HLY2_VIBPA
ID HLY2_VIBPA Reviewed; 189 AA.
AC P19250; P07624; Q9RIH5;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2003, sequence version 2.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Thermostable direct hemolysin 2;
DE AltName: Full=Kanagawa phenomenon-associated hemolysin;
DE Flags: Precursor;
GN Name=tdh2; Synonyms=tdh, trh; OrderedLocusNames=VPA1314;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=T4750;
RX PubMed=2227429; DOI=10.1016/0378-1119(90)90129-f;
RA Iida T., Yamamoto K.;
RT "Cloning and expression of two genes encoding highly homologous hemolysins
RT from a Kanagawa phenomenon-positive Vibrio parahaemolyticus T4750 strain.";
RL Gene 93:9-15(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=WP1;
RX PubMed=2319944; DOI=10.1111/j.1365-2958.1990.tb02017.x;
RA Nishibuchi M., Kaper J.B.;
RT "Duplication and variation of the thermostable direct haemolysin (tdh) gene
RT in Vibrio parahaemolyticus.";
RL Mol. Microbiol. 4:87-99(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
RN [4]
RP PROTEIN SEQUENCE OF 25-189.
RX PubMed=3571196; DOI=10.1093/oxfordjournals.jbchem.a121882;
RA Tsunasawa S., Sugihara A., Masaki T., Sakiyama F., Takeda Y., Miwatani T.,
RA Narita K.;
RT "Amino acid sequence of thermostable direct hemolysin produced by Vibrio
RT parahaemolyticus.";
RL J. Biochem. 101:111-121(1987).
CC -!- FUNCTION: Bacterial hemolysins are exotoxins that attack blood cell
CC membranes and cause cell rupture by mechanisms not clearly defined.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the TDH hemolysin family. {ECO:0000305}.
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DR EMBL; D90100; BAA14131.1; -; Genomic_DNA.
DR EMBL; X54341; CAA38229.2; -; Genomic_DNA.
DR EMBL; BA000032; BAC62657.1; -; Genomic_DNA.
DR PIR; JW0035; JW0035.
DR RefSeq; NP_800824.1; NC_004605.1.
DR RefSeq; WP_005463268.1; NC_004605.1.
DR PDB; 3A57; X-ray; 1.50 A; A=25-189.
DR PDBsum; 3A57; -.
DR AlphaFoldDB; P19250; -.
DR SMR; P19250; -.
DR STRING; 223926.28809682; -.
DR EnsemblBacteria; BAC62657; BAC62657; BAC62657.
DR GeneID; 1192010; -.
DR KEGG; vpa:VPA1314; -.
DR PATRIC; fig|223926.6.peg.4238; -.
DR HOGENOM; CLU_1495597_0_0_6; -.
DR EvolutionaryTrace; P19250; -.
DR Proteomes; UP000002493; Chromosome 2.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0019836; P:hemolysis by symbiont of host erythrocytes; IEA:InterPro.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR DisProt; DP00668; -.
DR Gene3D; 2.60.270.30; -; 1.
DR InterPro; IPR038689; TDH_sf.
DR InterPro; IPR005015; Thermostable_hemolysn_vibrio.
DR Pfam; PF03347; TDH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytolysis; Direct protein sequencing; Disulfide bond;
KW Hemolysis; Reference proteome; Signal; Toxin; Virulence.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:3571196"
FT CHAIN 25..189
FT /note="Thermostable direct hemolysin 2"
FT /id="PRO_0000013362"
FT DISULFID 175..185
FT CONFLICT 136
FT /note="N -> S (in Ref. 1; BAA14131 and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="E -> Q (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 37..46
FT /evidence="ECO:0007829|PDB:3A57"
FT HELIX 47..50
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 51..53
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 57..70
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 77..98
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 101..112
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 115..124
FT /evidence="ECO:0007829|PDB:3A57"
FT HELIX 131..138
FT /evidence="ECO:0007829|PDB:3A57"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 145..153
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 158..166
FT /evidence="ECO:0007829|PDB:3A57"
FT STRAND 169..177
FT /evidence="ECO:0007829|PDB:3A57"
FT TURN 179..181
FT /evidence="ECO:0007829|PDB:3A57"
FT HELIX 182..185
FT /evidence="ECO:0007829|PDB:3A57"
SQ SEQUENCE 189 AA; 21562 MW; C3DD9AB955771921 CRC64;
MKYRYFAKKS FLFISMLAAF KTFAFELPSV PFPAPGSDEI LFVVRDTTFN TNAPVNVEVS
DFWTNRNVKR KPYKDVYGQS VFTTSGTKWL TSYMTVNIND KDYTMAAVSG YKHGHSAVFV
KSDQVQLQHS YDSVANFVGE DEDSIPSKMY LDETPEYFVN VEAYESGSGN ILVMCISNKE
SFFECKHQQ
