HLH14_CAEEL
ID HLH14_CAEEL Reviewed; 148 AA.
AC Q09961;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 11-NOV-2015, sequence version 3.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Helix-loop-helix protein 14 {ECO:0000303|PubMed:14627726};
GN Name=hlh-14 {ECO:0000312|WormBase:C18A3.8};
GN ORFNames=C18A3.8 {ECO:0000312|WormBase:C18A3.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP LEU-27 AND 54-GLN--HIS-148.
RX PubMed=14627726; DOI=10.1242/dev.00894;
RA Frank C.A., Baum P.D., Garriga G.;
RT "HLH-14 is a C. elegans achaete-scute protein that promotes neurogenesis
RT through asymmetric cell division.";
RL Development 130:6507-6518(2003).
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21698137; DOI=10.1371/journal.pgen.1002109;
RA Poole R.J., Bashllari E., Cochella L., Flowers E.B., Hobert O.;
RT "A Genome-Wide RNAi Screen for Factors Involved in Neuronal Specification
RT in Caenorhabditis elegans.";
RL PLoS Genet. 7:e1002109-e1002109(2011).
CC -!- FUNCTION: Probable transcription factor, involved in determining
CC neuroblast cell fate, morphogenesis and aspects of terminal
CC differentiation in both left/right symmetric and asymmetric neuronal
CC lineages. {ECO:0000269|PubMed:14627726, ECO:0000269|PubMed:21698137}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:14627726}.
CC -!- DEVELOPMENTAL STAGE: Expressed in both symmetric and asymmetric
CC neuronal lineages (PubMed:21698137). In the posterior embryo, expressed
CC in PVQ/HSN/PHB neuroblasts and their descendants (PubMed:14627726,
CC PubMed:21698137). Expressed in the sensory ASE neuron lineage
CC (PubMed:21698137). {ECO:0000269|PubMed:14627726,
CC ECO:0000269|PubMed:21698137}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown disrupts differentiation
CC of ASE L/R sensory neurons. {ECO:0000269|PubMed:21698137}.
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DR EMBL; BX284602; CCD65023.2; -; Genomic_DNA.
DR PIR; T15548; T15548.
DR RefSeq; NP_495131.3; NM_062730.5.
DR AlphaFoldDB; Q09961; -.
DR SMR; Q09961; -.
DR IntAct; Q09961; 1.
DR STRING; 6239.C18A3.8; -.
DR PaxDb; Q09961; -.
DR EnsemblMetazoa; C18A3.8.1; C18A3.8.1; WBGene00001958.
DR GeneID; 182758; -.
DR KEGG; cel:CELE_C18A3.8; -.
DR UCSC; C18A3.8; c. elegans.
DR CTD; 182758; -.
DR WormBase; C18A3.8; CE50415; WBGene00001958; hlh-14.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000170891; -.
DR HOGENOM; CLU_1129946_0_0_1; -.
DR InParanoid; Q09961; -.
DR OMA; YSPTQMC; -.
DR OrthoDB; 1592204at2759; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001958; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:WormBase.
DR GO; GO:0010171; P:body morphogenesis; IMP:WormBase.
DR GO; GO:0040011; P:locomotion; IMP:WormBase.
DR GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR GO; GO:0014018; P:neuroblast fate specification; IMP:WormBase.
DR GO; GO:0001764; P:neuron migration; IMP:WormBase.
DR GO; GO:0018991; P:oviposition; IMP:WormBase.
DR GO; GO:0040010; P:positive regulation of growth rate; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009786; P:regulation of asymmetric cell division; IMP:WormBase.
DR GO; GO:0045664; P:regulation of neuron differentiation; IGI:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:WormBase.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..148
FT /note="Helix-loop-helix protein 14"
FT /evidence="ECO:0000305"
FT /id="PRO_0000452179"
FT DOMAIN 4..56
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4..17
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 18..56
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 63..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 112..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 27
FT /note="L->F: In gm34; absent HSN motoneurons, PHB sensory
FT neurons and PVQ neurons."
FT /evidence="ECO:0000269|PubMed:14627726"
FT MUTAGEN 54..148
FT /note="Missing: In ju243; absent PHB sensory neurons."
FT /evidence="ECO:0000269|PubMed:14627726"
SQ SEQUENCE 148 AA; 17148 MW; EEC47265E9177EE7 CRC64;
MAKKNQVARN ERERKRVHQV NHGFDVLRNR LQPKNHTKKW SKADTLREAV KYIQQLQVLL
NQDPQQPSVS SSTPDYTMNN SNNFNNYAVK EEFSMYLPQN YCPQNQMSVP HGDVSHNFNS
PTSSVSSSSY SPTQMCYPPV SYSNYPHH
