HLH26_CAEEL
ID HLH26_CAEEL Reviewed; 210 AA.
AC Q18053;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Helix-loop-helix protein 26 {ECO:0000303|PubMed:19632181};
GN Name=hlh-26 {ECO:0000312|WormBase:C17C3.8};
GN ORFNames=C17C3.8 {ECO:0000312|WormBase:C17C3.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15935776; DOI=10.1016/j.devcel.2005.03.012;
RA Neves A., Priess J.R.;
RT "The REF-1 family of bHLH transcription factors pattern C. elegans embryos
RT through Notch-dependent and Notch-independent pathways.";
RL Dev. Cell 8:867-879(2005).
RN [3] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=18003741; DOI=10.1242/dev.008680;
RA Neves A., English K., Priess J.R.;
RT "Notch-GATA synergy promotes endoderm-specific expression of ref-1 in C.
RT elegans.";
RL Development 134:4459-4468(2007).
RN [4] {ECO:0000305}
RP FUNCTION, DNA-BINDING, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=19632181; DOI=10.1016/j.cell.2009.04.058;
RA Grove C.A., De Masi F., Barrasa M.I., Newburger D.E., Alkema M.J.,
RA Bulyk M.L., Walhout A.J.M.;
RT "A multiparameter network reveals extensive divergence between C. elegans
RT bHLH transcription factors.";
RL Cell 138:314-327(2009).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=35263319; DOI=10.1371/journal.pbio.3001581;
RA Sang Y., Ren J., Aballay A.;
RT "The transcription factor HLH-26 controls probiotic-mediated protection
RT against intestinal infection through up-regulation of the Wnt/BAR-1
RT pathway.";
RL PLoS Biol. 20:e3001581-e3001581(2022).
CC -!- FUNCTION: As a homodimer binds DNA via the E-box sequence 5'-CACGTG-3'
CC (PubMed:19632181). Represses lag-2 transcription during embryogenesis
CC via Notch signaling, in an unc-37-dependent manner (PubMed:15935776).
CC Also represses tbx-37 independent of Notch signaling (PubMed:15935776).
CC In the intestine, plays a role in probiotic-mediated protection against
CC infections by pathogens such as S.enterica (PubMed:35263319). This is
CC most likely by positively regulating the expression of genes such as
CC bar-1 upon exposure to probiotic bacteria such as the E.faecium
CC (PubMed:35263319). {ECO:0000269|PubMed:15935776,
CC ECO:0000269|PubMed:19632181, ECO:0000269|PubMed:35263319}.
CC -!- SUBUNIT: Homodimer; binds to DNA as a homodimer.
CC {ECO:0000269|PubMed:19632181}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000305|PubMed:19632181}.
CC -!- TISSUE SPECIFICITY: Expressed in intestinal cells (at protein level).
CC {ECO:0000269|PubMed:35263319}.
CC -!- DEVELOPMENTAL STAGE: Expressed in AB cell descendants in embryos.
CC {ECO:0000269|PubMed:15935776, ECO:0000269|PubMed:18003741}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in the intestine
CC decreases the E.faecium-mediated protection against S. enterica
CC infection (PubMed:35263319). RNAi-mediated knockdown reduces the
CC expression of genes including bar-1 following E.faecium infection
CC (PubMed:35263319). RNAi-mediated knockdown in the germline or the
CC nervous system does not affect E.faecium-mediated protection against
CC S.enterica infection (PubMed:35263319). {ECO:0000269|PubMed:35263319}.
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DR EMBL; BX284602; CCD64839.1; -; Genomic_DNA.
DR PIR; T15528; T15528.
DR RefSeq; NP_495062.1; NM_062661.3.
DR AlphaFoldDB; Q18053; -.
DR BioGRID; 39281; 33.
DR IntAct; Q18053; 32.
DR STRING; 6239.C17C3.8; -.
DR PaxDb; Q18053; -.
DR EnsemblMetazoa; C17C3.8.1; C17C3.8.1; WBGene00001965.
DR GeneID; 173939; -.
DR KEGG; cel:CELE_C17C3.8; -.
DR UCSC; C17C3.8; c. elegans.
DR CTD; 173939; -.
DR WormBase; C17C3.8; CE04028; WBGene00001965; hlh-26.
DR eggNOG; ENOG502TFEF; Eukaryota.
DR GeneTree; ENSGT00970000196840; -.
DR HOGENOM; CLU_1311103_0_0_1; -.
DR InParanoid; Q18053; -.
DR OMA; RRDDTND; -.
DR PhylomeDB; Q18053; -.
DR SignaLink; Q18053; -.
DR PRO; PR:Q18053; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001965; Expressed in embryo and 1 other tissue.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 2.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Notch signaling pathway; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..210
FT /note="Helix-loop-helix protein 26"
FT /id="PRO_0000388708"
FT DOMAIN 14..65
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..33
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 210 AA; 23874 MW; 539C6E789C7350A6 CRC64;
MSSSPTSSSS GSPSSHGHRS ETEKQRRDDT NDLLNEFKKI VQKSESEKLS KEEVLFRIVK
LLSGIQLHHE SFSTSPGPIR SIKKIKSDRE QVRRNKRVAA YRELRKFIAL NNLSSSEEID
KMENLKVLEI IFEVIRGKSI TCTVPCLPFP ILPFLPVFPV YSPLVFNSYN QFSLYPPHMP
TVQIPIVPSS SFEIDALEIE ENEKDIDIVG
