HLB1_ARATH
ID HLB1_ARATH Reviewed; 565 AA.
AC Q9FHY8;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 141.
DE RecName: Full=Protein HLB1 {ECO:0000303|PubMed:26941089};
DE AltName: Full=HYPERSENSITIVE TO LATRUNCULIN B1 {ECO:0000303|PubMed:26941089};
GN Name=HLB1 {ECO:0000303|PubMed:26941089};
GN OrderedLocusNames=At5g41950 {ECO:0000312|Araport:AT5G41950};
GN ORFNames=MJC20.5 {ECO:0000312|EMBL:AED94746.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, DOMAIN, AND
RP INTERACTION WITH BIG5.
RX PubMed=26941089; DOI=10.1105/tpc.15.00794;
RA Sparks J.A., Kwon T., Renna L., Liao F., Brandizzi F., Blancaflor E.B.;
RT "HLB1 is a tetratricopeptide repeat domain-containing protein that operates
RT at the intersection of the exocytic and endocytic pathways at the TGN/EE in
RT Arabidopsis.";
RL Plant Cell 28:746-769(2016).
CC -!- FUNCTION: Component of the endomembrane trafficking machinery that is
CC involved in protein recycling to the plasma membrane. Forms a complex
CC with BIG5/MIN7/BEN1 and actin to modulate the function of the trans-
CC Golgi network /early endosome at the intersection of the exocytic and
CC endocytic pathways. May be linking post-Golgi traffic with the actin
CC cytoskeleton. {ECO:0000269|PubMed:26941089}.
CC -!- SUBUNIT: Interacts with BIG5. {ECO:0000269|PubMed:26941089}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26941089}.
CC Endomembrane system {ECO:0000269|PubMed:26941089}. Golgi apparatus,
CC trans-Golgi network {ECO:0000269|PubMed:26941089}. Early endosome
CC {ECO:0000269|PubMed:26941089}.
CC -!- DOMAIN: The C-terminal domain (496-565) is responsible for the
CC localization to the trans-Golgi network/early endosome.
CC {ECO:0000269|PubMed:26941089}.
CC -!- DISRUPTION PHENOTYPE: Hypersensitivity to latrunculin B1 (LatB) and
CC cytochalasin, reduced primary root length and root hair growth defects.
CC {ECO:0000269|PubMed:26941089}.
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DR EMBL; AB017067; BAB08428.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94746.1; -; Genomic_DNA.
DR EMBL; AY128348; AAM91551.1; -; mRNA.
DR EMBL; BT030345; ABO38758.1; -; mRNA.
DR EMBL; AY085561; AAM62783.1; -; mRNA.
DR RefSeq; NP_199010.1; NM_123560.4.
DR AlphaFoldDB; Q9FHY8; -.
DR SMR; Q9FHY8; -.
DR STRING; 3702.AT5G41950.1; -.
DR iPTMnet; Q9FHY8; -.
DR MetOSite; Q9FHY8; -.
DR PaxDb; Q9FHY8; -.
DR PRIDE; Q9FHY8; -.
DR ProMEX; Q9FHY8; -.
DR ProteomicsDB; 230228; -.
DR EnsemblPlants; AT5G41950.1; AT5G41950.1; AT5G41950.
DR GeneID; 834200; -.
DR Gramene; AT5G41950.1; AT5G41950.1; AT5G41950.
DR KEGG; ath:AT5G41950; -.
DR Araport; AT5G41950; -.
DR TAIR; locus:2165725; AT5G41950.
DR eggNOG; ENOG502QR9B; Eukaryota.
DR HOGENOM; CLU_034799_0_0_1; -.
DR InParanoid; Q9FHY8; -.
DR OMA; TIAPHKH; -.
DR OrthoDB; 671340at2759; -.
DR PhylomeDB; Q9FHY8; -.
DR PRO; PR:Q9FHY8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FHY8; baseline and differential.
DR GO; GO:0005769; C:early endosome; IDA:TAIR.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; IDA:TAIR.
DR GO; GO:0006887; P:exocytosis; IMP:TAIR.
DR GO; GO:0080147; P:root hair cell development; IMP:TAIR.
DR GO; GO:0048768; P:root hair cell tip growth; IMP:TAIR.
DR GO; GO:0048767; P:root hair elongation; IMP:TAIR.
DR GO; GO:0016192; P:vesicle-mediated transport; IDA:TAIR.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Endosome; Golgi apparatus; Membrane; Phosphoprotein;
KW Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..565
FT /note="Protein HLB1"
FT /id="PRO_0000440018"
FT REPEAT 189..222
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 237..270
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 282..315
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT REPEAT 326..359
FT /note="TPR 4"
FT /evidence="ECO:0000255"
FT REPEAT 361..391
FT /note="TPR 5"
FT /evidence="ECO:0000255"
FT REPEAT 392..425
FT /note="TPR 6"
FT /evidence="ECO:0000255"
FT REGION 1..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..91
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..160
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..183
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 479
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862,
FT ECO:0007744|PubMed:19376835"
SQ SEQUENCE 565 AA; 62398 MW; B7A575D4E00BA608 CRC64;
MADTVEEPQL QNGAAPAESE TEQNPIPEPQ LQTEPKLTGE IPEIEADLTP EEVQSEVTDA
KPEEVQSEVK PEEVKTVVTD AKPEEAQSEV KPEEVQSVVT DTKPDLTDVD LSPGGSEEIP
IRSTEVEQES TTSVLKKDDD GNKTFTMREL LSELKSEEGD GTPHSSASPF SRESASQPAE
NNPAMDLINR IQVNDEEGRS RQRVLAFAAR KYASAIERNP DDHDALYNWA LILQESADNV
SPDSVSPSKD DLLEEACKKY DEATRLCPTL YDAYYNWAIA ISDRAKIRGR TKEAEELWEQ
AADNYEKAVQ LNWNSSQALN NWGLVLQELS QIVPAREKEK VVRTAISKFR AAIRLQFDFH
RAIYNLGTVL YGLAEDTLRT GGSGNGKDMP PGELYSQSAI YIAAAHSLKP SYSVYSSALR
LVRSMLPLPH LKVGYLTAPP VGNSLAPHSD WKRTEFELNH ERLLQVLKPE PREMGRNLSG
KAETMSTNVE RKTVKVNITE IVSVTPCADL TLPPGAGLCI DTIHGPVFLV ADSWESLDGW
LDAIRLVYTI YARGKSDVLA GIITG
