ID   HLYU_VIBCH              Reviewed;         108 AA.
AC   P52695; Q9JMP9;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Transcriptional activator HlyU;
GN   Name=hlyU; OrderedLocusNames=VC_0678;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Classical Inaba Z17561 / Serotype O1;
RX   PubMed=8231807; DOI=10.1111/j.1365-2958.1993.tb01735.x;
RA   Williams S.G., Attridge S.R., Manning P.A.;
RT   "The transcriptional activator HlyU of Vibrio cholerae: nucleotide sequence
RT   and role in virulence gene expression.";
RL   Mol. Microbiol. 9:751-760(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=El Tor O17 / Serotype O1;
RX   PubMed=9457888; DOI=10.1128/jb.180.3.762-765.1998;
RA   Williams S.G., Carmel-Harel O., Manning P.A.;
RT   "A functional homolog of Escherichia coli NhaR in Vibrio cholerae.";
RL   J. Bacteriol. 180:762-765(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Up-regulates the expression of the hemolysin gene, hlyA, and
CC       may promote expression of other virulence determinants in vivo. It may
CC       have both positive and negative regulator activities.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-9 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; X66866; CAA47336.1; -; Genomic_DNA.
DR   EMBL; AJ002395; CAA05372.1; -; Genomic_DNA.
DR   EMBL; AE003852; AAF93843.1; -; Genomic_DNA.
DR   PIR; S37313; S37313.
DR   RefSeq; NP_230327.1; NC_002505.1.
DR   PDB; 4K2E; X-ray; 1.80 A; A/B/C/D=1-108.
DR   PDB; 4OOI; X-ray; 2.20 A; A/B/C/D=1-108.
DR   PDBsum; 4K2E; -.
DR   PDBsum; 4OOI; -.
DR   AlphaFoldDB; P52695; -.
DR   SMR; P52695; -.
DR   STRING; 243277.VC_0678; -.
DR   DNASU; 2615467; -.
DR   EnsemblBacteria; AAF93843; AAF93843; VC_0678.
DR   KEGG; vch:VC_0678; -.
DR   PATRIC; fig|243277.26.peg.650; -.
DR   eggNOG; COG0640; Bacteria.
DR   HOGENOM; CLU_097806_6_4_6; -.
DR   OMA; TIWYSIA; -.
DR   BioCyc; VCHO:VC0678-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   DisProt; DP02188; -.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01022; HTH_5; 1.
DR   PRINTS; PR00778; HTHARSR.
DR   SMART; SM00418; HTH_ARSR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50987; HTH_ARSR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..108
FT                   /note="Transcriptional activator HlyU"
FT                   /id="PRO_0000160622"
FT   DOMAIN          13..107
FT                   /note="HTH arsR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   DNA_BIND        47..66
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   HELIX           12..28
FT                   /evidence="ECO:0007829|PDB:4K2E"
FT   HELIX           31..40
FT                   /evidence="ECO:0007829|PDB:4K2E"
FT   HELIX           47..54
FT                   /evidence="ECO:0007829|PDB:4K2E"
FT   HELIX           58..70
FT                   /evidence="ECO:0007829|PDB:4K2E"
FT   STRAND          73..79
FT                   /evidence="ECO:0007829|PDB:4K2E"
FT   STRAND          82..87
FT                   /evidence="ECO:0007829|PDB:4K2E"
FT   HELIX           90..103
FT                   /evidence="ECO:0007829|PDB:4K2E"
SQ   SEQUENCE   108 AA;  12293 MW;  4FC1BE6DA960F217 CRC64;
     MPYLKGAPMN LQEMEKNSAK AVVLLKAMAN ERRLQILCML LDNELSVGEL SSRLELSQSA
     LSQHLAWLRR DGLVNTRKEA QTVFYTLSST EVKAMIELLH RLYCQANQ