HLH12_CAEEL
ID HLH12_CAEEL Reviewed; 150 AA.
AC Q18277;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Helix-loop-helix protein hlh-12 {ECO:0000305};
GN Name=hlh-12 {ECO:0000312|WormBase:C28C12.8};
GN Synonyms=mig-24 {ECO:0000312|WormBase:C28C12.8};
GN ORFNames=C28C12.8 {ECO:0000312|WormBase:C28C12.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH HLH-2, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17588558; DOI=10.1016/j.ydbio.2007.05.024;
RA Tamai K.K., Nishiwaki K.;
RT "bHLH transcription factors regulate organ morphogenesis via activation of
RT an ADAMTS protease in C. elegans.";
RL Dev. Biol. 308:562-571(2007).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25982859; DOI=10.1016/j.gene.2015.05.030;
RA Meighan C.M., Kann A.P., Egress E.R.;
RT "Transcription factor hlh-2/E/Daughterless drives expression of alpha
RT integrin ina-1 during DTC migration in C. elegans.";
RL Gene 568:220-226(2015).
CC -!- FUNCTION: Transcription factor which binds the E box motif 5'-GCAGGTG-
CC 3' (PubMed:17588558). Involved in migration of the gonadal leader
CC cells; distal tip cells (DTCs) in hermaphrodites, and linker cells in
CC males (PubMed:25982859, PubMed:17588558). Positively regulates
CC expression of alpha integrin ina-1 and ADAMTS protease gon-1
CC (PubMed:25982859, PubMed:17588558). {ECO:0000269|PubMed:17588558,
CC ECO:0000269|PubMed:25982859}.
CC -!- SUBUNIT: Forms a heterodimer with helix-loop-helix protein hlh-2.
CC {ECO:0000269|PubMed:17588558}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DEVELOPMENTAL STAGE: First expressed in the gonadal leader cells, the
CC distal tip cells (DTCs) in hermaphrodites and linker cells in males,
CC during the early larval L2 stage (PubMed:17588558). Expression in the
CC linker cells in males continues until larval stage L4
CC (PubMed:17588558). Expression in DTCs in hermaphrodites continues until
CC adulthood (PubMed:17588558). {ECO:0000269|PubMed:17588558}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown reduces expression of
CC alpha integrin ina-1 and ADAMTS protease gon-1, and causes defects in
CC migration of the gonadal distal tip cells (DTCs).
CC {ECO:0000269|PubMed:17588558, ECO:0000269|PubMed:25982859}.
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DR EMBL; BX284604; CCD64010.1; -; Genomic_DNA.
DR PIR; T15675; T15675.
DR RefSeq; NP_501445.1; NM_069044.3.
DR AlphaFoldDB; Q18277; -.
DR SMR; Q18277; -.
DR IntAct; Q18277; 1.
DR STRING; 6239.C28C12.8; -.
DR PaxDb; Q18277; -.
DR EnsemblMetazoa; C28C12.8.1; C28C12.8.1; WBGene00001956.
DR GeneID; 182980; -.
DR KEGG; cel:CELE_C28C12.8; -.
DR UCSC; C28C12.8; c. elegans.
DR CTD; 182980; -.
DR WormBase; C28C12.8; CE04115; WBGene00001956; hlh-12.
DR eggNOG; ENOG502TIDB; Eukaryota.
DR GeneTree; ENSGT00990000206751; -.
DR HOGENOM; CLU_1827095_0_0_1; -.
DR InParanoid; Q18277; -.
DR OMA; AFISRHE; -.
DR OrthoDB; 1847271at2759; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00001956; Expressed in larva and 1 other tissue.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0035262; P:gonad morphogenesis; IMP:UniProtKB.
DR GO; GO:1903356; P:positive regulation of distal tip cell migration; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..150
FT /note="Helix-loop-helix protein hlh-12"
FT /id="PRO_0000453282"
FT DOMAIN 13..65
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 13..26
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 27..65
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 150 AA; 17270 MW; 4618862913BA1A41 CRC64;
MAKKPRVTKL NTDRRSRANE RERQRVSEMN GMFDVLLNLL PPSHFKTRLS RVQILREATS
YIIRLHNFLI ESSNSDIDAI TVFPHIFNGE RKSNKDAIRR PMKLKQGGGV AAFISRHELP
PLQLPNPVIP ILKPTSVPVW PQTNVYIAYF
