HLH25_CAEEL
ID HLH25_CAEEL Reviewed; 268 AA.
AC Q18054;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Helix-loop-helix protein 25 {ECO:0000312|WormBase:C17C3.7};
GN Name=hlh-25 {ECO:0000312|WormBase:C17C3.7};
GN ORFNames=C17C3.7 {ECO:0000312|WormBase:C17C3.7};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=26438299; DOI=10.1534/g3.115.019463;
RA Chou H.T., Vazquez R.G., Wang K., Campbell R., Milledge G.Z.,
RA Walthall W.W., Johnson C.M.;
RT "HES-Mediated Repression of Pten in Caenorhabditis elegans.";
RL G3 (Bethesda) 5:2619-2628(2015).
CC -!- FUNCTION: Probable transcription factor (PubMed:26438299). Modulates
CC lifespan and also recovery from the developmentally arrested larval
CC state known as dauer, perhaps acting upstream of phosphatase PTEN/daf-
CC 18 (PubMed:26438299). Regulates expression of genes involved in cell
CC division, cell-cycle regulation, and sexual reproduction, including
CC daf-18 (PubMed:26438299). {ECO:0000269|PubMed:26438299}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DEVELOPMENTAL STAGE: Expressed postembryonically at all larval and
CC adult stages, including the developmentally arrested larval state known
CC as dauer stage (PubMed:26438299). Expressed in neurons of the head and
CC tail, including the motor neurons VD1, DA3, and DA7 (PubMed:26438299).
CC Also expressed in the anal depressor muscle, anterior and posterior
CC intestinal cells, and the head and body wall muscles (PubMed:26438299).
CC Expressed in the PDE sensory neuron pairs and rare, transient
CC expression seen in migrating distal tip cells of larval L3/L4 stages
CC (PubMed:26438299). {ECO:0000269|PubMed:26438299}.
CC -!- DISRUPTION PHENOTYPE: Hermaphrodites produce fewer live progeny at 20
CC degrees Celsius than wild-type, and also laying more unfertilized
CC oocytes throughout the egg-laying period (PubMed:26438299). Abnormal
CC gonad morphology (PubMed:26438299). Delayed recovery from the
CC developmentally arrested larval state known as dauer (PubMed:26438299).
CC Significantly longer lifespan (PubMed:26438299). Decreased movement
CC (PubMed:26438299). RNAi-mediated knockdown causes an increase in daf-18
CC transcripts (PubMed:26438299). {ECO:0000269|PubMed:26438299}.
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DR EMBL; BX284602; CCD64851.1; -; Genomic_DNA.
DR RefSeq; NP_495063.2; NM_062662.2.
DR AlphaFoldDB; Q18054; -.
DR SMR; Q18054; -.
DR IntAct; Q18054; 11.
DR STRING; 6239.C17C3.7; -.
DR PaxDb; Q18054; -.
DR EnsemblMetazoa; C17C3.7.1; C17C3.7.1; WBGene00001964.
DR GeneID; 182717; -.
DR KEGG; cel:CELE_C17C3.7; -.
DR UCSC; C17C3.7; c. elegans.
DR CTD; 182717; -.
DR WormBase; C17C3.7; CE42919; WBGene00001964; hlh-25.
DR eggNOG; ENOG502TKHE; Eukaryota.
DR GeneTree; ENSGT00970000196840; -.
DR HOGENOM; CLU_1162055_0_0_1; -.
DR InParanoid; Q18054; -.
DR OrthoDB; 1891977at2759; -.
DR PhylomeDB; Q18054; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001964; Expressed in embryo and 1 other tissue.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..268
FT /note="Helix-loop-helix protein 25"
FT /id="PRO_0000455601"
FT DOMAIN 92..149
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..105
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 106..149
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT COMPBIAS 1..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 268 AA; 31156 MW; D65BB527AC3D38BE CRC64;
MPKVIQSSMS DYRSVPYNQT PKSASERKRR NITNELINEC KTIVQKSEEE HISQEVVLFR
IVKLVTGVNL ESNFSSNDLS ESTRRKFDTE SERRKVKTER EKIRRKKQDD CYAELKFFIL
NKQMGSYEQR LKLERITILE IIIDYIKHNS DLLYPETIPQ ILPLLAGKST ATCENKENEK
PKTRMEVKDL FPRLTFQEVQ ESPTSTSPLL TFPCIPMIPT TQFNVLSNYN TVPSIFSAPL
RFILPSLQIL TPETSDEEEN EETLDIIN