HKT24_ORYSJ
ID HKT24_ORYSJ Reviewed; 509 AA.
AC Q8L4K5; Q0D9S4;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Cation transporter HKT2;4 {ECO:0000303|PubMed:16809061};
DE Short=OsHKT2;4 {ECO:0000303|PubMed:16809061};
DE AltName: Full=Cation transporter HKT9 {ECO:0000303|PubMed:12795699};
DE Short=OsHKT9 {ECO:0000303|PubMed:12795699};
GN Name=HKT2;4 {ECO:0000303|PubMed:16809061};
GN Synonyms=HKT9 {ECO:0000303|PubMed:12795699};
GN OrderedLocusNames=Os06g0701600 {ECO:0000312|EMBL:BAS99351.1},
GN LOC_Os06g48800 {ECO:0000305};
GN ORFNames=OsJ_22537 {ECO:0000312|EMBL:EEE66306.1},
GN P0596H10.8 {ECO:0000312|EMBL:BAD53774.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND NOMENCLATURE.
RC STRAIN=cv. Nipponbare;
RX PubMed=12795699; DOI=10.1046/j.1365-313x.2003.01764.x;
RA Garciadeblas B., Senn M.E., Banuelos M.A., Rodriguez-Navarro A.;
RT "Sodium transport and HKT transporters: the rice model.";
RL Plant J. 34:788-801(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16809061; DOI=10.1016/j.tplants.2006.06.001;
RA Platten J.D., Cotsaftis O., Berthomieu P., Bohnert H., Davenport R.J.,
RA Fairbairn D.J., Horie T., Leigh R.A., Lin H.X., Luan S., Maeser P.,
RA Pantoja O., Rodriguez-Navarro A., Schachtman D.P., Schroeder J.I.,
RA Sentenac H., Uozumi N., Very A.A., Zhu J.K., Dennis E.S., Tester M.;
RT "Nomenclature for HKT transporters, key determinants of plant salinity
RT tolerance.";
RL Trends Plant Sci. 11:372-374(2006).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=20351263; DOI=10.1073/pnas.1000698107;
RA Lan W.Z., Wang W., Wang S.M., Li L.G., Buchanan B.B., Lin H.X., Gao J.P.,
RA Luan S.;
RT "A rice high-affinity potassium transporter (HKT) conceals a calcium-
RT permeable cation channel.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:7089-7094(2010).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21610181; DOI=10.1104/pp.110.168047;
RA Horie T., Brodsky D.E., Costa A., Kaneko T., Lo Schiavo F., Katsuhara M.,
RA Schroeder J.I.;
RT "K+ transport by the OsHKT2;4 transporter from rice with atypical Na+
RT transport properties and competition in permeation of K+ over Mg2+ and Ca2+
RT ions.";
RL Plant Physiol. 156:1493-1507(2011).
CC -!- FUNCTION: High-affinity potassium transporter that does not show
CC potassium-sodium cotransport (PubMed:20351263, PubMed:21610181).
CC Potassium transport seems to be independent of sodium
CC (PubMed:21610181). Mediates transport of the divalent cations magnesium
CC and calcium in the absence of competing potassium ions
CC (PubMed:20351263, PubMed:21610181). Selectivity for potassium is
CC dominant over divalent cations, and magnesium and calcium transport may
CC be small and may depend on competing potassium concentrations
CC (PubMed:20351263, PubMed:21610181). {ECO:0000269|PubMed:20351263,
CC ECO:0000269|PubMed:21610181}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=K(+)(in) = K(+)(out); Xref=Rhea:RHEA:29463, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000269|PubMed:20351263, ECO:0000269|PubMed:21610181};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Mg(2+)(in) = Mg(2+)(out); Xref=Rhea:RHEA:29827,
CC ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:20351263,
CC ECO:0000269|PubMed:21610181};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Ca(2+)(in) = Ca(2+)(out); Xref=Rhea:RHEA:29671,
CC ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:20351263,
CC ECO:0000269|PubMed:21610181};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20351263,
CC ECO:0000269|PubMed:21610181}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Localizes to the plasma membrane.
CC {ECO:0000269|PubMed:20351263, ECO:0000269|PubMed:21610181}.
CC -!- TISSUE SPECIFICITY: Expressed in spikelets, leaf blades, leaf sheaths,
CC internodes, nodes, the base of stems and roots.
CC {ECO:0000269|PubMed:20351263}.
CC -!- DOMAIN: HKT transporters are proposed to contain 4 pore-forming regions
CC enclosed by transmembrane segments with each containing a potassium
CC channel-like selectivity filter motif. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TrkH potassium transport family. HKT (TC
CC 2.A.38.3) subfamily. {ECO:0000305}.
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DR EMBL; AJ491854; CAD37198.1; -; Genomic_DNA.
DR EMBL; AJ491855; CAD37199.1; -; mRNA.
DR EMBL; AP003726; BAD53774.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF20399.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS99351.1; -; Genomic_DNA.
DR EMBL; CM000143; EEE66306.1; -; Genomic_DNA.
DR RefSeq; XP_015641899.1; XM_015786413.1.
DR AlphaFoldDB; Q8L4K5; -.
DR SMR; Q8L4K5; -.
DR STRING; 4530.OS06T0701600-00; -.
DR PaxDb; Q8L4K5; -.
DR PRIDE; Q8L4K5; -.
DR EnsemblPlants; Os06t0701600-01; Os06t0701600-01; Os06g0701600.
DR GeneID; 4341970; -.
DR Gramene; Os06t0701600-01; Os06t0701600-01; Os06g0701600.
DR KEGG; osa:4341970; -.
DR eggNOG; KOG1341; Eukaryota.
DR HOGENOM; CLU_008384_2_0_1; -.
DR InParanoid; Q8L4K5; -.
DR OMA; ACHDKPY; -.
DR OrthoDB; 385384at2759; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR Genevisible; Q8L4K5; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProt.
DR InterPro; IPR003445; Cat_transpt.
DR Pfam; PF02386; TrkH; 2.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..509
FT /note="Cation transporter HKT2;4"
FT /id="PRO_0000070473"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 117..164
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..296
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 497..509
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 509 AA; 56117 MW; 706B84020F6B2BA1 CRC64;
MPIRLHIFVS SARHAINSSA LICRFIAFHL SPLLIHLSYF LIIDVLGFVA LVVLRPSNHK
YNPRYIDMFF LSTSAVTVTG LATTQMEDLS SSQIAVLTLL MFLGSEMFLS FLGLVLESSK
QNKHDPENRR VSSVTVCEQS HLEEAIPQTP SMNSTDIKRS CHKYLVFVVL AYMIIILVTG
SLLVFMYIAH VSSARDVLTR KSINKALFSI SVTVSSFTNG GLLPTNESMA VFSSNNGLLL
LLIGQILAGS TLLPMFLRLV IWALRGLRLA KAEEPDFMMN NSSSVGFSHL LPNLQTIFLA
AVEVAFVGMT VILFCCLNWD SAVFAGLTSL QKITNALFMA VSARQAGENS IDCSLVAPAA
LVLFMVMMYT PSLTKLFSAC QDHKQIGPES DDRTSKGKPF LKTMAFSPLA FNTTVIMLVC
ITERRSISTD PLNFSTFNII FEVISAYGNI GLSTGYSCSR QLQHQDGIAC HEKPYSFSGW
WSEPGKLILV LAMLYGRLNS KDSTSARTR
