HM20_CAEEL
ID HM20_CAEEL Reviewed; 338 AA.
AC P41779;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Homeobox protein ceh-20;
GN Name=ceh-20 {ECO:0000312|WormBase:F31E3.1};
GN ORFNames=F31E3.1 {ECO:0000312|WormBase:F31E3.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2;
RA Buerglin T.R., Lai E., Ruvkun G.;
RL Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP DISCUSSION OF SEQUENCE.
RX PubMed=1363814; DOI=10.1038/ng0892-319;
RA Burglin T.R., Ruvkun G.;
RT "New motif in PBX genes.";
RL Nat. Genet. 1:319-320(1992).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF ARG-245.
RX PubMed=15750187; DOI=10.1242/dev.01569;
RA Yang L., Sym M., Kenyon C.;
RT "The roles of two C. elegans HOX co-factor orthologs in cell migration and
RT vulva development.";
RL Development 132:1413-1428(2005).
RN [5]
RP FUNCTION, INTERACTION WITH PSA-3 AND NOB-1, AND SUBCELLULAR LOCATION.
RX PubMed=16824957; DOI=10.1016/j.devcel.2006.04.020;
RA Arata Y., Kouike H., Zhang Y., Herman M.A., Okano H., Sawa H.;
RT "Wnt signaling and a Hox protein cooperatively regulate psa-3/Meis to
RT determine daughter cell fate after asymmetric cell division in C.
RT elegans.";
RL Dev. Cell 11:105-115(2006).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18316179; DOI=10.1016/j.mod.2008.01.009;
RA Jiang Y., Shi H., Amin N.M., Sultan I., Liu J.;
RT "Mesodermal expression of the C. elegans HMX homolog mls-2 requires the PBC
RT homolog CEH-20.";
RL Mech. Dev. 125:451-461(2008).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21307099; DOI=10.1242/dev.062240;
RA Tian C., Shi H., Colledge C., Stern M., Waterston R., Liu J.;
RT "The C. elegans SoxC protein SEM-2 opposes differentiation factors to
RT promote a proliferative blast cell fate in the postembryonic mesoderm.";
RL Development 138:1033-1043(2011).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP 102-GLN--SER-338; PRO-214 AND ARG-245.
RX PubMed=23788625; DOI=10.1101/gad.217224.113;
RA Doitsidou M., Flames N., Topalidou I., Abe N., Felton T., Remesal L.,
RA Popovitchenko T., Mann R., Chalfie M., Hobert O.;
RT "A combinatorial regulatory signature controls terminal differentiation of
RT the dopaminergic nervous system in C. elegans.";
RL Genes Dev. 27:1391-1405(2013).
CC -!- FUNCTION: Transcription factor that binds to the 5'-TGATNNAT(G/T)(G/A)-
CC 3' PBC/Hox lineage enhancer region of sem-2 to promote cell fate
CC specification in the postembryonic mesoderm (also known as the M
CC lineage) (PubMed:21307099). Required for the M lineage-specific
CC expression of the transcription factor, mls-2 (PubMed:18316179).
CC Required for asymmetric division of the T hypodermal cell, probably
CC acting via the regulation of asymmetric expression of Meis protein psa-
CC 3 in concert with homeobox protein nob-1 and the Wnt-MAPK pathway
CC (PubMed:16824957). Has a role in the mig-13 pathway to promote the
CC guidance, migration and positioning of Q neuroblasts and their
CC descendants along the anteroposterior body axis and the anterior
CC migration of BDU interneurons (PubMed:15750187). Also required for
CC normal vulval formation (PubMed:15750187). Plays a role in regulating
CC gene expression in dopaminergic neurons, acting in midbody PDE neurons,
CC and acting redundantly with ceh-40 in head neurons (PubMed:23788625).
CC May activate dopamine pathway genes in concert with ETS domain-
CC containing protein ast-1, and homeobox proteins ceh-43 and ceh-40
CC (PubMed:23788625). {ECO:0000269|PubMed:15750187,
CC ECO:0000269|PubMed:16824957, ECO:0000269|PubMed:18316179,
CC ECO:0000269|PubMed:21307099, ECO:0000269|PubMed:23788625}.
CC -!- SUBUNIT: Interacts with Meis protein psa-3 (PubMed:16824957). Interacts
CC with homeobox protein nob-1 (PubMed:16824957).
CC {ECO:0000269|PubMed:16824957}.
CC -!- INTERACTION:
CC P41779; Q9N5D6: unc-62; NbExp=3; IntAct=EBI-2416925, EBI-319775;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15750187,
CC ECO:0000269|PubMed:16824957, ECO:0000305|PubMed:21307099}.
CC Note=Localized to the nucleus, in the T hypodermal cell, in a psa-3-
CC dependent manner. {ECO:0000269|PubMed:16824957}.
CC -!- TISSUE SPECIFICITY: Expressed in head dopaminergic neurons.
CC {ECO:0000269|PubMed:23788625}.
CC -!- DEVELOPMENTAL STAGE: Expressed in QR and QL neuroblasts, P and V cells
CC and all their respective descendents, M cells, BDU interneurons, ALM
CC and HSM neurons, body wall muscles, I4, all ventral cord neurons and in
CC a few unidentified neurons in the head behind the posterior bulb of the
CC pharynx during the L1 stage of larval development (PubMed:15750187).
