HM32_CAEEL
ID HM32_CAEEL Reviewed; 439 AA.
AC Q23175; Q9BMH2;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Homeobox protein ceh-32;
GN Name=ceh-32 {ECO:0000312|WormBase:W05E10.3};
GN ORFNames=W05E10.3 {ECO:0000312|WormBase:W05E10.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo;
RX PubMed=11476572; DOI=10.1006/dbio.2001.0325;
RA Dozier C., Kagoshima H., Niklaus G., Cassata G., Buerglin T.R.;
RT "The Caenorhabditis elegans Six/sine oculis class homeobox gene ceh-32 is
RT required for head morphogenesis.";
RL Dev. Biol. 236:289-303(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP INTERACTION WITH GMN-1.
RX PubMed=15811859; DOI=10.1074/jbc.c500070200;
RA Yanagi K., Mizuno T., Tsuyama T., Tada S., Iida Y., Sugimoto A., Eki T.,
RA Enomoto T., Hanaoka F.;
RT "Caenorhabditis elegans geminin homologue participates in cell cycle
RT regulation and germ line development.";
RL J. Biol. Chem. 280:19689-19694(2005).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=30890567; DOI=10.1242/dev.168153;
RA Brandt J.P., Rossillo M., Du Z., Ichikawa D., Barnes K., Chen A., Noyes M.,
RA Bao Z., Ringstad N.;
RT "Lineage context switches the function of a C. elegans Pax6 homolog in
RT determining a neuronal fate.";
RL Development 146:0-0(2019).
CC -!- FUNCTION: Transcription factor which binds a motif with the core
CC sequence 5'-GTATCA-3' (PubMed:30890567). Plays a role in head
CC morphogenesis (PubMed:11476572). May cooperate with the transcription
CC factor vab-3 and phosphatase eya-1 to repress transcription factor ets-
CC 5 expression in non BAG neuronal cells (PubMed:30890567).
CC {ECO:0000269|PubMed:11476572, ECO:0000269|PubMed:30890567}.
CC -!- SUBUNIT: Interacts with gmn-1. {ECO:0000269|PubMed:15811859}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11476572}.
CC -!- TISSUE SPECIFICITY: Expressed in the posterior gonad (PubMed:11476572).
CC Expressed in some cells in the head that are probably neurons
CC (PubMed:30890567). Not expressed in BAG neurons (PubMed:30890567).
CC {ECO:0000269|PubMed:11476572, ECO:0000269|PubMed:30890567}.
CC -!- DEVELOPMENTAL STAGE: Expression starts during embryonic gastrulation
CC and persists through to adults (PubMed:11476572). Expressed in neuronal
CC precursor cells during embryogenesis (PubMed:11476572). Upon hatching,
CC expression is also seen in sheath/spermatheca precursor cells and later
CC in gonadal sheath cells (PubMed:11476572). Expressed in head hypodermal
CC cells (hyp3, hyp4, hyp5 and hyp6) in larvae (PubMed:11476572).
CC {ECO:0000269|PubMed:11476572}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in hermaphrodite adults
CC causes embryonic and larval lethality and head morphogenesis defects in
CC L1 and L2 larval stages of offspring. {ECO:0000269|PubMed:11476572}.
CC -!- SIMILARITY: Belongs to the SIX/Sine oculis homeobox family.
CC {ECO:0000305}.
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DR EMBL; AF325178; AAG49583.1; -; mRNA.
DR EMBL; BX284605; CAB01249.2; -; Genomic_DNA.
DR PIR; T26192; T26192.
DR RefSeq; NP_505958.1; NM_073557.4.
DR AlphaFoldDB; Q23175; -.
DR SMR; Q23175; -.
DR BioGRID; 44628; 7.
DR IntAct; Q23175; 1.
DR STRING; 6239.W05E10.3; -.
DR EPD; Q23175; -.
DR PaxDb; Q23175; -.
DR PRIDE; Q23175; -.
DR EnsemblMetazoa; W05E10.3.1; W05E10.3.1; WBGene00000453.
DR GeneID; 179603; -.
DR KEGG; cel:CELE_W05E10.3; -.
DR UCSC; W05E10.3.1; c. elegans.
DR CTD; 179603; -.
DR WormBase; W05E10.3; CE29364; WBGene00000453; ceh-32.
DR eggNOG; KOG0775; Eukaryota.
DR GeneTree; ENSGT00940000169263; -.
DR HOGENOM; CLU_049495_0_0_1; -.
DR InParanoid; Q23175; -.
DR OMA; EMWHEAH; -.
DR OrthoDB; 1061244at2759; -.
DR SignaLink; Q23175; -.
DR PRO; PR:Q23175; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00000453; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009886; P:post-embryonic animal morphogenesis; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:WormBase.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR031701; SIX1_SD.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF16878; SIX1_SD; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..439
FT /note="Homeobox protein ceh-32"
FT /id="PRO_0000048998"
FT DNA_BIND 183..243
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 253..293
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 379..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 379..394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..428
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 439 AA; 49637 MW; D4001E3A5F350C37 CRC64;
MFTPEQFTKV MSQLGNFSQL GQMFQPGNVA MLQALQANGA SSTPSLFPAM PSVIPSLAAP
SSPTTSNLTA DQIVKTCEQL ETDGDVDGLF RFMCTIPPQK TQEVAGNEAF LRARALVCFH
ASHFRELYAI LENNKFSPKY HPKLQEMWHE AHYREQEKNR GKSLCAVDKY RVRKKYPMPR
TIWDGEQKTH CFKERTRSLL REWYLKDPYP NPPKKKELAN ATGLTQMQVG NWFKNRRQRD
RAAAAKNKQN IIGVELKKTS SDMSDSDDDF EDSMTDSPSP IDEPKDLSKS HIPKLSPTLL
PKMATPFDMF AAAANPLMML NLNPALYMQF HNFFNTMRNP QIDEEENSET TVEVEADIEP
PKKRSKLSID EILNIKSEVS PSQCSPCSNE SLSPKRAVKT EEVKKEDDEA AEEDSRSVKS
ETSEDPKHSS PKSTTSQSE
