HLF_MOUSE
ID HLF_MOUSE Reviewed; 295 AA.
AC Q8BW74; Q6PF83;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Hepatic leukemia factor;
GN Name=Hlf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Diencephalon, and Oviduct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INVOLVEMENT IN EPILEPSY.
RX PubMed=15175240; DOI=10.1101/gad.301404;
RA Gachon F., Fonjallaz P., Damiola F., Gos P., Kodama T., Zakany J.,
RA Duboule D., Petit B., Tafti M., Schibler U.;
RT "The loss of circadian PAR bZip transcription factors results in
RT epilepsy.";
RL Genes Dev. 18:1397-1412(2004).
CC -!- SUBUNIT: Binds DNA specifically as homodimer or heterodimer with other
CC PAR factors. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Accumulates according to a robust circadian rhythm. Displays
CC only a moderate amplitude in the liver (1.6-fold) and none in the
CC brain.
CC -!- MISCELLANEOUS: Mice deficient for all three PAR bZIP proteins (DBP, HLF
CC and TEF) display a dramatically shortened life span and are highly
CC susceptible to generalized spontaneous and audiogenic epilepsies (due
CC for example to the noise of a vacuum cleaner) that are frequently
CC lethal. The down-regulation of pyridoxal kinase (Pdxk) expression in
CC these mice may participate in this seizure phenotype.
CC -!- SIMILARITY: Belongs to the bZIP family. PAR subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH57693.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK054062; BAC35642.1; -; mRNA.
DR EMBL; BC057693; AAH57693.1; ALT_INIT; mRNA.
DR EMBL; BC058705; AAH58705.1; -; mRNA.
DR CCDS; CCDS25241.1; -.
DR RefSeq; NP_766151.1; NM_172563.3.
DR RefSeq; XP_006533068.1; XM_006533005.3.
DR RefSeq; XP_006533069.1; XM_006533006.1.
DR AlphaFoldDB; Q8BW74; -.
DR SMR; Q8BW74; -.
DR BioGRID; 229842; 8.
DR IntAct; Q8BW74; 5.
DR STRING; 10090.ENSMUSP00000004051; -.
DR CarbonylDB; Q8BW74; -.
DR iPTMnet; Q8BW74; -.
DR PhosphoSitePlus; Q8BW74; -.
DR PaxDb; Q8BW74; -.
DR PRIDE; Q8BW74; -.
DR ProteomicsDB; 273147; -.
DR Antibodypedia; 30848; 189 antibodies from 27 providers.
DR DNASU; 217082; -.
DR Ensembl; ENSMUST00000004051; ENSMUSP00000004051; ENSMUSG00000003949.
DR GeneID; 217082; -.
DR KEGG; mmu:217082; -.
DR UCSC; uc007kwr.1; mouse.
DR CTD; 3131; -.
DR MGI; MGI:96108; Hlf.
DR VEuPathDB; HostDB:ENSMUSG00000003949; -.
DR eggNOG; KOG3119; Eukaryota.
DR GeneTree; ENSGT00940000156555; -.
DR HOGENOM; CLU_051922_2_0_1; -.
DR InParanoid; Q8BW74; -.
DR OMA; HASTSVH; -.
DR OrthoDB; 1023460at2759; -.
DR PhylomeDB; Q8BW74; -.
DR TreeFam; TF315869; -.
DR BioGRID-ORCS; 217082; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Hlf; mouse.
DR PRO; PR:Q8BW74; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8BW74; protein.
DR Bgee; ENSMUSG00000003949; Expressed in lateral geniculate body and 195 other tissues.
DR ExpressionAtlas; Q8BW74; baseline and differential.
DR Genevisible; Q8BW74; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; ISO:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; IMP:MGI.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR029829; HLF.
DR InterPro; IPR040223; PAR_bZIP.
DR PANTHER; PTHR11988; PTHR11988; 1.
DR PANTHER; PTHR11988:SF28; PTHR11988:SF28; 1.
DR Pfam; PF07716; bZIP_2; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 2: Evidence at transcript level;
KW Biological rhythms; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..295
FT /note="Hepatic leukemia factor"
FT /id="PRO_0000076511"
FT DOMAIN 225..288
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 36..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 227..247
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 248..255
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 36..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 27..38
FT /note="ENPLKLPLHPED -> KKQSSPFPFSSS (in Ref. 2; AAH57693)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="K -> KQ (in Ref. 2; AAH57693)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 295 AA; 33149 MW; D57CAB8601A954B4 CRC64;
MEKMSRQLPL NPTFIPPPYG VLRSLLENPL KLPLHPEDAF SKEKDKGKKL DDESSSPTVP
QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSTA
PSVMDLSSRA TAPLHPGIPS PNCMQSPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA
DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK
RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPL
