HM35_CAEEL
ID HM35_CAEEL Reviewed; 335 AA.
AC O17894;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Homeobox protein unc-39 {ECO:0000305};
DE AltName: Full=Homeobox protein ceh-35 {ECO:0000312|WormBase:F56A12.1};
DE AltName: Full=Uncoordinated protein 39 {ECO:0000312|WormBase:F56A12.1};
GN Name=unc-39 {ECO:0000312|WormBase:F56A12.1};
GN Synonyms=ceh-35 {ECO:0000312|WormBase:F56A12.1},
GN mig-3 {ECO:0000312|WormBase:F56A12.1};
GN ORFNames=F56A12.1 {ECO:0000312|WormBase:F56A12.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF ILE-220.
RX PubMed=2361334; DOI=10.1002/dvg.1020110107;
RA Manser J., Wood W.B.;
RT "Mutations affecting embryonic cell migrations in Caenorhabditis elegans.";
RL Dev. Genet. 11:49-64(1990).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF ARG-203; ILE-220 AND PRO-283.
RX PubMed=15282156; DOI=10.1016/j.ydbio.2004.05.010;
RA Yanowitz J.L., Shakir M.A., Hedgecock E., Hutter H., Fire A.Z.,
RA Lundquist E.A.;
RT "UNC-39, the C. elegans homolog of the human myotonic dystrophy-associated
RT homeodomain protein Six5, regulates cell motility and differentiation.";
RL Dev. Biol. 272:389-402(2004).
RN [4] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF ARG-203 AND PRO-298.
RX PubMed=27855782; DOI=10.7554/elife.19887;
RA Lim M.A., Chitturi J., Laskova V., Meng J., Findeis D., Wiekenberg A.,
RA Mulcahy B., Luo L., Li Y., Lu Y., Hung W., Qu Y., Ho C.Y., Holmyard D.,
RA Ji N., McWhirter R., Samuel A.D., Miller D.M., Schnabel R., Calarco J.A.,
RA Zhen M.;
RT "Neuroendocrine modulation sustains the C. elegans forward motor state.";
RL Elife 5:0-0(2016).
RN [5]
RP ERRATUM OF PUBMED:27855782.
RX PubMed=28271995; DOI=10.7554/elife.26528;
RA Lim M., Chitturi J., Laskova V., Meng J., Findeis D., Wiekenberg A.,
RA Mulcahy B., Luo L., Li Y., Lu Y., Hung W., Qu Y., Ho C., Holmyard D.,
RA Ji N., McWhirter R.D., Samuel A.D., Miller D.M., Schnabel R., Calarco J.A.,
RA Zhen M.;
RT "Correction: Neuroendocrine modulation sustains the C. elegans forward
RT motor state.";
RL Elife 6:0-0(2017).
CC -!- FUNCTION: Probable transcription factor required for differentiation
CC and migration of neuronal cells, such as RID and CAN neurons
CC (PubMed:2361334, PubMed:15282156). Specifically, plays a role in the
CC terminal differentiation of RID peptidergic neurons (PubMed:27855782).
CC Also required for CAN neuron axon guidance (PubMed:15282156).
CC {ECO:0000269|PubMed:15282156, ECO:0000269|PubMed:2361334,
CC ECO:0000269|PubMed:27855782}.
CC -!- INTERACTION:
CC O17894; O17670: eya-1; NbExp=5; IntAct=EBI-317698, EBI-311862;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:15282156}.
CC -!- DEVELOPMENTAL STAGE: Expressed in muscles and neurons from
CC embryogenesis to adulthood (PubMed:15282156, PubMed:27855782). First
CC expressed post-fertilization, then accumulates in anterior cells of the
CC embryo that give rise to neuronal and mesodermal tissues and body wall
CC muscle cells, where expression persists and increases
CC (PubMed:15282156). Also expressed in posterior nuclei post-
CC fertilization (PubMed:15282156). At the 2-fold stage of embryogenesis,
CC expression is restricted to fewer cells (PubMed:15282156).