CC Expressed in V cell descendents from the larval stage of development to
CC adulthood (PubMed:15750187). Expressed in the midbody dopaminergic PDE
CC neurons throughout life (PubMed:23788625).
CC {ECO:0000269|PubMed:15750187, ECO:0000269|PubMed:23788625}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in egg laying
CC defects, abnormal migration of QR neuroblast lineage cells and vulval
CC protrusions due to ectopic induction and defective morphogenesis of
CC vulval precursors cells (PubMed:15750187). RNAi-mediated knockdown
CC results in the absence of the transcription factor, mls-2, in the M
CC lineage (PubMed:18316179). {ECO:0000269|PubMed:15750187,
CC ECO:0000269|PubMed:18316179}.
CC -!- SIMILARITY: Belongs to the TALE/PBX homeobox family. {ECO:0000305}.
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DR EMBL; U01303; AAC46471.1; -; mRNA.
DR EMBL; BX284603; CCD63401.1; -; Genomic_DNA.
DR PIR; T16221; T16221.
DR RefSeq; NP_001022555.1; NM_001027384.3.
DR AlphaFoldDB; P41779; -.
DR SMR; P41779; -.
DR BioGRID; 532863; 2.
DR IntAct; P41779; 2.
DR STRING; 6239.F31E3.1; -.
DR EPD; P41779; -.
DR PaxDb; P41779; -.
DR PeptideAtlas; P41779; -.
DR EnsemblMetazoa; F31E3.1.1; F31E3.1.1; WBGene00000443.
DR GeneID; 3565545; -.
DR KEGG; cel:CELE_F31E3.1; -.
DR UCSC; F31E3.1; c. elegans.
DR CTD; 3565545; -.
DR WormBase; F31E3.1; CE01241; WBGene00000443; ceh-20.
DR eggNOG; KOG0774; Eukaryota.
DR GeneTree; ENSGT00940000169272; -.
DR HOGENOM; CLU_041153_0_0_1; -.
DR InParanoid; P41779; -.
DR OMA; HALHEPP; -.
DR OrthoDB; 804740at2759; -.
DR PhylomeDB; P41779; -.
DR PRO; PR:P41779; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00000443; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IDA:WormBase.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; TAS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001223; F:transcription coactivator binding; IPI:WormBase.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; TAS:WormBase.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:UniProtKB.
DR GO; GO:0048568; P:embryonic organ development; IBA:GO_Central.
DR GO; GO:0007498; P:mesoderm development; IMP:WormBase.
DR GO; GO:0007501; P:mesodermal cell fate specification; IGI:WormBase.
DR GO; GO:0042692; P:muscle cell differentiation; IGI:WormBase.
DR GO; GO:0048666; P:neuron development; IBA:GO_Central.
DR GO; GO:0048337; P:positive regulation of mesodermal cell fate specification; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0009786; P:regulation of asymmetric cell division; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR005542; PBX.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03792; PBC; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS51978; PBC; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..338
FT /note="Homeobox protein ceh-20"
FT /id="PRO_0000048991"
FT DOMAIN 4..187
FT /note="PBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT DNA_BIND 188..250
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 11..91
FT /note="PBC-A"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT REGION 94..187
FT /note="PBC-B"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01322"
FT MUTAGEN 102..338
FT /note="Missing: In ay38; loss of dopamine transporter dat-1
FT expression in midbody neurons (PDE)."
FT /evidence="ECO:0000269|PubMed:23788625"
FT MUTAGEN 214
FT /note="P->L: In ay42; loss of dopamine transporter dat-1
FT expression in midbody neurons (PDE)."
FT /evidence="ECO:0000269|PubMed:23788625"
FT MUTAGEN 245
FT /note="R->H: In mu290; egg laying defects, abnormal
FT migration of QR neuroblast lineage cells and BDU
FT interneurons, and vulval protrusions. Loss of expression of
FT dopamine pathway genes, including dopamine transporter dat-
FT 1, in midbody neurons (PDE)."
FT /evidence="ECO:0000269|PubMed:15750187,
FT ECO:0000269|PubMed:23788625"
SQ SEQUENCE 338 AA; 38154 MW; 5B2581B39519B4DB CRC64;
MVGTHPANLS ELLDAVLKIN EQTLDDNDSA KKQELQCHPM RQALFDVLCE TKEKTVLTVR
NQVDETPEDP QLMRLDNMLV AEGVAGPDKG GSLGSDASGG DQADYRQKLH QIRVLYNEEL
RKYEEACNEF TQHVRSLLKD QSQVRPIAHK EIERMVYIIQ RKFNGIQVQL KQSTCEAVMI
LRSRFLDARR KRRNFSKQAT EVLNEYFYGH LSNPYPSEEA KEDLARQCNI TVSQVSNWFG
NKRIRYKKNM AKAQEEASMY AAKKNAHVTL GGMAGNPYGM LPGAAAAAGL LNPYNPMNIP
GQDTLHMGMP PFDLSVYNPQ LMAAAQYQQQ MDNADKNS
