ID   HLYC_RICPR              Reviewed;         303 AA.
AC   O05961;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Hemolysin C;
GN   Name=tlyC; OrderedLocusNames=RP740;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9274032; DOI=10.1099/00221287-143-8-2783;
RA   Andersson J.O., Andersson S.G.E.;
RT   "Genomic rearrangements during evolution of the obligate intracellular
RT   parasite Rickettsia prowazekii as inferred from an analysis of 52015 bp
RT   nucleotide sequence.";
RL   Microbiology 143:2783-2795(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
RN   [3]
RP   MEDIATION OF PHAGOSOMAL ESCAPE IN SALMONELLA.
RC   STRAIN=ATCC VR-142 / Breinl;
RX   PubMed=16177343; DOI=10.1128/iai.73.10.6668-6673.2005;
RA   Whitworth T., Popov V.L., Yu X.-J., Walker D.H., Bouyer D.H.;
RT   "Expression of the Rickettsia prowazekii pld or tlyC gene in Salmonella
RT   enterica serovar Typhimurium mediates phagosomal escape.";
RL   Infect. Immun. 73:6668-6673(2005).
RN   [4]
RP   DETECTION OF HEMOLYSIN.
RC   STRAIN=ATCC VR-142 / Breinl, and Madrid E;
RX   PubMed=17301007; DOI=10.1016/j.bbapap.2007.01.001;
RA   Chao C.C., Chelius D., Zhang T., Mutumanje E., Ching W.M.;
RT   "Insight into the virulence of Rickettsia prowazekii by proteomic analysis
RT   and comparison with an avirulent strain.";
RL   Biochim. Biophys. Acta 1774:373-381(2007).
CC   -!- INDUCTION: Expressed at 30 minutes post-infection during the period
CC       that most rickettsiae escape from phagosomal vacuoles into the host
CC       cytosol.
CC   -!- MISCELLANEOUS: Transformation of Salmonella with tlyC results in the
CC       escape of transformants from endosomal vacuoles into the host cell
CC       cytosol.
CC   -!- MISCELLANEOUS: According to PubMed:17301007, the hemolysin protein was
CC       detected only in the virulent strain ATCC VR-142 / Breinl and not in
CC       the avirulent strain Madrid E, at their current detection limit of 10
CC       fmol.
CC   -!- SIMILARITY: Belongs to the UPF0053 family. Hemolysin C subfamily.
CC       {ECO:0000305}.
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DR   EMBL; Y11778; CAA72456.1; -; Genomic_DNA.
DR   EMBL; AJ235273; CAA15168.1; -; Genomic_DNA.
DR   PIR; H71633; H71633.
DR   RefSeq; NP_221092.1; NC_000963.1.
DR   RefSeq; WP_004599630.1; NC_000963.1.
DR   AlphaFoldDB; O05961; -.
DR   SMR; O05961; -.
DR   STRING; 272947.RP740; -.
DR   EnsemblBacteria; CAA15168; CAA15168; CAA15168.
DR   GeneID; 57569861; -.
DR   KEGG; rpr:RP740; -.
DR   PATRIC; fig|272947.5.peg.773; -.
DR   eggNOG; COG1253; Bacteria.
DR   HOGENOM; CLU_015237_3_1_5; -.
DR   OMA; ERRHMAL; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   CDD; cd04590; CBS_pair_CorC_HlyC_assoc; 1.
DR   Gene3D; 3.10.580.10; -; 1.
DR   Gene3D; 3.30.465.10; -; 1.
DR   InterPro; IPR000644; CBS_dom.
DR   InterPro; IPR046342; CBS_dom_sf.
DR   InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR   InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR   InterPro; IPR044751; Ion_transp-like_CBS.
DR   InterPro; IPR005170; Transptr-assoc_dom.
DR   Pfam; PF00571; CBS; 1.
DR   Pfam; PF03471; CorC_HlyC; 1.
DR   SMART; SM01091; CorC_HlyC; 1.
DR   SUPFAM; SSF54631; SSF54631; 1.
DR   SUPFAM; SSF56176; SSF56176; 1.
DR   PROSITE; PS51371; CBS; 2.
PE   2: Evidence at transcript level;
KW   CBS domain; Cytolysis; Hemolysis; Reference proteome; Repeat; Toxin;
KW   Virulence.
FT   CHAIN           1..303
FT                   /note="Hemolysin C"
FT                   /id="PRO_0000088386"
FT   DOMAIN          81..143
FT                   /note="CBS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   DOMAIN          146..203
FT                   /note="CBS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
SQ   SEQUENCE   303 AA;  34322 MW;  EB2AB7626B415940 CRC64;
     MLKSSKHEDS SSKKNQNNKL IFIVRQLFYL IKHFFSKTKT PDNFFGIIKR LKINSQKMSL
     DEFNILANLL KLEDKIVEDI MVPRSDIIAI KLTTNLEELS ESIKIAVPHT RTLIYDGTLD
     NVVGFIHIKD LFKALATKQN SPLKRLIRKH IIAAPSMKLL DLLAKMRRER THIAIVVDEY
     GGTDGLVTIE DLIEEIVGRI DDEHDQQLDS ANFKVINNST IIANARIEVE LLEEIIGEKL
     KNDDDEFDTI GGLVLTRVSS VPAIGTRIDI SENIEIEVTD ATPRSLKQVK IRLKNGLNSD
     NLT