HLGA_STAAM
ID HLGA_STAAM Reviewed; 309 AA.
AC P0A071; P31714; Q07225; Q53689; Q53690;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Gamma-hemolysin component A;
DE AltName: Full=H-gamma-2;
DE AltName: Full=H-gamma-II;
DE Flags: Precursor;
GN Name=hlgA; Synonyms=hlg2; OrderedLocusNames=SAV2419;
OS Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158878;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu50 / ATCC 700699;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
CC -!- FUNCTION: Toxin that seems to act by forming pores in the membrane of
CC the cell. Has a hemolytic and a leucotoxic activity (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Toxicity requires sequential binding and synergistic
CC association of a class S and a class F component which form
CC heterooligomeric complexes. HlgA (class S) associates with HlgB (class
CC F) thus forming an AB toxin in strains producing both gamma-hemolysins
CC and leukocidins. HlgA and LukF-PV can also form a complex (By
CC similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC P0A071; A0A0H3JX61: hlgB; NbExp=2; IntAct=EBI-16223478, EBI-16223472;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the aerolysin family. {ECO:0000305}.
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DR EMBL; BA000017; BAB58581.1; -; Genomic_DNA.
DR RefSeq; WP_000594519.1; NC_002758.2.
DR PDB; 3B07; X-ray; 2.50 A; B/D/F/H=30-309.
DR PDB; 4P1Y; X-ray; 2.99 A; B/D/F/H=42-309.
DR PDBsum; 3B07; -.
DR PDBsum; 4P1Y; -.
DR AlphaFoldDB; P0A071; -.
DR SMR; P0A071; -.
DR DIP; DIP-59713N; -.
DR IntAct; P0A071; 1.
DR PaxDb; P0A071; -.
DR EnsemblBacteria; BAB58581; BAB58581; SAV2419.
DR KEGG; sav:SAV2419; -.
DR HOGENOM; CLU_075311_0_0_9; -.
DR OMA; ANPFTEI; -.
DR PhylomeDB; P0A071; -.
DR BioCyc; SAUR158878:SAV_RS13185-MON; -.
DR Proteomes; UP000002481; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR InterPro; IPR003963; Bi-component_toxin_staph.
DR InterPro; IPR016183; Leukocidin/Hemolysin_toxin.
DR InterPro; IPR036435; Leukocidin/porin_MspA_sf.
DR Pfam; PF07968; Leukocidin; 1.
DR PRINTS; PR01468; BICOMPNTOXIN.
DR SUPFAM; SSF56959; SSF56959; 1.
DR TIGRFAMs; TIGR01002; hlyII; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytolysis; Hemolysis; Secreted; Signal; Toxin; Virulence.
FT SIGNAL 1..29
FT /evidence="ECO:0000250"
FT CHAIN 30..309
FT /note="Gamma-hemolysin component A"
FT /id="PRO_0000018419"
FT STRAND 42..51
FT /evidence="ECO:0007829|PDB:3B07"
FT TURN 52..55
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 56..65
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 70..84
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 88..91
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 98..113
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 118..125
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 131..147
FT /evidence="ECO:0007829|PDB:3B07"
FT TURN 148..150
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 151..179
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 182..189
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 191..194
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 197..200
FT /evidence="ECO:0007829|PDB:3B07"
FT TURN 204..207
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 212..215
FT /evidence="ECO:0007829|PDB:3B07"
FT HELIX 216..219
FT /evidence="ECO:0007829|PDB:3B07"
FT HELIX 223..225
FT /evidence="ECO:0007829|PDB:3B07"
FT HELIX 228..231
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 237..244
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 246..248
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 250..270
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 273..295
FT /evidence="ECO:0007829|PDB:3B07"
FT TURN 296..299
FT /evidence="ECO:0007829|PDB:3B07"
FT STRAND 300..307
FT /evidence="ECO:0007829|PDB:3B07"
SQ SEQUENCE 309 AA; 34956 MW; 15CAE12E1025B616 CRC64;
MIKNKILTAT LAVGLIAPLA NPFIEISKAE NKIEDIGQGA EIIKRTQDIT SKRLAITQNI
QFDFVKDKKY NKDALVVKMQ GFISSRTTYS DLKKYPYIKR MIWPFQYNIS LKTKDSNVDL
INYLPKNKID SADVSQKLGY NIGGNFQSAP SIGGSGSFNY SKTISYNQKN YVTEVESQNS
KGVKWGVKAN SFVTPNGQVS AYDQYLFAQD PTGPAARDYF VPDNQLPPLI QSGFNPSFIT
TLSHERGKGD KSEFEITYGR NMDATYAYVT RHRLAVDRKH DAFKNRNVTV KYEVNWKTHE
VKIKSITPK
