431; -.
DR   eggNOG; arCOG00919; Archaea.
DR   HOGENOM; CLU_139546_1_0_2; -.
DR   OMA; WYFFHPG; -.
DR   BRENDA; 3.1.21.10; 8240.
DR   Proteomes; UP000002664; Chromosome.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   CDD; cd00523; archeal_HJR; 1.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR   InterPro; IPR002732; Hjc.
DR   InterPro; IPR014428; Hjc_arc.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR39651; PTHR39651; 1.
DR   Pfam; PF01870; Hjc; 1.
DR   PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding; Endonuclease;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease.
FT   CHAIN           1..143
FT                   /note="Holliday junction resolvase Hjc"
FT                   /id="PRO_0000429157"
FT   ACT_SITE        32
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT   BINDING         42
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT   BINDING         55
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT   SITE            57
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   143 AA;  15916 MW;  98DF8FE8474B5CAC CRC64;
     MNAKKRKGSA VERNIVGKLR DKGFAVVRAP ASGSKRKDPI PDIIALKSGV IILIEMKSRK
     DKEGKIYVRR EQAEGIMEFA RKSGGTLFLG VKKPGVLKFI PFDKLRRTET GNYVADSEIE
     GLDLEDLVRL VEAKVSKTLD NFL