ID   HK2_MYCTU               Reviewed;         156 AA.
AC   O07777; L0T462;
DT   09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Sensor histidine kinase component HK2;
DE            EC=2.7.13.3;
GN   OrderedLocusNames=Rv0601c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, AND PHOSPHORYLATION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=17434492; DOI=10.1016/j.febslet.2007.03.089;
RA   Shrivastava R., Ghosh A.K., Das A.K.;
RT   "Probing the nucleotide binding and phosphorylation by the histidine kinase
RT   of a novel three-protein two-component system from Mycobacterium
RT   tuberculosis.";
RL   FEBS Lett. 581:1903-1909(2007).
RN   [3]
RP   SUBUNIT, AND INTERACTION WITH HK1 AND TCRA.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=19246748; DOI=10.1099/mic.0.019059-0;
RA   Shrivastava R., Ghosh A.K., Das A.K.;
RT   "Intra- and intermolecular domain interactions among novel two-component
RT   system proteins coded by Rv0600c, Rv0601c and Rv0602c of Mycobacterium
RT   tuberculosis.";
RL   Microbiology 155:772-779(2009).
CC   -!- FUNCTION: Member of the three-protein two-component system
CC       HK1/HK2/TcrA. HK2 transfers its phosphoryl group to TcrA.
CC       {ECO:0000269|PubMed:17434492}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBUNIT: Homodimer. Each monomer interacts with HK1 and the receiver
CC       domain of TcrA. {ECO:0000269|PubMed:19246748}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- PTM: Phosphorylated by HK1. {ECO:0000269|PubMed:17434492}.
CC   -!- MISCELLANEOUS: HK1 and HK2 are merged into a single protein with a
CC       distinct N-terminal sequence in strain CDC 1551 / Oshkosh.
CC   -!- CAUTION: HK1 and HK2 are incomplete as individual proteins but can
CC       complement each other's function. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43340.1; -; Genomic_DNA.
DR   PIR; D70909; D70909.
DR   RefSeq; NP_215115.1; NC_000962.3.
DR   RefSeq; WP_003403144.1; NC_018143.2.
DR   AlphaFoldDB; O07777; -.
DR   SMR; O07777; -.
DR   IntAct; O07777; 1.
DR   MINT; O07777; -.
DR   STRING; 83332.Rv0601c; -.
DR   PaxDb; O07777; -.
DR   GeneID; 887868; -.
DR   KEGG; mtu:Rv0601c; -.
DR   PATRIC; fig|83332.111.peg.664; -.
DR   TubercuList; Rv0601c; -.
DR   eggNOG; COG2972; Bacteria.
DR   InParanoid; O07777; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IDA:MTBBASE.
DR   GO; GO:0042803; F:protein homodimerization activity; IPI:MTBBASE.
DR   GO; GO:0004672; F:protein kinase activity; IDA:MTBBASE.
DR   GO; GO:0070298; P:negative regulation of phosphorelay signal transduction system; IDA:MTBBASE.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IBA:GO_Central.
DR   CDD; cd00082; HisKA; 1.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   Pfam; PF00672; HAMP; 1.
DR   SMART; SM00304; HAMP; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   PROSITE; PS50885; HAMP; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Kinase; Membrane; Phosphoprotein; Reference proteome;
KW   Transferase; Transmembrane; Transmembrane helix;
KW   Two-component regulatory system.
FT   CHAIN           1..156
FT                   /note="Sensor histidine kinase component HK2"
FT                   /id="PRO_0000391080"
FT   TOPO_DOM        1..42
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        64..156
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          64..120
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          128..156
FT                   /note="Histidine kinase; first part"
FT   MOD_RES         131
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   156 AA;  16595 MW;  B014C305809C60B2 CRC64;
     MALVLAAAGA VTVVQFRDAA HEADPDGALR GLTDDITADL VRELVTILPI VLVIAAVAAY
     LLSRAALRPV DRIRAAAQTL TTTPHPDTDA PLPVPPTDDE IAWLATTLNT MLTRLQRALA
     HEQQFVADAS HELRTPLALL TTELELRCAG PDPPTS