HJC_THEKO
ID HJC_THEKO Reviewed; 128 AA.
AC Q5JGD9;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Holliday junction resolvase Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000255|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000255|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000255|HAMAP-Rule:MF_01490}; OrderedLocusNames=TK1175;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
RN [2]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=21178304; DOI=10.1266/ggs.85.243;
RA Fujikane R., Ishino S., Ishino Y., Forterre P.;
RT "Genetic analysis of DNA repair in the hyperthermophilic archaeon,
RT Thermococcus kodakaraensis.";
RL Genes Genet. Syst. 85:243-257(2010).
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated
CC to produce recombinant products. {ECO:0000255|HAMAP-Rule:MF_01490}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01490};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01490}.
CC -!- DISRUPTION PHENOTYPE: Not essential, it can be disrupted. Cell survival
CC is unaffected by several DNA damaging agents.
CC {ECO:0000269|PubMed:21178304}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000255|HAMAP-Rule:MF_01490}.
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DR EMBL; AP006878; BAD85364.1; -; Genomic_DNA.
DR RefSeq; WP_011250126.1; NC_006624.1.
DR AlphaFoldDB; Q5JGD9; -.
DR SMR; Q5JGD9; -.
DR STRING; 69014.TK1175; -.
DR EnsemblBacteria; BAD85364; BAD85364; TK1175.
DR GeneID; 3235203; -.
DR KEGG; tko:TK1175; -.
DR PATRIC; fig|69014.16.peg.1150; -.
DR eggNOG; arCOG00919; Archaea.
DR HOGENOM; CLU_139546_2_0_2; -.
DR InParanoid; Q5JGD9; -.
DR OMA; WYFFHPG; -.
DR OrthoDB; 97278at2157; -.
DR PhylomeDB; Q5JGD9; -.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd00523; archeal_HJR; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR39651; PTHR39651; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Endonuclease;
KW Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..128
FT /note="Holliday junction resolvase Hjc"
FT /id="PRO_0000429161"
FT ACT_SITE 30
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 10
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 34
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT BINDING 47
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01490"
FT SITE 49
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255"
SQ SEQUENCE 128 AA; 14473 MW; 5249A3247BC9715B CRC64;
MRYRRGASAE RELVKLLESK GFAVLRSAGS HKIDLVAGNG KEYLCIEVKS TRSRKLYLPI
EDVEKLVEFA GRFGGRPVLA VKFVNVGWRF YGPNRLEHGE KSYKIDLETP FMTLDGLLGK
QRTLEGVL
