HM18_CAEEL
ID HM18_CAEEL Reviewed; 542 AA.
AC P41934;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Homeobox protein ceh-18;
GN Name=ceh-18 {ECO:0000303|PubMed:7958868, ECO:0000312|WormBase:ZC64.3a};
GN ORFNames=ZC64.3 {ECO:0000312|WormBase:ZC64.3a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=7958868; DOI=10.1101/gad.8.16.1935;
RA Greenstein D., Hird S., Plasterk R.H.A., Andachi Y., Kohara Y., Wang B.,
RA Finney M., Ruvkun G.;
RT "Targeted mutations in the Caenorhabditis elegans POU homeo box gene ceh-18
RT cause defects in oocyte cell cycle arrest, gonad migration, and epidermal
RT differentiation.";
RL Genes Dev. 8:1935-1948(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9405097; DOI=10.1006/dbio.1997.8728;
RA Rose K.L., Winfrey V.P., Hoffman L.H., Hall D.H., Furuta T., Greenstein D.;
RT "The POU gene ceh-18 promotes gonadal sheath cell differentiation and
RT function required for meiotic maturation and ovulation in Caenorhabditis
RT elegans.";
RL Dev. Biol. 192:59-77(1997).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12533508; DOI=10.1101/gad.1028303;
RA Miller M.A., Ruest P.J., Kosinski M., Hanks S.K., Greenstein D.;
RT "An Eph receptor sperm-sensing control mechanism for oocyte meiotic
RT maturation in Caenorhabditis elegans.";
RL Genes Dev. 17:187-200(2003).
RN [5]
RP FUNCTION, INTERACTION WITH AKIR-1, AND DISRUPTION PHENOTYPE.
RX PubMed=30036395; DOI=10.1371/journal.pgen.1007494;
RA Polanowska J., Chen J.X., Soule J., Omi S., Belougne J., Taffoni C.,
RA Pujol N., Selbach M., Zugasti O., Ewbank J.J.;
RT "Evolutionary plasticity in the innate immune function of Akirin.";
RL PLoS Genet. 14:E1007494-E1007494(2018).
CC -!- FUNCTION: Directs gonadal sheath cell differentiation and function
CC (PubMed:9405097). Also directs gonad migration and plays a role in
CC specifying the differentiated phenotypes of epidermal cells during
CC postembryonic development (PubMed:7958868). Plays a role in oogenesis,
CC regulating a sheath cell signal that causes oocytes to maintain
CC diakinesis arrest during meiosis (PubMed:7958868). Negatively regulates
CC oocyte maturation, ovulation and MAPK activation in oocytes when sperm
CC are not available for fertilization (PubMed:12533508). May be recruited
CC by akir-1 to the promoter regions of antimicrobial peptide genes to
CC control gene expression in response to fungal infection
CC (PubMed:30036395). {ECO:0000269|PubMed:12533508,
CC ECO:0000269|PubMed:30036395, ECO:0000269|PubMed:7958868,
CC ECO:0000269|PubMed:9405097}.
CC -!- SUBUNIT: Interacts with akir-1. {ECO:0000269|PubMed:30036395}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:7958868}.
CC -!- TISSUE SPECIFICITY: Expressed in the gonadal sheath cells that signal
CC the oocyte, but not in the oocyte. {ECO:0000269|PubMed:7958868}.
CC -!- DEVELOPMENTAL STAGE: First expressed in embryos at the three-fold stage
CC (PubMed:7958868). After hatching expressed in the syncytial hypodermal
CC cells located throughout the body, pharyngeal muscle, seam cells P
CC hypodermal blast cells, body muscle and anterior touch cells
CC (PubMed:7958868). Expressed in the distal tip cell of the somatic ginad
CC from the L2 stage of larval development through to the adult stage
CC (PubMed:7958868). {ECO:0000269|PubMed:7958868}.
CC -!- DISRUPTION PHENOTYPE: Viable, but some animals exhibit sterile and
CC lethal phenotypes including hermaphrodite sterility, embryonic
CC lethality and larval arrest (PubMed:7958868). Defective ovulation and
CC terminal differentiation of oocytes, which result from a delay or an
CC inability to complete meiosis during oogenesis (PubMed:7958868,
CC PubMed:9405097). Defects in gonadal migration (PubMed:7958868).
