HM16_CAEEL
ID HM16_CAEEL Reviewed; 187 AA.
AC P34326; P90748; Q6DTF7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Homeobox protein engrailed-like ceh-16;
GN Name=ceh-16 {ECO:0000312|WormBase:C13G5.1};
GN ORFNames=C13G5.1 {ECO:0000312|WormBase:C13G5.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=15659483; DOI=10.1242/dev.01638;
RA Cassata G., Shemer G., Morandi P., Donhauser R., Podbilewicz B.,
RA Baumeister R.;
RT "ceh-16/engrailed patterns the embryonic epidermis of Caenorhabditis
RT elegans.";
RL Development 132:739-749(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND MUTAGENESIS OF THR-95.
RX PubMed=19607822; DOI=10.1016/j.ydbio.2009.07.005;
RA Huang X., Tian E., Xu Y., Zhang H.;
RT "The C. elegans engrailed homolog ceh-16 regulates the self-renewal
RT expansion division of stem cell-like seam cells.";
RL Dev. Biol. 333:337-347(2009).
CC -!- FUNCTION: Transcriptional regulator which binds to DNA to regulate gene
CC expression and promote seam cell development and differentiation during
CC embryogenesis (PubMed:15659483, PubMed:19607822). Plays a role in
CC maintaining the boundaries between the lateral rows of seam cells and
CC the ventral and dorsal row of epidermal cells during embryonic
CC development (PubMed:15659483). Negatively regulates the expression of
CC the fusion effector protein eff-1 to prevent seam cell fusion with the
CC dorsal and ventral epidermal cells during embryonic elongation
CC (PubMed:15659483). Positively regulates seam cell self-renewal and
CC expansion during the L2 larval stage to promote seam cell development
CC (PubMed:19607822). This role does not seem to be via regulation of eff-
CC 1 expression (PubMed:19607822). Specifically, it is required for the
CC asymmetric division of the V5.p seam cell during the L2 larval stage,
CC and in turn the asymmetric nuclear distribution of pop-1 in V5.p
CC daughter cells (PubMed:19607822). {ECO:0000269|PubMed:15659483,
CC ECO:0000269|PubMed:19607822}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:15659483, ECO:0000269|PubMed:19607822}. Cytoplasm
CC {ECO:0000269|PubMed:19607822}.
CC -!- TISSUE SPECIFICITY: Expressed in seam cells.
CC {ECO:0000269|PubMed:19607822}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryonic development
CC (PubMed:15659483). In embryos, highly expressed after the first
CC cleavage until the early 3-fold stage in one bilateral row of epidermal
CC hyp5, H0-H2, V1-V6 and T-cells (the seam cells) (PubMed:15659483). In
CC later embryonic stages, expressed in anterior neurons and in the DA1
CC and DD1 motorneurons after hatching (PubMed:15659483). Expressed in
CC seam cells at all larval and adult stages (PubMed:19607822). In
CC dividing seam cells at the L2 larval stage, expressed equally in both
CC daughters and expression in the anterior daughter ceases when it fuses
CC with hyp7 (PubMed:19607822). {ECO:0000269|PubMed:15659483,
CC ECO:0000269|PubMed:19607822}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to embryos with
CC disorganized epidermal cells, which cause morphological and elongation
CC defects (PubMed:15659483). The seam cells in these embryos abnormally
CC fuse to the syncytial hypodermis during embryonic elongation and
CC ectopically express eff-1 (PubMed:15659483). RNAi-mediated knockdown
CC results in embryonic lethality and embryos exhibit seam cell
CC differentiation defects as indicated by a decrease in expression of
CC early seam cell proteins including elt-5, nhr-73 and nhr-74
CC (PubMed:15659483). {ECO:0000269|PubMed:15659483}.
CC -!- SIMILARITY: Belongs to the engrailed homeobox family. {ECO:0000305}.
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DR EMBL; AY647457; AAT67384.1; -; mRNA.
DR EMBL; BX284603; CCD63316.1; -; Genomic_DNA.
DR PIR; S44752; S44752.
DR PIR; T15488; T15488.
DR RefSeq; NP_498898.2; NM_066497.3.
DR AlphaFoldDB; P34326; -.
DR SMR; P34326; -.
DR BioGRID; 56120; 1.
DR IntAct; P34326; 1.
DR STRING; 6239.C13G5.1.2; -.
DR EPD; P34326; -.
DR PaxDb; P34326; -.
DR EnsemblMetazoa; C13G5.1.1; C13G5.1.1; WBGene00000439.
DR EnsemblMetazoa; C13G5.1.2; C13G5.1.2; WBGene00000439.
DR GeneID; 191618; -.
DR KEGG; cel:CELE_C13G5.1; -.
DR UCSC; C13G5.1; c. elegans.
DR CTD; 191618; -.
DR WormBase; C13G5.1; CE37477; WBGene00000439; ceh-16.
DR eggNOG; KOG0493; Eukaryota.
DR GeneTree; ENSGT00940000167868; -.
DR HOGENOM; CLU_051739_3_1_1; -.
DR InParanoid; P34326; -.
DR OMA; QFQMMAQ; -.
DR OrthoDB; 1575614at2759; -.
DR PhylomeDB; P34326; -.
DR SignaLink; P34326; -.
DR PRO; PR:P34326; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00000439; Expressed in embryo and 3 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0010172; P:embryonic body morphogenesis; IMP:WormBase.
DR GO; GO:0009957; P:epidermal cell fate specification; IMP:WormBase.
DR GO; GO:0048859; P:formation of anatomical boundary; IMP:WormBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..187
FT /note="Homeobox protein engrailed-like ceh-16"
FT /id="PRO_0000196091"
FT DNA_BIND 87..146
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 14..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 60..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 144..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..100
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 95
FT /note="T->N: In bp323; reduces DNA-binding. Impairs the
FT self-renewal and expansion of seam cells at the L2 larval
FT stage, which reduces the number of seam cells in adults.
FT Defective division of the V5.p seam cell at the L2 larval
FT stage resulting in daughter cells with an equal
FT distribution of pop-1 and 65% of animals not having the PDE
FT dopaminergic neuron."
FT /evidence="ECO:0000269|PubMed:19607822"
SQ SEQUENCE 187 AA; 21008 MW; EA7753276A387DF9 CRC64;
MILKFGIERI LSSPYPCPSP TISTPATSPS SISPTFASPN GTPNIASSMY PAWVFSTRYS
DRPSAGPRHR KSRKRESTGS SGSSEEEKRP RTAFTGDQLD RLKTEFRESR YLTEKRRQEL
AHELGLNESQ IKIWFQNKRA KLKKSTSSVP RDRCSSVTPN PHNHPSIHGG YQLMAQLAKV
QARAYMP
