HLP_ARATH
ID HLP_ARATH Reviewed; 173 AA.
AC Q93Z17; F4KG27; Q8RWH1; Q9FNK6;
DT 11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=50S ribosomal protein HLP, mitochondrial;
DE AltName: Full=Protein HUELLENLOS PARALOG;
DE Flags: Precursor;
GN Name=HLP; OrderedLocusNames=At5g46160; ORFNames=MCL19.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=11752383; DOI=10.2307/3871530;
RA Skinner D.J., Baker S.C., Meister R.J., Broadhvest J., Schneitz K.,
RA Gasser C.S.;
RT "The Arabidopsis HUELLENLOS gene, which is essential for normal ovule
RT development, encodes a mitochondrial ribosomal protein.";
RL Plant Cell 13:2719-2730(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Binds to 23S rRNA in mitochondrion. {ECO:0000250}.
CC -!- SUBUNIT: Part of the mitochondrial 50S ribosomal subunit.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:11752383}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93Z17-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93Z17-2; Sequence=VSP_054900;
CC -!- TISSUE SPECIFICITY: Mostly expressed in leaves and inflorescences,
CC including floral organs and meristems, and, to a lower extent, in
CC pistils. {ECO:0000269|PubMed:11752383}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uL14 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08261.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF402993; AAL60452.1; -; mRNA.
DR EMBL; AB006698; BAB08261.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95347.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95348.1; -; Genomic_DNA.
DR EMBL; AY058844; AAL24232.1; -; mRNA.
DR EMBL; AY093096; AAM13095.1; -; mRNA.
DR EMBL; AY128773; AAM91173.1; -; mRNA.
DR RefSeq; NP_199428.3; NM_123985.3. [Q93Z17-2]
DR RefSeq; NP_851140.1; NM_180809.3. [Q93Z17-1]
DR PDB; 6XYW; EM; 3.86 A; Ak=1-173.
DR PDBsum; 6XYW; -.
DR AlphaFoldDB; Q93Z17; -.
DR SMR; Q93Z17; -.
DR IntAct; Q93Z17; 1.
DR STRING; 3702.AT5G46160.1; -.
DR PaxDb; Q93Z17; -.
DR PRIDE; Q93Z17; -.
DR ProteomicsDB; 228743; -. [Q93Z17-1]
DR EnsemblPlants; AT5G46160.1; AT5G46160.1; AT5G46160. [Q93Z17-1]
DR EnsemblPlants; AT5G46160.2; AT5G46160.2; AT5G46160. [Q93Z17-2]
DR GeneID; 834658; -.
DR Gramene; AT5G46160.1; AT5G46160.1; AT5G46160. [Q93Z17-1]
DR Gramene; AT5G46160.2; AT5G46160.2; AT5G46160. [Q93Z17-2]
DR KEGG; ath:AT5G46160; -.
DR Araport; AT5G46160; -.
DR TAIR; locus:2161368; AT5G46160.
DR eggNOG; KOG0901; Eukaryota.
DR InParanoid; Q93Z17; -.
DR OMA; IRSFIQM; -.
DR PhylomeDB; Q93Z17; -.
DR PRO; PR:Q93Z17; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93Z17; baseline and differential.
DR Genevisible; Q93Z17; AT.
DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0070180; F:large ribosomal subunit rRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 2.40.150.20; -; 1.
DR HAMAP; MF_01367; Ribosomal_L14; 1.
DR InterPro; IPR036853; Ribosomal_L14_sf.
DR InterPro; IPR000218; Ribosomal_L14P.
DR InterPro; IPR005745; Ribosomal_L14P_bac-type.
DR InterPro; IPR019972; Ribosomal_L14P_CS.
DR PANTHER; PTHR11761; PTHR11761; 1.
DR Pfam; PF00238; Ribosomal_L14; 1.
DR SMART; SM01374; Ribosomal_L14; 1.
DR SUPFAM; SSF50193; SSF50193; 1.
DR TIGRFAMs; TIGR01067; rplN_bact; 1.
DR PROSITE; PS00049; RIBOSOMAL_L14; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Mitochondrion; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding;
KW Transit peptide.
FT TRANSIT 1..61
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 62..173
FT /note="50S ribosomal protein HLP, mitochondrial"
FT /id="PRO_0000429332"
FT VAR_SEQ 40
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_054900"
FT CONFLICT 23
FT /note="G -> V (in Ref. 4; AAM13095/AAM91173)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 173 AA; 18569 MW; 7F3618C69AE02895 CRC64;
MAAAFASRLT RGGRSLLGGL NNGGSMNSSN GMMNESILSQ QQQRRTFIQM GTVLKVVDNS
GAKKVMCIQA LKGKKGARLG DTIVASVKEA MPNGKVKKGA VVYGVVVRAA MQRGRVDGSE
VRFDDNAVVL VDSKDKNTKT DRQPIGTRVF GPVPHELRKK KHLKILALAQ HVA
