ID   HLH6_CAEEL              Reviewed;         268 AA.
AC   Q10007;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Helix-loop-helix protein 6;
GN   Name=hlh-6; ORFNames=T15H9.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=17049341; DOI=10.1016/j.ydbio.2006.09.036;
RA   Raharjo I., Gaudet J.;
RT   "Gland-specific expression of C. elegans hlh-6 requires the combinatorial
RT   action of three distinct promoter elements.";
RL   Dev. Biol. 302:295-308(2007).
RN   [3]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=18927627; DOI=10.1371/journal.pgen.1000222;
RA   Smit R.B., Schnabel R., Gaudet J.;
RT   "The HLH-6 transcription factor regulates C. elegans pharyngeal gland
RT   development and function.";
RL   PLoS Genet. 4:E1000222-E1000222(2008).
CC   -!- FUNCTION: Transcription factor that regulates the development of the g2
CC       pharyngeal gland cells and pharyngeal gland function and thereby is
CC       required for feeding (PubMed:18927627). Required for the expression of
CC       a number of genes in the pharyngeal gland, possibly by binding to the E
CC       box motif (5'-CANNTG-3') in the promoter region of these genes
CC       (PubMed:18927627). Positively regulates the expression of genes
CC       encoding mucin-like proteins, which lubricate the pharyngeal tract to
CC       ensure efficient passage of the bacterial food source
CC       (PubMed:18927627). Exhibits pharyngeal gland-specific positive
CC       autoregulation activity (PubMed:17049341).
CC       {ECO:0000269|PubMed:17049341, ECO:0000269|PubMed:18927627}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Expressed in the gland cells of the pharynx and
CC       weakly in the pharyngeal neuron. {ECO:0000269|PubMed:17049341,
CC       ECO:0000269|PubMed:18927627}.
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DR   EMBL; Z47356; CAA87416.1; -; Genomic_DNA.
DR   PIR; T24940; T24940.
DR   RefSeq; NP_496070.1; NM_063669.1.
DR   AlphaFoldDB; Q10007; -.
DR   SMR; Q10007; -.
DR   BioGRID; 53201; 1.
DR   IntAct; Q10007; 1.
DR   STRING; 6239.T15H9.3; -.
DR   PaxDb; Q10007; -.
DR   EnsemblMetazoa; T15H9.3.1; T15H9.3.1; WBGene00001952.
DR   GeneID; 188539; -.
DR   KEGG; cel:CELE_T15H9.3; -.
DR   UCSC; T15H9.3; c. elegans.
DR   CTD; 188539; -.
DR   WormBase; T15H9.3; CE01666; WBGene00001952; hlh-6.
DR   eggNOG; KOG4029; Eukaryota.
DR   HOGENOM; CLU_109959_0_0_1; -.
DR   InParanoid; Q10007; -.
DR   OMA; FLTMFPV; -.
DR   OrthoDB; 1626551at2759; -.
DR   PRO; PR:Q10007; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00001952; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR   GO; GO:0042995; C:cell projection; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:WormBase.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1905905; P:pharyngeal gland morphogenesis; IMP:UniProtKB.
DR   GO; GO:0060465; P:pharynx development; IMP:WormBase.
DR   GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; IMP:UniProtKB.
DR   GO; GO:1904000; P:positive regulation of eating behavior; IMP:UniProtKB.
DR   GO; GO:0110043; P:positive regulation of pharynx morphogenesis; IMP:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR015660; MASH1/Ascl1a-like.
DR   PANTHER; PTHR13935; PTHR13935; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..268
FT                   /note="Helix-loop-helix protein 6"
FT                   /id="PRO_0000127227"
FT   DOMAIN          173..225
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          117..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   268 AA;  30825 MW;  8C7A42EDDAECA785 CRC64;
     MSISQNNFLT MFPVTYTFEN GVYSTIINQN TIQTPIPNPI QHHIQNHIQT SIPNTNLLLE
     NVQTDVQKLM VPLIDQQFHI PTSTPLQLAP IPTQIQSQLQ PQISQIPIHN QPQIQIQSQV
     QPQLPTQSQP KPSSKASLDT SSNAFKKYVN PFAPEATVPL PVELEDQYGP YSSSVWKRNE
     RERCRVRNVN DGYERLRKHL PVHFDEKRIS KVDTLRLAIR YIKHLDNLLR SELHQYNCKC
     FNGFQEESEG NILIDISTFN FNSSNNAM