HLY1_VIBPA
ID HLY1_VIBPA Reviewed; 189 AA.
AC P19249; P07624;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2003, sequence version 2.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Thermostable direct hemolysin 1;
DE AltName: Full=Kanagawa phenomenon-associated hemolysin;
DE Flags: Precursor;
GN Name=tdh1; Synonyms=tdh; OrderedLocusNames=VPA1378;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=T4750;
RX PubMed=3988703; DOI=10.1128/jb.162.2.558-564.1985;
RA Nishibuchi M., Kaper J.B.;
RT "Nucleotide sequence of the thermostable direct hemolysin gene of Vibrio
RT parahaemolyticus.";
RL J. Bacteriol. 162:558-564(1985).
RN [2]
RP PROTEIN SEQUENCE, AND DISULFIDE BOND.
RX PubMed=3571196; DOI=10.1093/oxfordjournals.jbchem.a121882;
RA Tsunasawa S., Sugihara A., Masaki T., Sakiyama F., Takeda Y., Miwatani T.,
RA Narita K.;
RT "Amino acid sequence of thermostable direct hemolysin produced by Vibrio
RT parahaemolyticus.";
RL J. Biochem. 101:111-121(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=T4750;
RX PubMed=2227429; DOI=10.1016/0378-1119(90)90129-f;
RA Iida T., Yamamoto K.;
RT "Cloning and expression of two genes encoding highly homologous hemolysins
RT from a Kanagawa phenomenon-positive Vibrio parahaemolyticus T4750 strain.";
RL Gene 93:9-15(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2319944; DOI=10.1111/j.1365-2958.1990.tb02017.x;
RA Nishibuchi M., Kaper J.B.;
RT "Duplication and variation of the thermostable direct haemolysin (tdh) gene
RT in Vibrio parahaemolyticus.";
RL Mol. Microbiol. 4:87-99(1990).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
CC -!- FUNCTION: Bacterial hemolysins are exotoxins that attack blood cell
CC membranes and cause cell rupture by mechanisms not clearly defined.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the TDH hemolysin family. {ECO:0000305}.
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DR EMBL; M10069; AAA27569.1; -; Genomic_DNA.
DR EMBL; D90101; BAA14132.1; -; Genomic_DNA.
DR EMBL; X54340; CAA38228.1; -; Genomic_DNA.
DR EMBL; BA000032; BAC62721.1; -; Genomic_DNA.
DR PIR; A25100; LEVCTP.
DR RefSeq; NP_800888.1; NC_004605.1.
DR RefSeq; WP_005478284.1; NC_004605.1.
DR AlphaFoldDB; P19249; -.
DR SMR; P19249; -.
DR STRING; 223926.28809746; -.
DR EnsemblBacteria; BAC62721; BAC62721; BAC62721.
DR GeneID; 1192074; -.
DR KEGG; vpa:VPA1378; -.
DR PATRIC; fig|223926.6.peg.4303; -.
DR HOGENOM; CLU_1495597_0_0_6; -.
DR Proteomes; UP000002493; Chromosome 2.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0019836; P:hemolysis by symbiont of host erythrocytes; IEA:InterPro.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR Gene3D; 2.60.270.30; -; 1.
DR InterPro; IPR038689; TDH_sf.
DR InterPro; IPR005015; Thermostable_hemolysn_vibrio.
DR Pfam; PF03347; TDH; 1.
PE 1: Evidence at protein level;
KW Cytolysis; Direct protein sequencing; Disulfide bond; Hemolysis;
KW Reference proteome; Signal; Toxin; Virulence.
FT SIGNAL 1..24
FT CHAIN 25..189
FT /note="Thermostable direct hemolysin 1"
FT /evidence="ECO:0000269|PubMed:3571196"
FT /id="PRO_0000013361"
FT DISULFID 175..185
FT /evidence="ECO:0000269|PubMed:3571196"
FT CONFLICT 52
FT /note="Q -> N (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="K -> E (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 74
FT /note="E -> K (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="S -> H (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="G -> D (in Ref. 2; AA sequence and 3; BAA14132)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="N -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="N -> S (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 142..143
FT /note="EG -> QD (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 189 AA; 21295 MW; C16FB313C5063B01 CRC64;
MKHQYFAKKS FLFISMLAAF KTSAFELPSV PFPAPGSDEI LFVVRDTTFN TQAPVNVKVS
DFWTNRNVKR KPYEDVYGQS VFTTSGTKWL TSYMTVNIND KDYTMAAVSG YKSGHSAVFV
KSGQVQLQHS YNSVANFVGE DEGSIPSKMY LDETPEYFVN VEAYESGSGN ILVMCISNKE
SFFECKHQQ
