ID   HLYT_GRIHO              Reviewed;         165 AA.
AC   P14711;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Hemolysin, heat labile;
OS   Grimontia hollisae (Vibrio hollisae).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Grimontia.
OX   NCBI_TaxID=673;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=RIMD 2221001;
RX   PubMed=2592352; DOI=10.1128/jb.171.12.6859-6861.1989;
RA   Yoh M., Honda T., Miwatani T., Tsunasawa S., Sakiyama F.;
RT   "Comparative amino acid sequence analysis of hemolysins produced by Vibrio
RT   hollisae and Vibrio parahaemolyticus.";
RL   J. Bacteriol. 171:6859-6861(1989).
CC   -!- FUNCTION: Bacterial hemolysins are exotoxins that attack blood cell
CC       membranes and cause cell rupture by mechanisms not clearly defined.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the TDH hemolysin family. {ECO:0000305}.
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DR   PIR; A43728; A43728.
DR   PDB; 4WX3; X-ray; 1.70 A; A/B/C/D=1-165.
DR   PDB; 4WX5; X-ray; 2.30 A; A/B/C/D=1-165.
DR   PDBsum; 4WX3; -.
DR   PDBsum; 4WX5; -.
DR   AlphaFoldDB; P14711; -.
DR   SMR; P14711; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0019836; P:hemolysis by symbiont of host erythrocytes; IEA:InterPro.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.270.30; -; 1.
DR   InterPro; IPR038689; TDH_sf.
DR   InterPro; IPR005015; Thermostable_hemolysn_vibrio.
DR   Pfam; PF03347; TDH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytolysis; Direct protein sequencing; Disulfide bond;
KW   Hemolysis; Toxin; Virulence.
FT   CHAIN           1..165
FT                   /note="Hemolysin, heat labile"
FT                   /id="PRO_0000196247"
FT   DISULFID        151..161
FT   STRAND          13..22
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   TURN            23..26
FT                   /evidence="ECO:0007829|PDB:4WX5"
FT   STRAND          27..29
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   STRAND          33..46
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   STRAND          53..74
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   STRAND          77..88
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   STRAND          91..100
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   HELIX           107..114
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   HELIX           118..120
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   STRAND          121..129
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   STRAND          134..142
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   STRAND          145..153
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   TURN            155..157
FT                   /evidence="ECO:0007829|PDB:4WX3"
FT   HELIX           158..161
FT                   /evidence="ECO:0007829|PDB:4WX3"
SQ   SEQUENCE   165 AA;  18630 MW;  4A50A4A45C3808B6 CRC64;
     FELPSIPFPS PGSDEILFVV RDTTFNTKEP VNVKVSDFWT NRNVKRKPYE DVYGQSVFTT
     SGSKWLTSYM TVSINNKDYT MAAVSGYKDG FSSVFVKSGQ IQLQHYYNSV ADFVGGDENS
     IPSKTYLDET PSYFVNVEAY ESGSGNILVM CISNKESYFE CEEQQ