HKT11_ORYSJ
ID HKT11_ORYSJ Reviewed; 552 AA.
AC Q7XPF8; C7J0U7; Q8L542;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Cation transporter HKT1;1 {ECO:0000303|PubMed:16809061};
DE Short=OsHKT1;1 {ECO:0000303|PubMed:16809061};
DE AltName: Full=Cation transporter HKT4 {ECO:0000303|PubMed:12795699};
DE Short=OsHKT4 {ECO:0000303|PubMed:12795699};
GN Name=HKT1;1 {ECO:0000303|PubMed:16809061};
GN Synonyms=HKT4 {ECO:0000303|PubMed:16809061};
GN OrderedLocusNames=Os04g0607500 {ECO:0000312|EMBL:BAS90908.1},
GN LOC_Os04g51820 {ECO:0000305};
GN ORFNames=OSJNBa0060N03.3 {ECO:0000312|EMBL:CAE03638.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, BIOPHYSICOCHEMICAL
RP PROPERTIES, TISSUE SPECIFICITY, INDUCTION, AND NOMENCLATURE.
RC STRAIN=cv. Nipponbare;
RX PubMed=12795699; DOI=10.1046/j.1365-313x.2003.01764.x;
RA Garciadeblas B., Senn M.E., Banuelos M.A., Rodriguez-Navarro A.;
RT "Sodium transport and HKT transporters: the rice model.";
RL Plant J. 34:788-801(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16809061; DOI=10.1016/j.tplants.2006.06.001;
RA Platten J.D., Cotsaftis O., Berthomieu P., Bohnert H., Davenport R.J.,
RA Fairbairn D.J., Horie T., Leigh R.A., Lin H.X., Luan S., Maeser P.,
RA Pantoja O., Rodriguez-Navarro A., Schachtman D.P., Schroeder J.I.,
RA Sentenac H., Uozumi N., Very A.A., Zhu J.K., Dennis E.S., Tester M.;
RT "Nomenclature for HKT transporters, key determinants of plant salinity
RT tolerance.";
RL Trends Plant Sci. 11:372-374(2006).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19482918; DOI=10.1104/pp.109.138008;
RA Jabnoune M., Espeout S., Mieulet D., Fizames C., Verdeil J.L., Conejero G.,
RA Rodriguez-Navarro A., Sentenac H., Guiderdoni E., Abdelly C., Very A.A.;
RT "Diversity in expression patterns and functional properties in the rice HKT
RT transporter family.";
RL Plant Physiol. 150:1955-1971(2009).
CC -!- FUNCTION: Functions as a low-affinity sodium transporter.
CC {ECO:0000269|PubMed:12795699, ECO:0000269|PubMed:19482918}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Na(+)(in) = Na(+)(out); Xref=Rhea:RHEA:34963,
CC ChEBI:CHEBI:29101; Evidence={ECO:0000269|PubMed:12795699,
CC ECO:0000269|PubMed:19482918};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=11 mM for Na(+) {ECO:0000269|PubMed:12795699};
CC Vmax=33 nmol/min/mg enzyme with sodium as ion
CC {ECO:0000269|PubMed:12795699};
CC Note=Measured in yeast strain disrupted for potassium transporters
CC TRK1 and TRK2.;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in shoots (PubMed:12795699). In roots,
CC expressed in epidermis, exodermis, cortex, and sieve elements and
CC companion cells of phloem (PubMed:19482918). In mature leaves,
CC expressed in large highly vacuolated cells of the adaxial epidermis,
CC phloem and xylem (PubMed:19482918). {ECO:0000269|PubMed:12795699,
CC ECO:0000269|PubMed:19482918}.
CC -!- INDUCTION: By potassium starvation in roots.
CC {ECO:0000269|PubMed:12795699}.
CC -!- DOMAIN: HKT transporters are proposed to contain 4 pore-forming regions
CC enclosed by transmembrane segments with each containing a potassium
CC channel-like selectivity filter motif. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TrkH potassium transport family. HKT (TC
CC 2.A.38.3) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE03638.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ491815; CAD37182.1; -; Genomic_DNA.
DR EMBL; AJ491816; CAD37183.1; -; mRNA.
DR EMBL; AL606691; CAE03638.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008210; BAH92812.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS90908.1; -; Genomic_DNA.
DR RefSeq; XP_015633650.1; XM_015778164.1.
DR AlphaFoldDB; Q7XPF8; -.
DR STRING; 4530.OS04T0607500-01; -.
DR TCDB; 2.A.38.3.5; the k(+) transporter (trk) family.
DR PaxDb; Q7XPF8; -.
DR PRIDE; Q7XPF8; -.
DR EnsemblPlants; Os04t0607500-01; Os04t0607500-01; Os04g0607500.
DR GeneID; 9266695; -.
DR Gramene; Os04t0607500-01; Os04t0607500-01; Os04g0607500.
DR KEGG; osa:9266695; -.
DR eggNOG; KOG1341; Eukaryota.
DR HOGENOM; CLU_008384_2_0_1; -.
DR InParanoid; Q7XPF8; -.
DR OMA; FTFLACI; -.
DR OrthoDB; 385384at2759; -.
DR SABIO-RK; Q7XPF8; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR ExpressionAtlas; Q7XPF8; baseline and differential.
DR Genevisible; Q7XPF8; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProt.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR003445; Cat_transpt.
DR Pfam; PF02386; TrkH; 1.
PE 1: Evidence at protein level;
KW Ion transport; Membrane; Reference proteome; Sodium; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..552
FT /note="Cation transporter HKT1;1"
FT /id="PRO_0000070469"
FT TOPO_DOM 1..70
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 423..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..471
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..552
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 552 AA; 61862 MW; 215F4870A594258C CRC64;
MHPPSLVLDT LKRIKLYIAM KLLLPNSEVP RIYWEKAQHL CGFLSMKLIS RARCVASSVK
QSYSFLVCKS NPLVVQLVYF VIISFAGFLA LKNLKPQGKP GPKDLDLLFT SVSTLTVSSM
ATVEMEDLSD RQLWVLILLM LMGGEVFTSM LGLYFNNANA NRNENSQRSL PSISLDIEFN
SPANNGDHKI TECGQSEETM SQNQVQQNKS ITYNPCAVLV RIVTGYFVAT VISSSVIIII
YFWIDSDARN VLKSKEINMY TFCIFTAVSS FANCGFTPLN SNMQPFRKNW VLLLLVIPQI
LAGNTLFSPL LRLCVWVLGK VSGKAEYAYI LQHPGETGYK HLHVRRNSVY IVLSVTGLIL
LQVMFICSFE WNSESLEGMN WLQKLVGLLF QSVNTRQAGE SILDISTLSP STLLLFAVVM
YLPSDASFLT ANADNQPLTD KKTNSISRAL WRNFTVNKLS CLAMFTFLAC ITERKSISSD
PLNFNIFSIV FEIISAFGNV GYSLGYSCQK LLKPDATCKD ASYGFVGRWT EEGKLIVILV
MFLGRLKEFI LK
