HKT15_ORYSI
ID HKT15_ORYSI Reviewed; 554 AA.
AC A2WNZ9; A3FFK6; B8A6S2; Q3YAF4; Q8LQB2;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 44.
DE RecName: Full=Cation transporter HKT1;5 {ECO:0000303|PubMed:16809061};
DE Short=OsHKT1;5 {ECO:0000303|PubMed:16809061};
DE AltName: Full=Cation transporter HKT8 {ECO:0000303|PubMed:12795699};
DE Short=OsHKT8 {ECO:0000303|PubMed:12795699};
GN Name=HKT1;5 {ECO:0000303|PubMed:16809061};
GN Synonyms=HKT8 {ECO:0000303|PubMed:12795699},
GN SKC1 {ECO:0000303|PubMed:16155566};
GN ORFNames=OsI_01579 {ECO:0000312|EMBL:EEC70498.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION, AND VARIANTS HIS-184; ASP-332 AND VAL-395.
RC STRAIN=cv. Nona Bokra;
RX PubMed=16155566; DOI=10.1038/ng1643;
RA Ren Z.-H., Gao J.-P., Li L.-G., Cai X.-L., Huang W., Chao D.-Y., Zhu M.-Z.,
RA Wang Z.-Y., Luan S., Lin H.-X.;
RT "A rice quantitative trait locus for salt tolerance encodes a sodium
RT transporter.";
RL Nat. Genet. 37:1141-1146(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=20924028; DOI=10.1093/mp/ssq056;
RA Cotsaftis O., Plett D., Johnson A.A., Walia H., Wilson C., Ismail A.M.,
RA Close T.J., Tester M., Baumann U.;
RT "Root-specific transcript profiling of contrasting rice genotypes in
RT response to salinity stress.";
RL Mol. Plant 4:25-41(2011).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS HIS-184; ASP-332 AND VAL-395.
RC STRAIN=cv. Pokkali; TISSUE=Seedling;
RA Cotsaftis O., Tester M.;
RT "Mining for HKT allelic variability in rice.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Cho C., Kim K.H., Ahn E.-K., Park H., Choi M.-S., Chun J., Seo M.-S.,
RA Jin M., Kim D.-Y.;
RT "Selection of salt-tolerant silage rice through in vitro screening and
RT saltol QTL analysis.";
RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NOMENCLATURE.
RX PubMed=12795699; DOI=10.1046/j.1365-313x.2003.01764.x;
RA Garciadeblas B., Senn M.E., Banuelos M.A., Rodriguez-Navarro A.;
RT "Sodium transport and HKT transporters: the rice model.";
RL Plant J. 34:788-801(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16809061; DOI=10.1016/j.tplants.2006.06.001;
RA Platten J.D., Cotsaftis O., Berthomieu P., Bohnert H., Davenport R.J.,
RA Fairbairn D.J., Horie T., Leigh R.A., Lin H.X., Luan S., Maeser P.,
RA Pantoja O., Rodriguez-Navarro A., Schachtman D.P., Schroeder J.I.,
RA Sentenac H., Uozumi N., Very A.A., Zhu J.K., Dennis E.S., Tester M.;
RT "Nomenclature for HKT transporters, key determinants of plant salinity
RT tolerance.";
RL Trends Plant Sci. 11:372-374(2006).
CC -!- FUNCTION: Sodium transporter that under salt stress mediates sodium
CC exclusion in the phloem to prevent sodium transfer to young leaf blades
CC and reproductive tissues (By similarity). Contributes to salt-tolerance
CC in cultivars indica Nona Bokra and Pokkali (PubMed:16155566).
CC {ECO:0000250|UniProtKB:Q0JNB6, ECO:0000269|PubMed:16155566}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Na(+)(in) = Na(+)(out); Xref=Rhea:RHEA:34963,
CC ChEBI:CHEBI:29101; Evidence={ECO:0000250|UniProtKB:Q0JNB6};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305|PubMed:16155566}; Multi-
CC pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in parenchyma cells bordering
CC xylem vessels of nodes, internodes, leaf sheath bases, roots and
CC leaves. Expressed in phloem of leaf sheath bases.
