ID   HLYD_MANHA              Reviewed;         478 AA.
AC   P16534;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Leukotoxin secretion protein D;
GN   Name=lktD;
OS   Mannheimia haemolytica (Pasteurella haemolytica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Mannheimia.
OX   NCBI_TaxID=75985;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serotype A1;
RX   PubMed=3040588; DOI=10.1128/iai.55.9.1987-1996.1987;
RA   Lo R.Y.C., Strathdee C.A., Shewen P.E.;
RT   "Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica
RT   A1.";
RL   Infect. Immun. 55:1987-1996(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serotype A1;
RX   PubMed=2914876; DOI=10.1128/jb.171.2.916-928.1989;
RA   Strathdee C.A., Lo R.Y.C.;
RT   "Cloning, nucleotide sequence, and characterization of genes encoding the
RT   secretion function of the Pasteurella haemolytica leukotoxin determinant.";
RL   J. Bacteriol. 171:916-928(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serotype A1 / PH101;
RX   PubMed=2707120; DOI=10.1089/dna.1.1989.8.15;
RA   Highlander S.K., Chidambaram M., Engler M.J., Weinstock G.M.;
RT   "DNA sequence of the Pasteurella haemolytica leukotoxin gene cluster.";
RL   DNA 8:15-28(1989).
CC   -!- FUNCTION: Involved in the transport of the Leukotoxin.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; M20730; AAA25531.1; -; Genomic_DNA.
DR   EMBL; M24197; AAA25545.1; -; Genomic_DNA.
DR   PIR; D30169; D30169.
DR   AlphaFoldDB; P16534; -.
DR   SMR; P16534; -.
DR   STRING; 75985.WC39_13380; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR039562; MFP_biotin_lipoyl_2.
DR   InterPro; IPR006144; Secretion_HlyD_CS.
DR   InterPro; IPR010129; T1SS_HlyD.
DR   Pfam; PF13533; Biotin_lipoyl_2; 1.
DR   TIGRFAMs; TIGR01843; type_I_hlyD; 1.
DR   PROSITE; PS00543; HLYD_FAMILY; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Cytolysis; Hemolysis; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..478
FT                   /note="Leukotoxin secretion protein D"
FT                   /id="PRO_0000201876"
FT   TOPO_DOM        1..59
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        81..478
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        18
FT                   /note="T -> I (in Ref. 3; AAA25545)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        47
FT                   /note="D -> E (in Ref. 3; AAA25545)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        150
FT                   /note="N -> T (in Ref. 3; AAA25545)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        236
FT                   /note="F -> L (in Ref. 3; AAA25545)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        266..267
FT                   /note="EL -> AV (in Ref. 3; AAA25545)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   478 AA;  54761 MW;  E2B8B5FF5AF988C1 CRC64;
     MKIWLSGIYE FFLRYKNTWA EVWKIRKELD HPNRKKDESE FLPAHLDLIE TPVSKKPRLI
     AYLIMLFLVV AIVLASVSKV EIVATAPGKL TFSGRSKEIK PIENAIVQEI FVKDGQFVEK
     GQLLVSLTAL GSDADIKKTM ASLSLAKLEN YRYQTLLTAI EKESLPVIDL SRTEFKDSSE
     EDRLRIKHLI EEQYTTWQKQ KTQKTLAYKR KEAEKQTIFA YVRKYEGATR IEQEKFKDFK
     ALYKQKSLSK HELLAQENKL IEAQNELAVY RSKLNELEND LLNVKEELEL ITQFFKSDVL
     EKLKQHIENE RQLRLELEKN NQRRQASMIR APVSGTVQQL KIHTIGGVVT TAETLMIIVP
     EDDVLEATAL VPNKDIGFVA AGQEVIIKVE TFPYTRYGYL TGRIKHISPD AIEQPNVGLV
     FNATIAIDRK NLTSPDGRKI DLSSGMTITA EIKTGERSVM SYLLSPLEES VTESLRER