HLH4_CAEEL
ID HLH4_CAEEL Reviewed; 205 AA.
AC P34555;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Helix-loop-helix protein 4;
GN Name=hlh-4; ORFNames=T05G5.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=27487365; DOI=10.1371/journal.pgen.1006237;
RA Gruner M., Grubbs J., McDonagh A., Valdes D., Winbush A.,
RA van der Linden A.M.;
RT "Cell-Autonomous and Non-Cell-Autonomous Regulation of a Feeding State-
RT Dependent Chemoreceptor Gene via MEF-2 and bHLH Transcription Factors.";
RL PLoS Genet. 12:E1006237-E1006237(2016).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=29672507; DOI=10.1371/journal.pbio.2004979;
RA Masoudi N., Tavazoie S., Glenwinkel L., Ryu L., Kim K., Hobert O.;
RT "Unconventional function of an Achaete-Scute homolog as a terminal selector
RT of nociceptive neuron identity.";
RL PLoS Biol. 16:E2004979-E2004979(2018).
CC -!- FUNCTION: Acts as a transcriptional regulator (PubMed:27487365,
CC PubMed:29672507). May mediate transcriptional activation by binding to
CC the E-box motif 5'-CANNTG-3' (PubMed:29672507). Required for the
CC correct morphology, terminal identity and function of the ADL sensory
CC neurons by controlling the expression of the ADL-specific gene
CC repertoire, including chemoreceptor encoding genes, ion channel
CC encoding genes, neuropeptides and the neurotransmitter eat-4
CC (PubMed:29672507). Regulates the expression of the srh-234
CC chemoreceptor encoding gene in the ADL neurons under feeding conditions
CC (PubMed:27487365). Plays a role in the chemorepulsive response toward
CC ascaroside pheromones mediated by the ADL sensory neurons
CC (PubMed:29672507). {ECO:0000269|PubMed:27487365,
CC ECO:0000269|PubMed:29672507}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in the ADL sensory neurons.
CC {ECO:0000269|PubMed:27487365, ECO:0000269|PubMed:29672507}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the ADL precursor cells in the
CC precomma stage embryo (PubMed:29672507). Expressed during
CC embryogenesis, larval stages and in adult animals (PubMed:27487365,
CC PubMed:29672507). {ECO:0000269|PubMed:27487365,
CC ECO:0000269|PubMed:29672507}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in a rrf-3 RNAi
CC hypersensitive mutant background leads to decreased expression of srh-
CC 234 in the cell body of ADL sensory neurons (PubMed:27487365).
CC {ECO:0000269|PubMed:27487365}.
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DR EMBL; Z27079; CAA81589.1; -; Genomic_DNA.
DR PIR; S41002; S41002.
DR RefSeq; NP_499150.1; NM_066749.1.
DR AlphaFoldDB; P34555; -.
DR SMR; P34555; -.
DR BioGRID; 41567; 3.
DR IntAct; P34555; 3.
DR STRING; 6239.T05G5.2; -.
DR PaxDb; P34555; -.
DR EnsemblMetazoa; T05G5.2.1; T05G5.2.1; WBGene00001951.
DR EnsemblMetazoa; T05G5.2.2; T05G5.2.2; WBGene00001951.
DR GeneID; 176372; -.
DR UCSC; T05G5.2; c. elegans.
DR CTD; 176372; -.
DR WormBase; T05G5.2; CE00314; WBGene00001951; hlh-4.
DR eggNOG; KOG4029; Eukaryota.
DR HOGENOM; CLU_1349975_0_0_1; -.
DR InParanoid; P34555; -.
DR OMA; QFTKRVS; -.
DR OrthoDB; 1500285at2759; -.
DR PRO; PR:P34555; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00001951; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:WormBase.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IPI:WormBase.
DR GO; GO:0043425; F:bHLH transcription factor binding; IPI:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:WormBase.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:WormBase.
DR GO; GO:0032094; P:response to food; IMP:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..205
FT /note="Helix-loop-helix protein 4"
FT /id="PRO_0000127226"
FT DOMAIN 3..56
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 3..16
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 17..56
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 205 AA; 22692 MW; 577E59E04268FA1C CRC64;
MPMVVAKRNA RERTRVHTVN QAFLVLKQHL PSLRQFTKRV SKLRILNAAI TYIDTLLKLI
QSSEAVPQSV ISATLGPIVP TPVRAVHKIS KPDTVISQPI RPLAPVLPRH ETYLPAPIAA
TCAPDHSLVD YRSTFASSLA PPVPMQMPSV FSPQPTFPYM KSLLDYPTYF YQPSTAPPPP
PAPTAPQSHL IVNNQLVPMP SYQCF
