HLES_DROME
ID HLES_DROME Reviewed; 1077 AA.
AC Q02308; A4V358; A4V359; Q6AWM8; Q960W5; Q9VDK0; Q9VDK1;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Protein hairless;
GN Name=H; ORFNames=CG5460;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Imaginal disk;
RX PubMed=1516831; DOI=10.1101/gad.6.9.1752;
RA Bang A.G., Posakony J.W.;
RT "The Drosophila gene Hairless encodes a novel basic protein that controls
RT alternative cell fates in adult sensory organ development.";
RL Genes Dev. 6:1752-1769(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=1419850; DOI=10.1016/0925-4773(92)90006-6;
RA Maier D., Stumm G., Kuhn K., Preiss A.;
RT "Hairless, a Drosophila gene involved in neural development, encodes a
RT novel, serine rich protein.";
RL Mech. Dev. 38:143-156(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 265-1077.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-720; SER-723; SER-746 AND
RP SER-753, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Is a potent antagonist of neurogenic gene activity during
CC sensory organ development. The expression of distinct cell fates by the
CC trichogen (shaft) / tormogen (socket) sister cell pair depends on the
CC level of H activity. A certain threshold level of H activity is
CC required, below which both sister cells adopt the tormogen fate.
CC {ECO:0000269|PubMed:1419850, ECO:0000269|PubMed:1516831}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A; Synonyms=D;
CC IsoId=Q02308-1; Sequence=Displayed;
CC Name=B; Synonyms=C;
CC IsoId=Q02308-2; Sequence=VSP_006952;
CC -!- TISSUE SPECIFICITY: During embryogenesis expression is primarily in
CC endo- and mesodermal cell layers. Ovary, embryos, larval and pupal
CC imaginal disks. {ECO:0000269|PubMed:1419850,
CC ECO:0000269|PubMed:1516831}.
CC -!- DEVELOPMENTAL STAGE: Expression peaks during embryogenesis and lowest
CC during larval stages. {ECO:0000269|PubMed:1419850}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA28607.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAK93230.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M95192; AAA28607.1; ALT_INIT; mRNA.
DR EMBL; X67239; CAA47664.1; -; mRNA.
DR EMBL; AE014297; AAF55790.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF55791.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN14357.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN14358.1; -; Genomic_DNA.
DR EMBL; BT015220; AAT94449.1; -; mRNA.
DR EMBL; AY051806; AAK93230.1; ALT_INIT; mRNA.
DR PIR; A44067; A44067.
DR RefSeq; NP_524418.2; NM_079694.3. [Q02308-2]
DR RefSeq; NP_732533.1; NM_169907.2. [Q02308-1]
DR RefSeq; NP_732534.1; NM_169908.2. [Q02308-1]
DR RefSeq; NP_732535.1; NM_169909.2. [Q02308-2]
DR PDB; 5E24; X-ray; 2.14 A; B/D=232-269.
DR PDBsum; 5E24; -.
DR AlphaFoldDB; Q02308; -.
DR SMR; Q02308; -.
DR BioGRID; 67421; 145.
DR DIP; DIP-39152N; -.
DR ELM; Q02308; -.
DR IntAct; Q02308; 4.
DR STRING; 7227.FBpp0083322; -.
DR iPTMnet; Q02308; -.
DR PaxDb; Q02308; -.
DR EnsemblMetazoa; FBtr0083914; FBpp0083322; FBgn0001169. [Q02308-1]
DR EnsemblMetazoa; FBtr0083915; FBpp0083323; FBgn0001169. [Q02308-2]
DR EnsemblMetazoa; FBtr0083916; FBpp0083324; FBgn0001169. [Q02308-1]
DR EnsemblMetazoa; FBtr0083917; FBpp0083325; FBgn0001169. [Q02308-2]
DR GeneID; 42445; -.
DR KEGG; dme:Dmel_CG5460; -.
DR UCSC; CG5460-RA; d. melanogaster.
DR FlyBase; FBgn0001169; H.
DR VEuPathDB; VectorBase:FBgn0001169; -.
DR eggNOG; ENOG502S24X; Eukaryota.
DR HOGENOM; CLU_282042_0_0_1; -.
DR InParanoid; Q02308; -.
DR OMA; YHRPRNS; -.
DR PhylomeDB; Q02308; -.
DR SignaLink; Q02308; -.
DR BioGRID-ORCS; 42445; 0 hits in 3 CRISPR screens.
DR ChiTaRS; h; fly.
DR GenomeRNAi; 42445; -.
DR PRO; PR:Q02308; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0001169; Expressed in cleaving embryo and 41 other tissues.
