ID   HLY2_ACTPL              Reviewed;         194 AA.
AC   P46028;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=21 kDa hemolysin;
DE   Flags: Precursor;
GN   Name=hly;
OS   Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Actinobacillus.
OX   NCBI_TaxID=715;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K17 / Serotype 5;
RX   PubMed=8461417; DOI=10.1292/jvms.55.173;
RA   Ito H., Uchida I., Sekizaki T., Terakado N.;
RT   "A cryptic DNA sequence, isolated from Actinobacillus pleuropneumoniae,
RT   confers a hemolytic activity upon Escherichia coli K12 strains.";
RL   J. Vet. Med. Sci. 55:173-175(1993).
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
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DR   EMBL; S57691; AAB26060.1; -; Genomic_DNA.
DR   AlphaFoldDB; P46028; -.
DR   SMR; P46028; -.
DR   STRING; 228399.appser1_14890; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR007055; BON_dom.
DR   InterPro; IPR014004; Transpt-assoc_nodulatn_dom_bac.
DR   Pfam; PF04972; BON; 2.
DR   SMART; SM00749; BON; 2.
DR   PROSITE; PS50914; BON; 2.
PE   3: Inferred from homology;
KW   Cytolysis; Hemolysis; Periplasm; Repeat; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..194
FT                   /note="21 kDa hemolysin"
FT                   /id="PRO_0000013913"
FT   DOMAIN          49..118
FT                   /note="BON 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00229"
FT   DOMAIN          127..194
FT                   /note="BON 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00229"
SQ   SEQUENCE   194 AA;  21219 MW;  6C9E6A015B6BC32E CRC64;
     MRTRSRSTVR PLWPPPSPAR FATWSHYRLR DHGDHTRPVD PRSAGTQVDD EVLEERVAYN
     LSKDAQLKEE ARINVVAYNG KVLLIGQAPT MSASESAKNL AAGAEGVTEI YNEIRTGEKI
     GVGQISIDSW ITTAIKSKLL ANSEVKATEV KVITENGEVF LIGKLSPAQA DAAAEVARNV
     RGVNKVIKVI NYVQ