CC Defective sheath cell differentiation in terms of cell shape and
CC position and defective functions which include weaker sheath cell
CC contractions (PubMed:9405097). Abnormal hypodermal cell differentiation
CC (PubMed:7958868). Defective MAPK activation in oocytes in the presence
CC and absence of sperm (PubMed:12533508). RNAi-mediated knockdown reduces
CC survival following fungal infection by D.coniospora (PubMed:30036395).
CC {ECO:0000269|PubMed:12533508, ECO:0000269|PubMed:30036395,
CC ECO:0000269|PubMed:7958868, ECO:0000269|PubMed:9405097}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family.
CC {ECO:0000305}.
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DR EMBL; U16367; AAA52203.1; -; mRNA.
DR EMBL; BX284606; CCD71431.1; -; Genomic_DNA.
DR PIR; A54963; A54963.
DR RefSeq; NP_741758.1; NM_171659.4.
DR AlphaFoldDB; P41934; -.
DR SMR; P41934; -.
DR BioGRID; 45607; 23.
DR IntAct; P41934; 15.
DR STRING; 6239.ZC64.3a; -.
DR EPD; P41934; -.
DR PaxDb; P41934; -.
DR EnsemblMetazoa; ZC64.3.1; ZC64.3.1; WBGene00000441.
DR GeneID; 180671; -.
DR KEGG; cel:CELE_ZC64.3; -.
DR UCSC; ZC64.3b; c. elegans.
DR CTD; 180671; -.
DR WormBase; ZC64.3a; CE25674; WBGene00000441; ceh-18.
DR eggNOG; KOG3802; Eukaryota.
DR GeneTree; ENSGT00940000171806; -.
DR HOGENOM; CLU_039438_0_0_1; -.
DR InParanoid; P41934; -.
DR OMA; DMNQRNA; -.
DR OrthoDB; 834648at2759; -.
DR PhylomeDB; P41934; -.
DR Reactome; R-CEL-6807505; RNA polymerase II transcribes snRNA genes.
DR SignaLink; P41934; -.
DR PRO; PR:P41934; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00000441; Expressed in larva and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008544; P:epidermis development; IMP:WormBase.
DR GO; GO:0008354; P:germ cell migration; IMP:WormBase.
DR GO; GO:1900194; P:negative regulation of oocyte maturation; IMP:UniProtKB.
DR GO; GO:0001556; P:oocyte maturation; IMP:WormBase.
DR GO; GO:0030728; P:ovulation; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..542
FT /note="Homeobox protein ceh-18"
FT /id="PRO_0000100782"
FT DOMAIN 290..364
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 421..480
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 243..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 514..542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 542 AA; 59480 MW; 34A97C7CC4B850E6 CRC64;
MNTLENTFQF LAEVFEANGG CLVTERVSPS LSGASKRKSR PVKRIITEDE VIPEEEVVEE
EEEEKVEQPS EEVTCPAMQV DATDAILASL RQIANNDLSE HCIREDEGNT SAEVFSNGNG
EDMEQAGLAG TDLLRRVADM LQGNHNGSLN SHFLQQQFAA FKETVDPTPV STNGNGLLTP
ITTQLSPLFT STDAFNSPEK LLQAIMTPSL GFLGANGLGA SNSGLLSSPL IAPSPTLLQS
LMNTPTQPTA SLTPKKAENR PPVVSQTLKA SKRRLFDDTS RIEAASMSGD DRIDMNELEA
FAQTFKKQRI KFGFTQGDVG VALGKRYGTD FSQTTISRFE ALNLSFKNMC KLRPLLKEWL
ADVEMAIEGG ATVTDLIDKK TIHNGNHHTI HHVDIHETSI SNSISSVTAS SLLSREQHVK
RRRKRTNLDM NQRNALDTFF ALNPRPDHDK MTDIANSLEL DRDVVRVWFC NRRQKMRRVD
EPIEGEMVTP SVSPVFPHFS SMSALEQIQE AARLASCQAS NDDSDGTSGS PDAPSNDGCS
DL