DR ExpressionAtlas; Q02308; baseline and differential.
DR Genevisible; Q02308; DM.
DR GO; GO:0090571; C:RNA polymerase II transcription repressor complex; IPI:FlyBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:FlyBase.
DR GO; GO:0008587; P:imaginal disc-derived wing margin morphogenesis; IMP:FlyBase.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IMP:FlyBase.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IGI:FlyBase.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0008052; P:sensory organ boundary specification; IMP:FlyBase.
DR GO; GO:0016360; P:sensory organ precursor cell fate determination; IMP:FlyBase.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IMP:FlyBase.
DR GO; GO:0048190; P:wing disc dorsal/ventral pattern formation; IMP:FlyBase.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; DNA-binding;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..1077
FT /note="Protein hairless"
FT /id="PRO_0000083990"
FT REGION 14..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 97..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 269..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 340..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 408..504
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 533..876
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 976..1077
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..141
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..315
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..436
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..472
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..587
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 639..685
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 700..747
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 772..801
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 809..825
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 860..876
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1011..1077
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 720
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 723
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 746
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 753
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..18
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_006952"
FT CONFLICT 8
FT /note="T -> S (in Ref. 5; AAT94449)"
FT /evidence="ECO:0000305"
FT CONFLICT 680
FT /note="F -> S (in Ref. 2; CAA47664)"
FT /evidence="ECO:0000305"
FT CONFLICT 914
FT /note="L -> P (in Ref. 5; AAT94449)"
FT /evidence="ECO:0000305"
FT STRAND 233..239
FT /evidence="ECO:0007829|PDB:5E24"
FT STRAND 242..249
FT /evidence="ECO:0007829|PDB:5E24"
FT STRAND 252..254
FT /evidence="ECO:0007829|PDB:5E24"
FT STRAND 258..260
FT /evidence="ECO:0007829|PDB:5E24"
SQ SEQUENCE 1077 AA; 111100 MW; E8B846C0E2E575D4 CRC64;
MALLNDVTSV AECNRQTTMT DEHKSNINSN SSHSSNNNNN GSSSNNDNNS NDDAASSSNS
KNNNTSNESS HSNNNTSSII AEAAAKFLLK NGLNGSSSTS YPPLPPPLPA NLSRTTTPTT
TTTPSSSSST ASNGFLPHAK TPKSSSIMAA SAAVAASVVG ATASKPTIDV LGGVLDYSSL
GGAATGSLPT TAVVAAAAGT AKIGKGSNSG GSFDMGRTPI STHGNNSWGG YGGRLQFFKD
GKFILELARS KDGDKSGWVS VTRKTFRPPS AATSATVTPT SAVTTAYPKN ENSTSLSFSD
DNSSIQSSPW QRDQPWKQSR PRRGISKELS LFFHRPRNST LGRAALRTAA RKRRRPHEPL
TTSEDQQPIF ATAIKAENGD DTLKAEAAEA VEIENVAVAD TTTNEIKIEK PDTIKGEDDA
ERLEKEPKKA VSDDSESKEA SPGQQVEPQP KDETVDVEMK MNTSEDEEPM TELPRITNAV
NGDLNGDLKA SIGKPKSKPK PKAKLSSIIQ KLIDSVPARL EQMSKTSAVI ASTTTSSDRI
GGGLSHALTH KVSPPSSATA AGRLVEYHTQ HVSPRKRILR EFEKVSLEDN GCVNNGSGGA
SSGGAGGKRS RAKGTSTSSP AGKASPMNLA PPQGKPSPSP GSSSSSTSPA TLSTQPTRLN
SSYSIHSLLG GSSGSGSSSF SSSGKKCGDH PAAIISNVHH PQHSMYQPSS SSYPRALLTS
PKSPDVSGSN GGGGKSPSHT GTKKRSPPYS AGSPVDYGHS FYRDPYAGAG RPSTSGSASQ
DLSPPRSSPA SPATTPRTVP KKTASIRREF ASPSASSSSC PSPGDRSASP PERRHMQQQP
HLQRSSPLHY YMYPPPPQVN GNGSAGSPTS APPTSNSSAA AVAAAAAAAA AYIPSPSIYN
PYISTLAALR HNPLWMHHYQ TGASPLLSPH PQPGGSAAAA AAAAAARLSP QSAYHAFAYN
GVGAAVAAAA AAAAFGQPAP SPHTHPHLAH PHQHPHPAAL TTHHSPAHLA TPKLTDSSTD
QMSATSSHRT ASTSPSSSSA SASSSAATSG ASSSAMFHTS SLRNEQSSDL PLNLSKH
