ID   HLYB_BRAHO              Reviewed;         828 AA.
AC   Q54316;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Hemolysin B;
GN   Name=tlyB;
OS   Brachyspira hyodysenteriae (Treponema hyodysenteriae).
OC   Bacteria; Spirochaetes; Brachyspirales; Brachyspiraceae; Brachyspira.
OX   NCBI_TaxID=159;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B204;
RX   PubMed=7968456; DOI=10.1006/mpat.1994.1028;
RA   Ter Huurne A.A.H.M., Muir S., van Houten M., van der Zeijst B.A.M.,
RA   Gaastra W., Kusters J.G.;
RT   "Characterization of three putative Serpulina hyodysenteriae hemolysins.";
RL   Microb. Pathog. 16:269-282(1994).
CC   -!- FUNCTION: Bacterial hemolysins are exotoxins that attack blood cell
CC       membranes and cause cell rupture by mechanisms not clearly defined.
CC   -!- SIMILARITY: Belongs to the ClpA/ClpB family. ClpC subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X73140; CAA51655.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q54316; -.
DR   SMR; Q54316; -.
DR   PRIDE; Q54316; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1780.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR019489; Clp_ATPase_C.
DR   InterPro; IPR036628; Clp_N_dom_sf.
DR   InterPro; IPR004176; Clp_R_dom.
DR   InterPro; IPR001270; ClpA/B.
DR   InterPro; IPR018368; ClpA/B_CS1.
DR   InterPro; IPR028299; ClpA/B_CS2.
DR   InterPro; IPR041546; ClpA/ClpB_AAA_lid.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001943; UVR_dom.
DR   Pfam; PF00004; AAA; 1.
DR   Pfam; PF07724; AAA_2; 1.
DR   Pfam; PF17871; AAA_lid_9; 1.
DR   Pfam; PF02861; Clp_N; 2.
DR   Pfam; PF10431; ClpB_D2-small; 1.
DR   PRINTS; PR00300; CLPPROTEASEA.
DR   SMART; SM00382; AAA; 2.
DR   SMART; SM01086; ClpB_D2-small; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF81923; SSF81923; 1.
DR   PROSITE; PS51903; CLP_R; 1.
DR   PROSITE; PS00870; CLPAB_1; 1.
DR   PROSITE; PS00871; CLPAB_2; 1.
DR   PROSITE; PS50151; UVR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Cytolysis; Hemolysis; Nucleotide-binding; Repeat;
KW   Toxin; Virulence.
FT   CHAIN           1..828
FT                   /note="Hemolysin B"
FT                   /id="PRO_0000191229"
FT   DOMAIN          4..147
FT                   /note="Clp R"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   DOMAIN          426..461
FT                   /note="UVR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00217"
FT   REGION          7..72
FT                   /note="Repeat 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          83..147
FT                   /note="Repeat 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01251"
FT   REGION          172..419
FT                   /note="I"
FT   REGION          480..671
FT                   /note="II"
FT   BINDING         217..224
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   BINDING         554..561
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   828 AA;  93311 MW;  2F7E1183000438C2 CRC64;
     MFQFHLTSKA KKVIELYAQE EAKRLNHDMV TPEHILLGLL YESEALATRV LMRLKIDLDR
     LKLELESAMV KSSTTKVFGT LPTAPRVQKL ISRSAEEARA LSHNYIGTEH LLLGLLREES
     GTAYNVLTSM GLELTILRQE ILKMLGVAGS NISSMEQTSQ EDNVKKVKTP TLDQFARDLT
     KMARDKALDR VIGRENEVMR VVQILSRRKK NNPILLGEPG VGKTAIVEGL AEKIVAADVP
     DILLKKRVLT LDLSSVVAGT KYRGEFEERI KNIVLEIKKA SNIIIFIDEL HTLIGAGGAE
     GALDAANMLK PALSRGEIQC IGATTINEYK KYIEKDGALV RRFQPINVEE PSIEDTIEIL
     NGIKGKYEEH HKVKYTDEAI NAAAVLSKRY IFERHLPDKA IDLIDEAGSR ARLLNMTRPQ
     EFKDLEKKIE ELNQQKKRVV ESQNFEDAAK IRDEITSLQE ELSKKEEKCR EEREKIETFI
     EEDDIRHVIS EITNIPIKRL LNSESKRLIG MEEELHQKVV GQKEAISSIS KAIRRSRAGL
     KTSKRPLGSF IFLGPTGVGK TALAKVLSEF MFGDSDALIR IDMSEFMEKF AVSRLIGAPP
     GYVGYEEGGG LTEKVRRKPY SLILFDEIEK AHPDVTNILL QVLEEGQLTD NFGRKVDFSN
     TIIIITSNLG ARDIVKGSSL GFNAVGSEKD ANDIKNFALE ELKQNFNPEF LNRIDDIIVF
     HTLSKEDLKD IINIMLKELN EAIKERNIVI NLSEEAKNYI IDKGFDKKYG ARSLRRAIQK
     EIEDYVSTEI LFGNIEDGDT INVDRNDGSL IFSYDKSVKT ENKELSKS