HM14_CAEEL
ID HM14_CAEEL Reviewed; 351 AA.
AC P20271; Q9NBV6;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 3.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=LIM/homeobox protein ceh-14 {ECO:0000305};
DE AltName: Full=Homeobox protein ceh-14 {ECO:0000312|WormBase:F46C8.5};
GN Name=ceh-14; ORFNames=F46C8.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=10774727; DOI=10.1016/s0896-6273(00)81062-4;
RA Cassata G., Kagoshima H., Andachi Y., Kohara Y., Duerrenberger M.B.,
RA Hall D.H., Buerglin T.R.;
RT "The LIM homeobox gene ceh-14 confers thermosensory function to the AFD
RT neurons in Caenorhabditis elegans.";
RL Neuron 25:587-597(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 198-239.
RX PubMed=2571091; DOI=10.1038/341239a0;
RA Buerglin T.R., Finney M., Coulson A., Ruvkun G.;
RT "Caenorhabditis elegans has scores of homoeobox-containing genes.";
RL Nature 341:239-243(1989).
RN [4]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12490565; DOI=10.1242/dev.00277;
RA Aurelio O., Boulin T., Hobert O.;
RT "Identification of spatial and temporal cues that regulate postembryonic
RT expression of axon maintenance factors in the C. elegans ventral nerve
RT cord.";
RL Development 130:599-610(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20501595; DOI=10.1242/dev.040881;
RA Van Buskirk C., Sternberg P.W.;
RT "Paired and LIM class homeodomain proteins coordinate differentiation of
RT the C. elegans ALA neuron.";
RL Development 137:2065-2074(2010).
RN [6]
RP FUNCTION, INTERACTION WITH CEH-63, AND DISRUPTION PHENOTYPE.
RX PubMed=22207033; DOI=10.1016/j.gene.2011.11.042;
RA Feng H., Reece-Hoyes J.S., Walhout A.J., Hope I.A.;
RT "A regulatory cascade of three transcription factors in a single specific
RT neuron, DVC, in Caenorhabditis elegans.";
RL Gene 494:73-84(2012).
RN [7] {ECO:0000305}
RP INTERACTION WITH LIM-7, AND TISSUE SPECIFICITY.
RX PubMed=28676648; DOI=10.1038/s41598-017-04587-8;
RA Bhati M., Llamosas E., Jacques D.A., Jeffries C.M., Dastmalchi S.,
RA Ripin N., Nicholas H.R., Matthews J.M.;
RT "Interactions between LHX3- and ISL1-family LIM-homeodomain transcription
RT factors are conserved in Caenorhabditis elegans.";
RL Sci. Rep. 7:4579-4579(2017).
CC -!- FUNCTION: Probable transcription factor, modulating expression of
CC helix-loop-helix protein mbr-1 and homeobox protein ceh-63, perhaps
CC acting in concert with ceh-63 (PubMed:22207033). Confers thermosensory
CC function to neurons (PubMed:10774727). Required for correct AFD-
CC mediated thermotaxis (PubMed:10774727). Involved in postembryonic
CC differentiation of the ALA neuron, and regulation of genes that
CC contribute to behavioral quiescence, a sleep-like behavior mediated by
CC ALA (PubMed:20501595). Regulates its own expression and also that of
CC homeodomain ceh-17, together forming an autoregulatory loop in the ALA
CC neuron (PubMed:20501595). Required for initial pathfinding of the ALA
CC axons, but largely dispensable for axon migration (PubMed:20501595).
CC Involved in regulating postembryonic axon maintenance in the ventral
CC nerve cord, acting in concert with LIM homeobox protein lim-6, via
CC modulation of expression of immunoglobulin domain zig genes in the
CC interneuron PVT (PubMed:12490565). {ECO:0000269|PubMed:10774727,
CC ECO:0000269|PubMed:12490565, ECO:0000269|PubMed:20501595,
CC ECO:0000269|PubMed:22207033}.
CC -!- SUBUNIT: Interacts (via LIM zinc-binding domains 1 and 2) with lim-7
CC (via LID domain) (PubMed:28676648). May interact with itself
CC (PubMed:22207033). May interact with homeobox protein ceh-63
CC (PubMed:22207033). {ECO:0000269|PubMed:22207033,
CC ECO:0000269|PubMed:28676648}.
