ID   3SOFH_NAJAT             Reviewed;          21 AA.
AC   P0C547;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Cardiotoxin-like basic polypeptide ah {ECO:0000303|PubMed:17277458};
DE            Short=CLBPah {ECO:0000303|PubMed:17277458};
DE   Flags: Fragment;
OS   Naja atra (Chinese cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8656;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, TOXIC
RP   DOSE, AND CRYSTALLIZATION.
RC   TISSUE=Venom;
RX   PubMed=17277458; DOI=10.1107/s1744309107002564;
RA   Rong H., Li Y., Lou X.-H., Zhang X., Gao Y.-X., Teng M.-K., Niu L.-W.;
RT   "Purification, partial characterization, crystallization and preliminary X-
RT   ray diffraction of a novel cardiotoxin-like basic protein from Naja naja
RT   atra (South Anhui) venom.";
RL   Acta Crystallogr. F 63:130-134(2007).
CC   -!- FUNCTION: Has hemolytic activity under low-lecithin conditions
CC       (PubMed:17277458). Has low cytotoxic activity (By similarity). Inhibits
CC       the expression of VEGF and bFGF in human non-small-cell lung cancer
CC       cell line NCI-H1299 in a dose-dependent manner (PubMed:17277458).
CC       {ECO:0000250|UniProtKB:P14541, ECO:0000269|PubMed:17277458}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17277458}. Target
CC       cell membrane {ECO:0000250|UniProtKB:P62375}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- PTM: Contains 4 disulfide bonds. {ECO:0000250|UniProtKB:P60301}.
CC   -!- MASS SPECTROMETRY: Mass=7004.877; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:17277458};
CC   -!- TOXIC DOSE: LD(50) is 9.7 mg/kg by intravenous injection into mice.
CC       {ECO:0000269|PubMed:17277458}.
CC   -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since homologs have
CC       a proline residue at position 31. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Orphan group XV sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C547; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Membrane; Secreted;
KW   Target cell membrane; Target membrane; Toxin.
FT   CHAIN           1..>21
FT                   /note="Cardiotoxin-like basic polypeptide ah"
FT                   /evidence="ECO:0000269|PubMed:17277458"
FT                   /id="PRO_0000292041"
FT   NON_TER         21
SQ   SEQUENCE   21 AA;  2400 MW;  F1A9BA86B06896B4 CRC64;
     DCCHNTQLPF IYKTCPEGCN L