ID   HLYS2_HYDVD             Reviewed;         244 AA.
AC   Q564A5;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Hydralysin-2;
GN   Name=Hln-2 {ECO:0000303|PubMed:15824108};
OS   Hydra viridissima (Green hydra) (Chlorohydra viridissima).
OC   Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Anthoathecata;
OC   Aplanulata; Hydridae; Hydra.
OX   NCBI_TaxID=6082;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAT66173.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TOXIC DOSE, AND MUTAGENESIS OF
RP   GLN-28; GLY-125 AND VAL-140.
RX   PubMed=15824108; DOI=10.1074/jbc.m503242200;
RA   Sher D., Fishman Y., Zhang M., Lebendiker M., Gaathon A., Mancheno J.-M.,
RA   Zlotkin E.;
RT   "Hydralysins, a new category of beta-pore-forming toxins in cnidaria.";
RL   J. Biol. Chem. 280:22847-22855(2005).
CC   -!- FUNCTION: Induces an immediate fast muscle contraction followed by
CC       flaccid paralysis when injected into blowfly larvae. Has strong
CC       cytolytic activity against insect Sf9 cells and human HeLa cells, weak
CC       activity against human JAR cells, and no activity against human HEK293
CC       cells. Binds to erythrocyte membranes and has hemolytic activity,
CC       probably mediated by oligomerization and pore formation.
CC       {ECO:0000269|PubMed:15824108, ECO:0000303|PubMed:15824108}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q86LR2}.
CC   -!- TOXIC DOSE: PD(50) is 0.103 mg/kg by injection in blowfly larvae.
CC       {ECO:0000269|PubMed:15824108}.
CC   -!- SIMILARITY: Belongs to the hydralysin family. {ECO:0000305}.
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DR   EMBL; AY655142; AAT66173.1; -; mRNA.
DR   AlphaFoldDB; Q564A5; -.
DR   SMR; Q564A5; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0044179; P:hemolysis in another organism; IDA:UniProtKB.
DR   GO; GO:0035915; P:pore formation in membrane of another organism; IDA:UniProtKB.
DR   InterPro; IPR004991; Aerolysin-like.
DR   Pfam; PF03318; ETX_MTX2; 1.
PE   1: Evidence at protein level;
KW   Cytolysis; Hemolysis; Neurotoxin; Secreted; Toxin.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..244
FT                   /note="Hydralysin-2"
FT                   /id="PRO_0000221559"
FT   MUTAGEN         28
FT                   /note="Q->E: No effect; when associated with L-140."
FT                   /evidence="ECO:0000269|PubMed:15824108"
FT   MUTAGEN         125
FT                   /note="G->E: Reduces paralytic and hemolytic activity. No
FT                   additional effect; when associated with E-28 and L-140."
FT                   /evidence="ECO:0000269|PubMed:15824108"
FT   MUTAGEN         140
FT                   /note="V->L: No effect; when associated with E-28."
FT                   /evidence="ECO:0000269|PubMed:15824108"
SQ   SEQUENCE   244 AA;  26214 MW;  1128EE3DFAA1EB81 CRC64;
     MGKELLTFSD LTWLDSSPDT VRSAFTRQYG TTPDGIALNS EGYFGYHSPP ITEQYGRPCY
     KQTGETKITL QDLAPPTDAI LGNSIARNRG DSPITLTVSV EGKWSDSTSW STSTETGVKM
     SSEFGVEGAF KMGGEFSLTV SVGKSGSSSV EKTSTSSVQV TVPPRSKVVV SMVGIMKKEK
     VFFEVPVTVD GSFGANFPST VQGHYFWFMD ARSCLNKTSG VIKGTIDHAN VFDVSVEVGP
     SEPL