ID   HL4_CATRO               Reviewed;         314 AA.
AC   A0A2P1GIY1;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   23-MAY-2018, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Hydrolase 4 {ECO:0000303|PubMed:29511102};
DE            Short=CrHL4 {ECO:0000303|PubMed:29511102};
DE            EC=4.-.-.- {ECO:0000269|PubMed:30256480};
GN   Name=HL4 {ECO:0000303|PubMed:29511102};
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=29511102; DOI=10.1073/pnas.1719979115;
RA   Qu Y., Easson M.E.A.M., Simionescu R., Hajicek J., Thamm A.M.K., Salim V.,
RA   De Luca V.;
RT   "Solution of the multistep pathway for assembly of corynanthean, strychnos,
RT   iboga, and aspidosperma monoterpenoid indole alkaloids from 19E-
RT   geissoschizine.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:3180-3185(2018).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC   STRAIN=cv. Little Delicata;
RX   PubMed=30256480; DOI=10.1111/tpj.14111;
RA   Qu Y., Safonova O., De Luca V.;
RT   "Completion of the canonical pathway for assembly of anticancer drugs
RT   vincristine/vinblastine in Catharanthus roseus.";
RL   Plant J. 97:257-266(2019).
CC   -!- FUNCTION: Component of the seco-iridoid and derivatives monoterpenoid
CC       indole alkaloids (MIAs, e.g. vincadifformine) biosynthesis pathway.
CC       Catalyzes the conversion of O-acetylstemmadenine (OAS) to
CC       vincadifformine. May also trigger the formation of additional unknown
CC       MIAs. {ECO:0000269|PubMed:30256480}.
CC   -!- PATHWAY: Alkaloid biosynthesis. {ECO:0000269|PubMed:30256480}.
CC   -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; MF770515; AVM85923.1; -; mRNA.
DR   AlphaFoldDB; A0A2P1GIY1; -.
DR   SMR; A0A2P1GIY1; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   Pfam; PF07859; Abhydrolase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Alkaloid metabolism; Lyase.
FT   CHAIN           1..314
FT                   /note="Hydrolase 4"
FT                   /id="PRO_0000446425"
FT   MOTIF           73..75
FT                   /note="Involved in the stabilization of the negatively
FT                   charged intermediate by the formation of the oxyanion hole"
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        165
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
FT   ACT_SITE        260
FT                   /evidence="ECO:0000250|UniProtKB:Q5NUF3"
SQ   SEQUENCE   314 AA;  35650 MW;  49ACD02E3981616D CRC64;
     MASSDETIFD LPPYIRSRKT PFFPLCFPSL NDPETGVSWK DVPISSQVSV RIYLPKITDH
     EKGEKLPILV YVHGAGFCLE SAFKSFFHTY VKHFVTEAKA IVVSVEFRLA PEHHLPSAYE
     DCWEALQWVA SHVGLDTSSL KTSIDKDPWI INYGDFERLY LWGDSTGANI VHNILIRAGK
     EKLKGGVKIL GAILYYPYFL IRTSSKQSDY MENEYRSYWK LAYPNAPGGN DNLMINPTAE
     NGPDLRGYGC SRLLVSMVAD EARDITLLYI DALERSGWKG ELDVADFEGD YFEIFSPDTE
     LGKNKLRRST SFIR