CC {ECO:0000269|PubMed:16155566}.
CC -!- INDUCTION: By salt stress in roots. {ECO:0000269|PubMed:16155566}.
CC -!- DOMAIN: HKT transporters are proposed to contain 4 pore-forming regions
CC enclosed by transmembrane segments with each containing a potassium
CC channel-like selectivity filter motif. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TrkH potassium transport family. HKT (TC
CC 2.A.38.3) subfamily. {ECO:0000305}.
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DR EMBL; DQ148410; AAZ76552.1; -; mRNA.
DR EMBL; HQ162137; ADM87303.1; -; mRNA.
DR EMBL; EF373553; ABN48306.1; -; mRNA.
DR EMBL; MT495789; QXE97983.1; -; Genomic_DNA.
DR EMBL; CM000126; EEC70498.1; -; Genomic_DNA.
DR AlphaFoldDB; A2WNZ9; -.
DR EnsemblPlants; BGIOSGA001800-TA; BGIOSGA001800-PA; BGIOSGA001800.
DR Gramene; BGIOSGA001800-TA; BGIOSGA001800-PA; BGIOSGA001800.
DR Proteomes; UP000007015; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProt.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR003445; Cat_transpt.
DR Pfam; PF02386; TrkH; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Membrane; Reference proteome; Sodium; Sodium transport;
KW Stress response; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..554
FT /note="Cation transporter HKT1;5"
FT /id="PRO_0000303671"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..230
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 301..341
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 518..538
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 539..554
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 184
FT /note="R -> H (in strain: cv. Nona Bokra and cv. Pokkali;
FT higher sodium transport activity)"
FT /evidence="ECO:0000269|PubMed:16155566, ECO:0000269|Ref.3"
FT VARIANT 332
FT /note="H -> D (in strain: cv. Nona Bokra and cv. Pokkali;
FT higher sodium transport activity)"
FT /evidence="ECO:0000269|PubMed:16155566, ECO:0000269|Ref.3"
FT VARIANT 395
FT /note="L -> V (in strain: cv. Nona Bokra and cv. Pokkali;
FT higher sodium transport activity)"
FT /evidence="ECO:0000269|PubMed:16155566, ECO:0000269|Ref.3"
FT CONFLICT 128
FT /note="D -> N (in Ref. 2; ADM87303, 4; QXE97983 and 5;
FT EEC70498)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 554 AA; 60192 MW; A22370FFD6FA21C0 CRC64;
MSSLDATTPR YDEFKRIYHL FLFHAHPFWL QLLYFLFISL LGFLMLKALP MKTSMVPRPM
DLDLIFTSVS ATTVSSMVAV EMESFSNSQL LLITLLMLLG GEVFTSILGL YFTNAKYSSK
MIATLPDDDD HGGSGKPPPA TTSPSSTLVE LELAPPMDVV VVNPTTTATT HDEVELGLGR
RNKRGCTCTT THTSSSSSAS KTTTTRLLMF VVMGYHAVVH VAGYTAIVVY LSAVGGAGAV
VAGKGISAHT FAIFTVVSTF ANCGFVPTNE GMVSFRSFPG LLLLVMPHVL LGNTLFPVFL
RLAIAALERV TGWPELGELL IRRRRGGGEG YHHLLPSSRT RFLALTVAVL VVAQLALFCA
MEWGSDGLRG LTAGQKLVGA LFMAVNSRHS GEMVLDLSTV SSAVVVLYVV MMYLPPYTTF
VPVQDKHQQT GAQSGQEGSS SSSIWQKLLM SPLSCLAIFI VVICITERRQ IADDPINYSV
LNIVVEVISA YGNVGFSTGY SCARQVRPDG SCRDLWVGFS GKWSKQGKLT LMAVMFYGRL
KKFSLHGGQA WKIE
