ID   HLY_NATA1               Reviewed;         530 AA.
AC   P29143;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Halolysin;
DE            EC=3.4.21.-;
DE   AltName: Full=F-II;
DE   Flags: Precursor;
GN   Name=hly;
OS   Natrialba asiatica (strain ATCC 700177 / DSM 12278 / JCM 9576 / FERM
OS   P-10747 / NBRC 102637 / 172P1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales;
OC   Natrialbaceae; Natrialba.
OX   NCBI_TaxID=29540;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1732209; DOI=10.1128/jb.174.3.736-742.1992;
RA   Kamekura M., Seno Y., Holmes M.H., Dyall-Smith M.L.;
RT   "Molecular cloning and sequencing of the gene for a halophilic alkaline
RT   serine protease (halolysin) from an unidentified halophilic archaea strain
RT   (172P1) and expression of the gene in Haloferax volcanii.";
RL   J. Bacteriol. 174:736-742(1992).
RN   [2]
RP   CHARACTERIZATION, AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=2112401; DOI=10.1139/o90-048;
RA   Kamekura M., Seno Y.;
RT   "A halophilic extracellular protease from a halophilic archaebacterium
RT   strain 172 P1.";
RL   Biochem. Cell Biol. 68:352-359(1990).
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 10.7.;
CC       Temperature dependence:
CC         Thermostable. Highly active at 75-80 degrees Celsius.;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of the
CC       signal peptide cleavage has not been experimentally proven.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; D10201; BAA01049.1; -; Genomic_DNA.
DR   AlphaFoldDB; P29143; -.
DR   SMR; P29143; -.
DR   STRING; 29540.C481_09148; -.
DR   MEROPS; S08.102; -.
DR   eggNOG; arCOG00702; Archaea.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07484; Peptidases_S8_Thermitase_like; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR006311; TAT_signal.
DR   InterPro; IPR034084; Thermitase-like_dom.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Protease; Secreted; Serine protease;
KW   Signal; Zymogen.
FT   SIGNAL          1..27
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00648"
FT   PROPEP          28..119
FT                   /id="PRO_0000027036"
FT   CHAIN           120..530
FT                   /note="Halolysin"
FT                   /id="PRO_0000027037"
FT   DOMAIN          126..405
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   REGION          390..447
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        424..447
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        157
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        198
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        351
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   530 AA;  54703 MW;  FD338921AD313780 CRC64;
     MSRDTKRNIG RRSVLKATSA LGAFLGLGGV TSATPGRSRS RKKDEIVVGV SDSVSASKAT
     IDSKLPSKAT IVHTNETLGY VAVEFPSRAS TQARENFKRN VLEADDVEYA EDNATYEAIA
     TPNDPQYGQQ YAPQQVNCEA AWDVTYGDPG VTISVVDQGI QYDHEDLEGN MDGSVSNYGD
     DFVDNDGDPY PVSASENHGT HVGGIAAGGT NNATGHAGIS NCSLLSARAL GDGGGGSLTD
     IADAIQWSAD QGADVINMSL GGGGFSQTLS NACEYAYNQG SLLVAAAGNG YGNSVSYPAA
     YDTVMAVSSL DEGETLSAFS NLGPEIELAA PGGNVLSSIP WDNYDTFSGT SMASPVVAGV
     AGFTLSAHPN LSNAELRSHL QNTAVDVGLS SEEQGHGRVD AGQAVTTDPG DGGGGGDPGD
     GTCGDETNTE TAEGNISSSN PSDAYSYTLD TADPCSATVS LSGPSSADFD LYLTLDGRTP
     TTSDYDRRSY NWGSDEEISV DLSGNEELGI LVNQYSGSGS YTLTIEELGK