HJC_SULTO
ID HJC_SULTO Reviewed; 144 AA.
AC F9VND5;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=Holliday junction resolvase Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000255|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000255|HAMAP-Rule:MF_01490}; OrderedLocusNames=STK_14440;
OS Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
OS (Sulfolobus tokodaii).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfurisphaera.
OX NCBI_TaxID=273063;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=11572479; DOI=10.1093/dnares/8.4.123;
RA Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M.,
RA Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y.,
RA Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T.,
RA Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K.,
RA Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.;
RT "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon,
RT Sulfolobus tokodaii strain7.";
RL DNA Res. 8:123-140(2001).
RN [2]
RP ACTIVITY REGULATION.
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=18782564; DOI=10.1016/j.bbrc.2008.08.150;
RA Lu S., Li Z., Wang Z., Ma X., Sheng D., Ni J., Shen Y.;
RT "Spatial subunit distribution and in vitro functions of the novel trimeric
RT PCNA complex from Sulfolobus tokodaii.";
RL Biochem. Biophys. Res. Commun. 376:369-374(2008).
RN [3]
RP FUNCTION AS AN ENDONUCLEASE, INTERACTION WITH HEL308, AND SUBUNIT.
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=18296528; DOI=10.1128/jb.01662-07;
RA Li Z., Lu S., Hou G., Ma X., Sheng D., Ni J., Shen Y.;
RT "Hjm/Hel308A DNA helicase from Sulfolobus tokodaii promotes replication
RT fork regression and interacts with Hjc endonuclease in vitro.";
RL J. Bacteriol. 190:3006-3017(2008).
RN [4]
RP INTERACTION WITH HJM, AND DNA-BINDING.
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=22062475; DOI=10.1016/j.dnarep.2011.10.009;
RA Hong Y., Chu M., Li Y., Ni J., Sheng D., Hou G., She Q., Shen Y.;
RT "Dissection of the functional domains of an archaeal Holliday junction
RT helicase.";
RL DNA Repair 11:102-111(2012).
RN [5]
RP ACTIVITY REGULATION, INTERACTION WITH RADC2, AND SUBUNIT.
RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX PubMed=22437993; DOI=10.1007/s11427-012-4292-0;
RA Wang L., Sheng D., Han W., Huang B., Zhu S., Ni J., Li J., Shen Y.;
RT "Sulfolobus tokodaii RadA paralog, stRadC2, is involved in DNA
RT recombination via interaction with RadA and Hjc.";
RL Sci. China Life Sci. 55:261-267(2012).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 2-144.
RA Sarai N., Kagawa W., Kurumizaka H., Yokoyama S.;
RT "Crystal structure of Holliday junction resolvase ST1444.";
RL Submitted (MAR-2007) to the PDB data bank.
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated
CC to produce recombinant products. Inhibits the helicase activity of
CC Hel308 (Hjm). {ECO:0000255|HAMAP-Rule:MF_01490,
CC ECO:0000269|PubMed:18296528}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01490};
CC -!- ACTIVITY REGULATION: Cleavage stimulated by PCNA123 and PCNA323
CC (PubMed:18782564) and by RadC2 (PubMed:22437993).
CC {ECO:0000269|PubMed:18782564, ECO:0000269|PubMed:22437993}.
CC -!- SUBUNIT: Homodimer; forms a 2:1 complex with Hel308 (Hjm). May form a
CC complex with Holliday junction DNA, Hjc and Hjm. {ECO:0000255|HAMAP-
CC Rule:MF_01490, ECO:0000269|PubMed:18296528}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000255|HAMAP-Rule:MF_01490}.
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DR EMBL; BA000023; BAK54581.1; -; Genomic_DNA.
DR RefSeq; WP_052846967.1; NC_003106.2.
DR PDB; 2EO0; X-ray; 2.40 A; A/B=2-144.
DR PDBsum; 2EO0; -.
DR AlphaFoldDB; F9VND5; -.
DR SMR; F9VND5; -.
DR STRING; 273063.STK_14440; -.
DR EnsemblBacteria; BAK54581; BAK54581; STK_14440.
DR GeneID; 1459477; -.
DR KEGG; sto:STK_14440; -.
DR PATRIC; fig|273063.9.peg.1646; -.
DR eggNOG; arCOG00919; Archaea.
DR OMA; WYFFHPG; -.
DR OrthoDB; 97278at2157; -.
DR Proteomes; UP000001015; Chromosome.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; archeal_HJR; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR39651; PTHR39651; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome.
FT CHAIN 1..144
FT /note="Holliday junction resolvase Hjc"
FT /id="PRO_0000429160"
FT ACT_SITE 32
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 12
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 42
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 55
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT SITE 57
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255"
FT HELIX 10..21
FT /evidence="ECO:0007829|PDB:2EO0"
FT STRAND 25..27
FT /evidence="ECO:0007829|PDB:2EO0"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:2EO0"
FT STRAND 42..47
FT /evidence="ECO:0007829|PDB:2EO0"
FT STRAND 50..58
FT /evidence="ECO:0007829|PDB:2EO0"
FT STRAND 65..67
FT /evidence="ECO:0007829|PDB:2EO0"
FT HELIX 69..82
FT /evidence="ECO:0007829|PDB:2EO0"
FT STRAND 85..92
FT /evidence="ECO:0007829|PDB:2EO0"
FT STRAND 95..100
FT /evidence="ECO:0007829|PDB:2EO0"
FT HELIX 101..103
FT /evidence="ECO:0007829|PDB:2EO0"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:2EO0"
FT HELIX 116..120
FT /evidence="ECO:0007829|PDB:2EO0"
FT HELIX 125..140
FT /evidence="ECO:0007829|PDB:2EO0"
SQ SEQUENCE 144 AA; 16336 MW; C85536716D9FD36A CRC64;
MNSNKSRGSS VERYIVSRLR DKGFAVIRAP ASGSKRKDHV PDIIALKSGV IILIEVKSRK
NGQKIYIEKE QAEGIREFAK RSGGELFLGV KLPKMLRFIK FDMLRQTEGG NYAIDLETVE
KGMELEDLVR YVESKISRTL DSFL
