HKT13_ORYSJ
ID HKT13_ORYSJ Reviewed; 531 AA.
AC Q6H501; Q0E3H2; Q8L4V1;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-MAY-2016, sequence version 2.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Cation transporter HKT1;3 {ECO:0000303|PubMed:16809061};
DE Short=OsHKT1;3 {ECO:0000303|PubMed:16809061};
DE AltName: Full=Cation transporter HKT6 {ECO:0000303|PubMed:12795699};
DE Short=OsHKT6 {ECO:0000303|PubMed:12795699};
GN Name=HKT1;3 {ECO:0000303|PubMed:16809061};
GN Synonyms=HKT6 {ECO:0000303|PubMed:12795699};
GN OrderedLocusNames=Os02g0175000 {ECO:0000312|EMBL:BAS77240.1},
GN LOC_Os02g07830 {ECO:0000305};
GN ORFNames=OSJNBa0073A21.31 {ECO:0000312|EMBL:BAD26198.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Nipponbare; TISSUE=Root;
RX PubMed=12795699; DOI=10.1046/j.1365-313x.2003.01764.x;
RA Garciadeblas B., Senn M.E., Banuelos M.A., Rodriguez-Navarro A.;
RT "Sodium transport and HKT transporters: the rice model.";
RL Plant J. 34:788-801(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16809061; DOI=10.1016/j.tplants.2006.06.001;
RA Platten J.D., Cotsaftis O., Berthomieu P., Bohnert H., Davenport R.J.,
RA Fairbairn D.J., Horie T., Leigh R.A., Lin H.X., Luan S., Maeser P.,
RA Pantoja O., Rodriguez-Navarro A., Schachtman D.P., Schroeder J.I.,
RA Sentenac H., Uozumi N., Very A.A., Zhu J.K., Dennis E.S., Tester M.;
RT "Nomenclature for HKT transporters, key determinants of plant salinity
RT tolerance.";
RL Trends Plant Sci. 11:372-374(2006).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19482918; DOI=10.1104/pp.109.138008;
RA Jabnoune M., Espeout S., Mieulet D., Fizames C., Verdeil J.L., Conejero G.,
RA Rodriguez-Navarro A., Sentenac H., Guiderdoni E., Abdelly C., Very A.A.;
RT "Diversity in expression patterns and functional properties in the rice HKT
RT transporter family.";
RL Plant Physiol. 150:1955-1971(2009).
RN [7]
RP FUNCTION, INTERACTION WITH CNIH1, AND SUBCELLULAR LOCATION.
RX PubMed=25750424; DOI=10.1093/jxb/erv069;
RA Rosas-Santiago P., Lagunas-Gomez D., Barkla B.J., Vera-Estrella R.,
RA Lalonde S., Jones A., Frommer W.B., Zimmermannova O., Sychrova H.,
RA Pantoja O.;
RT "Identification of rice cornichon as a possible cargo receptor for the
RT Golgi-localized sodium transporter OsHKT1;3.";
RL J. Exp. Bot. 66:2733-2748(2015).
CC -!- FUNCTION: Functions as a highly-selective sodium transporter
CC (PubMed:19482918, PubMed:25750424). Does not seem to function as
CC sodium-potassium cotransporter (PubMed:19482918, PubMed:25750424). May
CC be involved in turgor changes for rolling and unrolling of leaves in
CC response to environmental variations (PubMed:19482918).
CC {ECO:0000269|PubMed:19482918, ECO:0000269|PubMed:25750424}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Na(+)(in) = Na(+)(out); Xref=Rhea:RHEA:34963,
CC ChEBI:CHEBI:29101; Evidence={ECO:0000269|PubMed:19482918,
CC ECO:0000269|PubMed:25750424};
CC -!- SUBUNIT: Interacts with CNIH1. {ECO:0000269|PubMed:25750424}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:25750424}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000269|PubMed:25750424};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Weakly expressed (PubMed:12795699). In roots,
CC expressed in epidermis, exodermis, cortex, and sieve elements and
CC companion cells of phloem (PubMed:19482918). In mature leaves,
CC expressed in large highly vacuolated cells of the adaxial epidermis,
CC phloem and xylem (PubMed:19482918). {ECO:0000269|PubMed:12795699,
CC ECO:0000269|PubMed:19482918}.
CC -!- DOMAIN: HKT transporters are proposed to contain 4 pore-forming regions
CC enclosed by transmembrane segments with each containing a potassium
CC channel-like selectivity filter motif. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TrkH potassium transport family. HKT (TC
CC 2.A.38.3) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD26198.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD27606.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ491817; CAD37184.1; -; Genomic_DNA.
DR EMBL; AJ491818; CAD37185.1; -; mRNA.
DR EMBL; AP003992; BAD27606.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005772; BAD26198.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008208; BAF07966.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS77240.1; -; Genomic_DNA.
DR RefSeq; XP_015626193.1; XM_015770707.1.
DR AlphaFoldDB; Q6H501; -.
DR STRING; 4530.OS02T0175000-01; -.
DR PaxDb; Q6H501; -.
DR PRIDE; Q6H501; -.
DR EnsemblPlants; Os02t0175000-01; Os02t0175000-01; Os02g0175000.
DR GeneID; 4328468; -.
DR Gramene; Os02t0175000-01; Os02t0175000-01; Os02g0175000.
DR KEGG; osa:4328468; -.
DR eggNOG; KOG1341; Eukaryota.
DR InParanoid; Q6H501; -.
DR OMA; MFEISSA; -.
DR OrthoDB; 385384at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6H501; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProt.
DR InterPro; IPR003445; Cat_transpt.
DR Pfam; PF02386; TrkH; 2.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Golgi apparatus; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..530
FT /note="Cation transporter HKT1;3"
FT /id="PRO_0000070470"
FT TOPO_DOM 1..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..321
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 404..421
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..514
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 515..530
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 531 AA; 59305 MW; 3DF02E1C6B513C38 CRC64;
MNHCLVVSHK KLQTFRTFAA SKFSSFTKSA QKSIKYSFQF IYQNNPLFVH VAYFALISFA
GYGSLKVLKP RDKSNTLKDL DVLFTSVSAS TVSSMATVEM EDFSSAQLWV LTILMLIGGE
VFTSMLGIHF MRAEFGTKES VSTRDHSPCI DIESITSTKF GPSTQGTKVT VSFSELRMEN
GGHVEPKTIK FLGFVVMGYL LITNLGGSLL IYLYLNLVPS AHKILKRKGI GIIVFSVFTA
ISSVGNCGFT PVNENMIIFQ KNSILLLLIL PQILAGNTLF APCLRLMVWS LEKITGKKDC
RYILEYPKAI GYKHLMSTRE SVYLTLTVVS LIILQTVLFL SLEWSSVALD GMSNYQKIVS
ALFQSVNARH AGESVTDLSN LSSAILVLYT IMMYLPGYTS FLPRHDGEDS KTEKINKRKG
LLENWIFSHM SYLAIFVMLI CITERDSMAT DPLNFNVFSI LFEVVSAYGN VGFSVGYSCK
RLLNHDARCK DASYGFAGKW SDNGKAILII VMLFGRLKTF NMKGGRAWKL R
