ANXA4_PIG
ID ANXA4_PIG Reviewed; 319 AA.
AC P08132; Q29306;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Annexin A4;
DE AltName: Full=35-beta calcimedin;
DE AltName: Full=Annexin IV;
DE AltName: Full=Annexin-4;
DE AltName: Full=Chromobindin-4;
DE AltName: Full=Endonexin I;
DE AltName: Full=Lipocortin IV;
DE AltName: Full=P32.5;
DE AltName: Full=PP4-X;
DE AltName: Full=Placental anticoagulant protein II;
DE Short=PAP-II;
DE AltName: Full=Protein II;
GN Name=ANXA4; Synonyms=ANX4;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP PROTEIN SEQUENCE OF 2-319, ACETYLATION AT ALA-2, AND PHOSPHORYLATION AT
RP THR-7.
RC TISSUE=Intestinal epithelium;
RX PubMed=2956093; DOI=10.1002/j.1460-2075.1987.tb02406.x;
RA Weber K., Johnsson N., Plessmann U., Van P.N., Soling H.-D., Ampe C.,
RA Vandekerckhove J.;
RT "The amino acid sequence of protein II and its phosphorylation site for
RT protein kinase C; the domain structure Ca2+-modulated lipid binding
RT proteins.";
RL EMBO J. 6:1599-1604(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-127.
RC TISSUE=Small intestine;
RX PubMed=8672129; DOI=10.1007/s003359900153;
RA Winteroe A.K., Fredholm M., Davies W.;
RT "Evaluation and characterization of a porcine small intestine cDNA library:
RT analysis of 839 clones.";
RL Mamm. Genome 7:509-517(1996).
CC -!- FUNCTION: Calcium/phospholipid-binding protein which promotes membrane
CC fusion and is involved in exocytosis. {ECO:0000250}.
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Zymogen granule membrane
CC {ECO:0000250|UniProtKB:P50994}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P50994}.
CC -!- DOMAIN: A pair of annexin repeats may form one binding site for calcium
CC and phospholipid.
CC -!- MISCELLANEOUS: Seems to bind one calcium ion with high affinity.
CC -!- SIMILARITY: Belongs to the annexin family. {ECO:0000255|PROSITE-
CC ProRule:PRU01245, ECO:0000305}.
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DR EMBL; F14682; CAA23194.1; -; mRNA.
DR PIR; A27107; LUPG4.
DR RefSeq; NP_001161111.1; NM_001167639.1.
DR AlphaFoldDB; P08132; -.
DR SMR; P08132; -.
DR STRING; 9823.ENSSSCP00000008898; -.
DR iPTMnet; P08132; -.
DR PaxDb; P08132; -.
DR PeptideAtlas; P08132; -.
DR PRIDE; P08132; -.
DR Ensembl; ENSSSCT00015070189; ENSSSCP00015028063; ENSSSCG00015052361.
DR Ensembl; ENSSSCT00025064494; ENSSSCP00025027468; ENSSSCG00025047392.
DR Ensembl; ENSSSCT00040091914; ENSSSCP00040040556; ENSSSCG00040067180.
DR Ensembl; ENSSSCT00050045379; ENSSSCP00050018645; ENSSSCG00050033836.
DR Ensembl; ENSSSCT00055038315; ENSSSCP00055030441; ENSSSCG00055019474.
DR Ensembl; ENSSSCT00055038482; ENSSSCP00055030574; ENSSSCG00055019474.
DR Ensembl; ENSSSCT00065042016; ENSSSCP00065017812; ENSSSCG00065031077.
DR Ensembl; ENSSSCT00065042020; ENSSSCP00065017813; ENSSSCG00065031077.
DR Ensembl; ENSSSCT00065042027; ENSSSCP00065017816; ENSSSCG00065031077.
DR Ensembl; ENSSSCT00065042031; ENSSSCP00065017817; ENSSSCG00065031077.
DR Ensembl; ENSSSCT00065042040; ENSSSCP00065017821; ENSSSCG00065031077.
DR Ensembl; ENSSSCT00065042049; ENSSSCP00065017826; ENSSSCG00065031077.
DR Ensembl; ENSSSCT00070059960; ENSSSCP00070051085; ENSSSCG00070029818.
DR Ensembl; ENSSSCT00070059961; ENSSSCP00070051086; ENSSSCG00070029818.
DR Ensembl; ENSSSCT00070059962; ENSSSCP00070051087; ENSSSCG00070029818.
DR GeneID; 100312960; -.
DR KEGG; ssc:100312960; -.
DR CTD; 307; -.
DR eggNOG; KOG0819; Eukaryota.
DR InParanoid; P08132; -.
DR OrthoDB; 856254at2759; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Chromosome 3.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.220.10; -; 4.
DR InterPro; IPR001464; Annexin.
DR InterPro; IPR018502; Annexin_repeat.
DR InterPro; IPR018252; Annexin_repeat_CS.
DR InterPro; IPR037104; Annexin_sf.
DR InterPro; IPR002391; ANX4.
DR PANTHER; PTHR10502:SF28; PTHR10502:SF28; 1.
DR Pfam; PF00191; Annexin; 4.
DR PRINTS; PR00196; ANNEXIN.
DR SMART; SM00335; ANX; 4.
DR SUPFAM; SSF47874; SSF47874; 1.
DR PROSITE; PS00223; ANNEXIN_1; 4.
DR PROSITE; PS51897; ANNEXIN_2; 4.
PE 1: Evidence at protein level;
KW Acetylation; Annexin; Calcium; Calcium/phospholipid-binding;
KW Cytoplasmic vesicle; Direct protein sequencing; Membrane; Phosphoprotein;
KW Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2956093"
FT CHAIN 2..319
FT /note="Annexin A4"
FT /id="PRO_0000067484"
FT REPEAT 14..85
FT /note="Annexin 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01245"
FT REPEAT 86..157
FT /note="Annexin 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01245"
FT REPEAT 169..241
FT /note="Annexin 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01245"
FT REPEAT 245..316
FT /note="Annexin 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01245"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:2956093"
FT MOD_RES 7
FT /note="Phosphothreonine; by PKC"
FT /evidence="ECO:0000269|PubMed:2956093"
FT MOD_RES 12
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09525"
FT MOD_RES 213
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P09525"
FT MOD_RES 293
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P09525"
FT MOD_RES 300
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P09525"
SQ SEQUENCE 319 AA; 35829 MW; DB55FC634EFFD30A CRC64;
MAAKGGTVKA ASGFNAAEDA QTLRKAMKGL GTDEDAIISV LAYRSTAQRQ EIRTAYKSTI
GRDLLDDLKS ELSGNFEQVI LGMMTPTVLY DVQELRRAMK GAGTDEGCLI EILASRTPEE
IRRINQTYQL QYGRSLEDDI RSDTSFMFQR VLVSLSAGGR DEGNYLDDAL VRQDAQDLYE
AGEKKWGTDE VKFLTVLCSR NRNHLLHVFD EYKRISQKDI EQSIKSETSG SFEDALLAIV
KCMRNKSAYF AERLYKSMKG LGTDDNTLIR VMVSRAEIDM MDIRANFKRL YGKSLYSFIK
GDTSGDYRKV LLILCGGDD
