HM34_CAEEL
ID HM34_CAEEL Reviewed; 256 AA.
AC Q94165;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Homeobox protein ceh-34;
GN Name=ceh-34; ORFNames=C10G8.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, INTERACTION WITH EYA-1, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=19427847; DOI=10.1016/j.ydbio.2009.05.538;
RA Amin N.M., Lim S.E., Shi H., Chan T.L., Liu J.;
RT "A conserved Six-Eya cassette acts downstream of Wnt signaling to direct
RT non-myogenic versus myogenic fates in the C. elegans postembryonic
RT mesoderm.";
RL Dev. Biol. 331:350-360(2009).
RN [3]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=20335356; DOI=10.1242/dev.048496;
RA Amin N.M., Shi H., Liu J.;
RT "The FoxF/FoxC factor LET-381 directly regulates both cell fate
RT specification and cell differentiation in C. elegans mesoderm
RT development.";
RL Development 137:1451-1460(2010).
RN [4]
RP FUNCTION, INTERACTION WITH EYA-1, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=20713707; DOI=10.1073/pnas.1010023107;
RA Hirose T., Galvin B.D., Horvitz H.R.;
RT "Six and Eya promote apoptosis through direct transcriptional activation of
RT the proapoptotic BH3-only gene egl-1 in Caenorhabditis elegans.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:15479-15484(2010).
CC -!- FUNCTION: Acts as a transcription regulator (PubMed:20713707). Binds to
CC the sequence motif 5'-TCAGGTT-3' (PubMed:20335356). Binds to the cis-
CC regulatory element of proapoptotic factor egl-1 gene and together with
CC eya-1 activates egl-1 expression to promote motor neuron M4 sister cell
CC apoptosis (PubMed:20713707). Also promotes apoptosis of I1 pharyngeal
CC neuron sister cell (PubMed:20713707). Together with eya-1, required to
CC specify the coelomocyte fate in embryonic and postembryonic precursors
CC (PubMed:19427847, PubMed:20335356). {ECO:0000269|PubMed:19427847,
CC ECO:0000269|PubMed:20335356, ECO:0000269|PubMed:20713707}.
CC -!- SUBUNIT: Interacts (via N-terminus) with eya-1 (via C-terminus).
CC {ECO:0000269|PubMed:19427847, ECO:0000269|PubMed:20713707}.
CC -!- INTERACTION:
CC Q94165; O17670: eya-1; NbExp=3; IntAct=EBI-2413136, EBI-311862;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19427847,
CC ECO:0000269|PubMed:20713707}.
CC -!- DEVELOPMENTAL STAGE: Expression starts at the embryonic bean stage and
CC continues throughout larval and adult stages in few anterior body wall
CC muscle cells and in unidentified cells in the head (PubMed:19427847).
CC Expressed predominantly in pharyngeal cells during embryogenesis and
CC throughout larval and adult stages (PubMed:20713707). In L1 larvae,
CC expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2,
CC I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2)
CC and pharyngeal epithelial cells (e1 and e3), and some body wall muscles
CC around the anterior pharynx (PubMed:20713707). Expressed transiently in
CC the M lineage-derived coelomocyte precursor cells M.dlpa and M.drpa
CC before their differentiation into coelomocytes (PubMed:19427847,
CC PubMed:20335356). Expressed in the M4 motor neuron sister cell
CC (PubMed:20713707). {ECO:0000269|PubMed:19427847,
CC ECO:0000269|PubMed:20335356, ECO:0000269|PubMed:20713707}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in embryos causes lethal
CC arrest at the embryonic 3-fold stage or at the L1 larval stage with
CC abnormal anterior morphology (PubMed:19427847). The few surviving
CC animals lack 1 or both pairs of embryonic MS lineage-derived
CC coelomocytes as well as the 2 post embryonic M lineage-derived
CC coelomocytes (PubMed:19427847). RNAi-mediated knockdown in L1 larvae
CC causes the loss of the M lineage-derived coelomocytes due to the
CC abnormal differentiation of coelomocyte precursors M.drpa and M.dlpa
CC into body wall muscle cells (PubMed:19427847). In 38 percent of
CC animals, the motor neuron M4 sister survived (PubMed:20713707).
CC {ECO:0000269|PubMed:19427847, ECO:0000269|PubMed:20713707}.
CC -!- SIMILARITY: Belongs to the SIX/Sine oculis homeobox family.
CC {ECO:0000305}.
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DR EMBL; FO080497; CCD64164.1; -; Genomic_DNA.
DR PIR; F89046; F89046.
DR RefSeq; NP_504419.1; NM_072018.6.
DR AlphaFoldDB; Q94165; -.
DR SMR; Q94165; -.
DR BioGRID; 43968; 2.
DR IntAct; Q94165; 2.
DR STRING; 6239.C10G8.6; -.
DR PaxDb; Q94165; -.
DR EnsemblMetazoa; C10G8.6.1; C10G8.6.1; WBGene00000455.
DR GeneID; 178919; -.
DR KEGG; cel:CELE_C10G8.6; -.
DR UCSC; C10G8.6; c. elegans.
DR CTD; 178919; -.
DR WormBase; C10G8.6; CE20496; WBGene00000455; ceh-34.
DR eggNOG; KOG0775; Eukaryota.
DR GeneTree; ENSGT00940000167410; -.
DR HOGENOM; CLU_046914_4_0_1; -.
DR InParanoid; Q94165; -.
DR OMA; SVHHDTI; -.
DR OrthoDB; 1453460at2759; -.
DR PhylomeDB; Q94165; -.
DR PRO; PR:Q94165; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00000455; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0007501; P:mesodermal cell fate specification; IMP:WormBase.
DR GO; GO:1904747; P:positive regulation of apoptotic process involved in development; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR031701; SIX1_SD.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF16878; SIX1_SD; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..256
FT /note="Homeobox protein ceh-34"
FT /id="PRO_0000049000"
FT DNA_BIND 134..193
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 187..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..233
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 256 AA; 29622 MW; 4283D31FD0A04D7C CRC64;
MQQSYNPQNS LTATTSYSEQ EIVCICESLF NEGLQTGRTE QLANFIYNLP QCYQVMESVL
KAQALVYFTT QNWKMLYKLL ECSKFSPHNH TVLQNLWLDA HYKEAAKTKD RELGAVCKYR
IRKKNPFPNT IWDGEETNYC FKSKSRNVLR DAYKKCQYPS VEDKRRLAQQ TELSIIQVSN
WFKNKRQRER AAGQLDRSSA RSNDSDDGSS GCESKPPMNI DSPAPPPLPT SFDLQPYYPS
PYTFAPHCDF SYIQNL