CC -!- INTERACTION:
CC P20271; G5EC36: lim-7; NbExp=3; IntAct=EBI-2411837, EBI-324631;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:10774727}.
CC -!- TISSUE SPECIFICITY: Expressed in the anterior AFDL/R sensory neurons
CC and BDUL/R and ALA interneurons, and in PVT, PVQL/R, DVC, PVNL/R,
CC PVWL/R, PVR, PHCL/R, PHAL/R and PHBL/R cells in the tail region.
CC {ECO:0000269|PubMed:10774727, ECO:0000269|PubMed:20501595,
CC ECO:0000269|PubMed:28676648}.
CC -!- DEVELOPMENTAL STAGE: Late embryonic, larval and adult stages
CC (PubMed:10774727). Expressed in the interneuron PVT during
CC embryogenesis (PubMed:12490565). {ECO:0000269|PubMed:10774727,
CC ECO:0000269|PubMed:12490565}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes severe defects in
CC the ALA neuron in young adults (PubMed:20501595). RNAi-mediated
CC knockdown abolishes expression of helix-loop-helix protein mbr-1 in the
CC interneuron DVC (PubMed:22207033). {ECO:0000269|PubMed:20501595,
CC ECO:0000269|PubMed:22207033}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF244368; AAF77181.1; -; mRNA.
DR EMBL; FO081392; CCD71294.1; -; Genomic_DNA.
DR PIR; S05710; S05710.
DR RefSeq; NP_509273.1; NM_076872.3.
DR AlphaFoldDB; P20271; -.
DR SASBDB; P20271; -.
DR SMR; P20271; -.
DR BioGRID; 45936; 29.
DR IntAct; P20271; 26.
DR STRING; 6239.F46C8.5; -.
DR PaxDb; P20271; -.
DR EnsemblMetazoa; F46C8.5.1; F46C8.5.1; WBGene00000438.
DR GeneID; 181012; -.
DR KEGG; cel:CELE_F46C8.5; -.
DR UCSC; F46C8.5; c. elegans.
DR CTD; 181012; -.
DR WormBase; F46C8.5; CE28335; WBGene00000438; ceh-14.
DR eggNOG; KOG4577; Eukaryota.
DR GeneTree; ENSGT00940000167674; -.
DR HOGENOM; CLU_027802_2_1_1; -.
DR InParanoid; P20271; -.
DR OMA; CKDDYEQ; -.
DR OrthoDB; 948890at2759; -.
DR PhylomeDB; P20271; -.
DR SignaLink; P20271; -.
DR PRO; PR:P20271; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00000438; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0048666; P:neuron development; IMP:WormBase.
DR GO; GO:0030182; P:neuron differentiation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0040040; P:thermosensory behavior; IMP:WormBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox; LIM domain;
KW Metal-binding; Neurogenesis; Nucleus; Reference proteome; Repeat; Zinc.
FT CHAIN 1..351
FT /note="LIM/homeobox protein ceh-14"
FT /id="PRO_0000075745"
FT DOMAIN 46..105
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 105..169
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 180..239
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 238..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 351 AA; 40056 MW; 1DF88B3E824AC52D CRC64;
MLGHNILTLG ECDELDNHIV MCSTGLLSPQ EDFSNVNAGH PNNEEAICSL CDKKIRDRFV
SKVNGRCYHS SCLRCSTCKD ELGATCFLRE DSMYCRAHFY KKFGTKCSSC NEGIVPDHVV
RKASNHVYHV ECFQCFICKR SLETGEEFYL IADDARLVCK DDYEQARDKH CNELEGDGSN
KRPRTTISAK SLETLKQAYQ TSSKPARHVR EQLASETGLD MRVVQVWFQN RRAKEKRLKK
DAGRRWKSSN RAESDSNSPI ESINGQSPNY LYLDHPMDDG NESNYLFHSR EQTPDKYYRN
ETPSTDPPPM HMTTPSVLTT NFSTPLSLST NVYNLPPPES QLIPHMTPQY I
