HLF_RAT
ID HLF_RAT Reviewed; 295 AA.
AC Q64709; Q64708;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 138.
DE RecName: Full=Hepatic leukemia factor;
GN Name=Hlf;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS HLF43; HLF17 AND HLF24), AND
RP ALTERNATIVE INITIATION.
RC TISSUE=Liver;
RX PubMed=7556072; DOI=10.1002/j.1460-2075.1995.tb00105.x;
RA Falvey E., Fleury-Olela F., Schibler U.;
RT "The rat hepatic leukemia factor (HLF) gene encodes two transcriptional
RT activators with distinct circadian rhythms, tissue distributions and target
RT preferences.";
RL EMBO J. 14:4307-4317(1995).
CC -!- SUBUNIT: Binds DNA specifically as homodimer or heterodimer with other
CC PAR factors. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing, Alternative initiation; Named isoforms=4;
CC Name=HLF43;
CC IsoId=Q64709-1; Sequence=Displayed;
CC Name=HLF17;
CC IsoId=Q64709-2; Sequence=VSP_018687, VSP_018688, VSP_000585;
CC Name=HLF24;
CC IsoId=Q64709-3; Sequence=VSP_000584;
CC Name=HLF36;
CC IsoId=Q64709-4; Sequence=VSP_018687, VSP_018688;
CC -!- TISSUE SPECIFICITY: Isoform HLF43 is abundant in brain, liver and
CC kidney. Isoform HLF36 is expressed only in the liver. Both isoforms
CC accumulate in the liver with different circadian amplitudes. Isoform
CC HLF36 reaches peak expression levels between 8 and 12 p.m. Isoform
CC HLF43 displays a more pronounced fluctuation through the day.
CC -!- INDUCTION: Accumulates according to a robust circadian rhythm.
CC -!- MISCELLANEOUS: [Isoform HLF17]: Produced by alternative initiation at
CC an unusual initiation codon at position 50. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform HLF24]: Produced by alternative splicing.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform HLF36]: Produced by alternative initiation at
CC an unusual initiation codon at position 50. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the bZIP family. PAR subfamily. {ECO:0000305}.
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DR EMBL; S79820; AAB35322.1; -; mRNA.
DR PIR; S58525; S58525.
DR AlphaFoldDB; Q64709; -.
DR SMR; Q64709; -.
DR STRING; 10116.ENSRNOP00000052527; -.
DR iPTMnet; Q64709; -.
DR PhosphoSitePlus; Q64709; -.
DR PaxDb; Q64709; -.
DR RGD; 1582828; Hlf.
DR eggNOG; KOG3119; Eukaryota.
DR InParanoid; Q64709; -.
DR PhylomeDB; Q64709; -.
DR PRO; PR:Q64709; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; ISO:RGD.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR029829; HLF.
DR InterPro; IPR040223; PAR_bZIP.
DR PANTHER; PTHR11988; PTHR11988; 1.
DR PANTHER; PTHR11988:SF28; PTHR11988:SF28; 1.
DR Pfam; PF07716; bZIP_2; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 2: Evidence at transcript level;
KW Alternative initiation; Alternative splicing; Biological rhythms;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..295
FT /note="Hepatic leukemia factor"
FT /id="PRO_0000003514"
FT DOMAIN 225..288
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 34..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 93..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 227..247
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 248..255
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 36..52
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..49
FT /note="Missing (in isoform HLF17 and isoform HLF36)"
FT /evidence="ECO:0000303|PubMed:7556072"
FT /id="VSP_018687"
FT VAR_SEQ 50
FT /note="L -> M (in isoform HLF17 and isoform HLF36)"
FT /evidence="ECO:0000303|PubMed:7556072"
FT /id="VSP_018688"
FT VAR_SEQ 170..295
FT /note="Missing (in isoform HLF24)"
FT /evidence="ECO:0000303|PubMed:7556072"
FT /id="VSP_000584"
FT VAR_SEQ 171..295
FT /note="Missing (in isoform HLF17)"
FT /evidence="ECO:0000303|PubMed:7556072"
FT /id="VSP_000585"
SQ SEQUENCE 295 AA; 33114 MW; 4057B2421F55A902 CRC64;
MEKMSRQLPL NPTFIPPPYG VLRSLLENPL KLPLHPEDAF SKDRDKGKKL DDGSNSPTVP
QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSTA
PSVMDLSSRA TAPLHPGIPS PNCMQNPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA
DLALSSIPGP EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK
RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPL
