ID   HLY3_VIBPH              Reviewed;         189 AA.
AC   P28029;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Thermostable direct hemolysin-related;
DE   Flags: Precursor;
GN   Name=tdh3; Synonyms=tdh/I, tdhX;
OS   Vibrio parahaemolyticus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=TH012;
RX   PubMed=2016584; DOI=10.1099/00221287-137-2-253;
RA   Honda T., Abad-Lapuebla M.A., Ni Y., Yamamoto K., Miwatani T.;
RT   "Characterization of a new thermostable direct haemolysin produced by a
RT   Kanagawa-phenomenon-negative clinical isolate of Vibrio parahaemolyticus.";
RL   J. Gen. Microbiol. 137:253-259(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2319944; DOI=10.1111/j.1365-2958.1990.tb02017.x;
RA   Nishibuchi M., Kaper J.B.;
RT   "Duplication and variation of the thermostable direct haemolysin (tdh) gene
RT   in Vibrio parahaemolyticus.";
RL   Mol. Microbiol. 4:87-99(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TH3766;
RX   PubMed=8262624; DOI=10.1128/iai.62.1.166-171.1994;
RA   Xu M., Iida T., Yamamoto K., Takarada Y., Miwatani T., Honda T.;
RT   "Demonstration and characterization of simultaneous production of a
RT   thermostable direct hemolysin (TDH/I) and a TDH-related hemolysin (TRHx) by
RT   a clinically isolated Vibrio parahaemolyticus strain, TH3766.";
RL   Infect. Immun. 62:166-171(1994).
CC   -!- FUNCTION: Bacterial hemolysins are exotoxins that attack blood cell
CC       membranes and cause cell rupture by mechanisms not clearly defined.
CC   -!- SUBUNIT: Homodimer.
CC   -!- MISCELLANEOUS: This hemolysin is not associated with the Kanagawa
CC       phenomenon.
CC   -!- SIMILARITY: Belongs to the TDH hemolysin family. {ECO:0000305}.
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DR   EMBL; D90238; BAA14285.1; -; Genomic_DNA.
DR   EMBL; X54342; CAA38230.1; -; Genomic_DNA.
DR   EMBL; S67841; AAP14018.1; -; Genomic_DNA.
DR   PIR; A49748; A49748.
DR   RefSeq; WP_023650135.1; NZ_VTGL01000023.1.
DR   AlphaFoldDB; P28029; -.
DR   SMR; P28029; -.
DR   PATRIC; fig|670.322.peg.202; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0019836; P:hemolysis by symbiont of host erythrocytes; IEA:InterPro.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.270.30; -; 1.
DR   InterPro; IPR038689; TDH_sf.
DR   InterPro; IPR005015; Thermostable_hemolysn_vibrio.
DR   Pfam; PF03347; TDH; 1.
PE   1: Evidence at protein level;
KW   Cytolysis; Direct protein sequencing; Disulfide bond; Hemolysis; Signal;
KW   Toxin; Virulence.
FT   SIGNAL          1..24
FT   CHAIN           25..189
FT                   /note="Thermostable direct hemolysin-related"
FT                   /id="PRO_0000013363"
FT   DISULFID        175..185
FT                   /evidence="ECO:0000250"
FT   CONFLICT        186
FT                   /note="E -> K (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   189 AA;  21550 MW;  B281F6B4EE9A703D CRC64;
     MKYRYFAKKS FLFISMLAAF KTFAFELPSV PFPAPGSDEI LFVVRDATFN TNAPVNVKVS
     DFWTNRNVKR KPYKDVYGQS VFTTSGTKWL TSYMTVNIND KDYTMAAVSG YKRGHSAVFV
     KSDQVQLQHS YNSVANFVGE DEDSIPSKMY LDETPEYFVN VEAYESGSGN ILVMCISNKE
     SFFECEHQK