HLH15_CAEEL
ID HLH15_CAEEL Reviewed; 89 AA.
AC Q18590;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Helix-loop-helix protein 15 {ECO:0000305};
GN Name=hlh-15 {ECO:0000312|WormBase:C43H6.8};
GN ORFNames=C43H6.8 {ECO:0000312|WormBase:C43H6.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=19632181; DOI=10.1016/j.cell.2009.04.058;
RA Grove C.A., De Masi F., Barrasa M.I., Newburger D.E., Alkema M.J.,
RA Bulyk M.L., Walhout A.J.M.;
RT "A multiparameter network reveals extensive divergence between C. elegans
RT bHLH transcription factors.";
RL Cell 138:314-327(2009).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=26620638; DOI=10.1038/ncomms10043;
RA Mansfeld J., Urban N., Priebe S., Groth M., Frahm C., Hartmann N.,
RA Gebauer J., Ravichandran M., Dommaschk A., Schmeisser S., Kuhlow D.,
RA Monajembashi S., Bremer-Streck S., Hemmerich P., Kiehntopf M., Zamboni N.,
RA Englert C., Guthke R., Kaleta C., Platzer M., Suehnel J., Witte O.W.,
RA Zarse K., Ristow M.;
RT "Branched-chain amino acid catabolism is a conserved regulator of
RT physiological ageing.";
RL Nat. Commun. 6:10043-10043(2015).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=32203922; DOI=10.1016/j.redox.2020.101448;
RA Rozanov L., Ravichandran M., Grigolon G., Zanellati M.C., Mansfeld J.,
RA Zarse K., Barzilai N., Atzmon G., Fischer F., Ristow M.;
RT "Redox-mediated regulation of aging and healthspan by an evolutionarily
RT conserved transcription factor HLH-2/Tcf3/E2A.";
RL Redox Biol. 32:101448-101448(2020).
CC -!- FUNCTION: Transcription factor which binds the E box motif 5'-
CC CA[TC][AG]TG-3' (By similarity). Involved in modulating physiological
CC aging, probably by regulating expression of branched-chain amino acid
CC transferase-1, bcat-1 (PubMed:26620638, PubMed:32203922).
CC {ECO:0000250|UniProtKB:Q64221, ECO:0000269|PubMed:26620638,
CC ECO:0000269|PubMed:32203922}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in sensory head neurons of the lateral
CC ganglion. {ECO:0000269|PubMed:19632181}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown extends mean lifespan and
CC reduces expression of bcat-1 by >50%. {ECO:0000269|PubMed:26620638,
CC ECO:0000269|PubMed:32203922}.
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DR EMBL; BX284606; CCD67185.1; -; Genomic_DNA.
DR PIR; T29995; T29995.
DR RefSeq; NP_508440.1; NM_076039.1.
DR AlphaFoldDB; Q18590; -.
DR SMR; Q18590; -.
DR IntAct; Q18590; 1.
DR STRING; 6239.C43H6.8; -.
DR PaxDb; Q18590; -.
DR EnsemblMetazoa; C43H6.8.1; C43H6.8.1; WBGene00001959.
DR GeneID; 183427; -.
DR KEGG; cel:CELE_C43H6.8; -.
DR UCSC; C43H6.8; c. elegans.
DR CTD; 183427; -.
DR WormBase; C43H6.8; CE06959; WBGene00001959; hlh-15.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000164480; -.
DR HOGENOM; CLU_171328_1_1_1; -.
DR InParanoid; Q18590; -.
DR OMA; RVESFNM; -.
DR OrthoDB; 1185741at2759; -.
DR PhylomeDB; Q18590; -.
DR PRO; PR:Q18590; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001959; Expressed in pharyngeal muscle cell (C elegans) and 2 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:WormBase.
DR GO; GO:0007568; P:aging; IMP:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:WormBase.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR040238; TAL-like.
DR PANTHER; PTHR13864; PTHR13864; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..89
FT /note="Helix-loop-helix protein 15"
FT /id="PRO_0000451921"
FT DOMAIN 32..84
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 32..45
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 46..84
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 89 AA; 10557 MW; 61EEB717ED64F20E CRC64;
MLMEDGGLDT TSEEYRKLSK AERRKRRRAT PKYRNLHATR ERIRVESFNM AFSQLRALLP
TLPVEKKLSK IEILRFSIAY ISFLDNLLQ