CC {ECO:0000269|PubMed:15282156, ECO:0000269|PubMed:27855782}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in 60% larval
CC lethality (PubMed:15282156). Surviving animals display uncoordinated
CC locomotion, withered tail morphology, and defects in both CAN neuronal
CC cell migration and axon guidance (PubMed:15282156).
CC {ECO:0000269|PubMed:15282156}.
CC -!- SIMILARITY: Belongs to the SIX/Sine oculis homeobox family.
CC {ECO:0000305}.
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DR EMBL; BX284605; CAB04483.2; -; Genomic_DNA.
DR PIR; T22761; T22761.
DR RefSeq; NP_506563.2; NM_074162.2.
DR AlphaFoldDB; O17894; -.
DR SMR; O17894; -.
DR DIP; DIP-24571N; -.
DR IntAct; O17894; 8.
DR STRING; 6239.F56A12.1; -.
DR PaxDb; O17894; -.
DR EnsemblMetazoa; F56A12.1.1; F56A12.1.1; WBGene00006775.
DR GeneID; 191623; -.
DR KEGG; cel:CELE_F56A12.1; -.
DR UCSC; F56A12.1; c. elegans.
DR CTD; 191623; -.
DR WormBase; F56A12.1; CE37921; WBGene00006775; unc-39.
DR eggNOG; KOG0775; Eukaryota.
DR GeneTree; ENSGT00940000167410; -.
DR HOGENOM; CLU_872206_0_0_1; -.
DR InParanoid; O17894; -.
DR OMA; RYKESQL; -.
DR OrthoDB; 1360664at2759; -.
DR PhylomeDB; O17894; -.
DR PRO; PR:O17894; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00006775; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0040011; P:locomotion; IMP:WormBase.
DR GO; GO:0008078; P:mesodermal cell migration; IMP:WormBase.
DR GO; GO:0001764; P:neuron migration; IMP:UniProtKB.
DR GO; GO:0018991; P:oviposition; IMP:WormBase.
DR GO; GO:0040017; P:positive regulation of locomotion; IMP:UniProtKB.
DR GO; GO:0030334; P:regulation of cell migration; IMP:WormBase.
DR GO; GO:0110037; P:regulation of nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR031701; SIX1_SD.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF16878; SIX1_SD; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..335
FT /note="Homeobox protein unc-39"
FT /id="PRO_0000446110"
FT DNA_BIND 225..277
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 27..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 269..294
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..294
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 203
FT /note="R->Q: In e257; uncoordinated locomotion, withered
FT tail morphology, egg-laying defects, protruding vulvae and
FT multiple vulvae. Defective pharyngeal development, which
FT results in animals appearing starved. Mesodermal lineage
FT and neuronal cell migration and specification defects.
FT Abolishes differentiation of RID peptidergic neurons."
FT /evidence="ECO:0000269|PubMed:15282156,
FT ECO:0000269|PubMed:27855782"
FT MUTAGEN 220
FT /note="I->IQF: In ct73; uncoordinated locomotion, withered
FT tail morphology, egg-laying defects, protruding vulvae and
FT multiple vulvae. Defective pharyngeal development, which
FT results in animals appearing starved. Mesodermal lineage
FT and neuronal cell migration and specification defects."
FT /evidence="ECO:0000269|PubMed:15282156,
FT ECO:0000269|PubMed:2361334"
FT MUTAGEN 283
FT /note="P->L: In rh72; uncoordinated locomotion, withered
FT tail morphology, egg-laying defects, protruding vulvae and
FT multiple vulvae. Defective pharyngeal development, which
FT results in animals appearing starved. Mesodermal lineage
FT and neuronal cell migration and specification defects."
FT /evidence="ECO:0000269|PubMed:15282156"
FT MUTAGEN 298
FT /note="P->L: In hp701; abolishes differentiation of RID
FT peptidergic neurons."
FT /evidence="ECO:0000269|PubMed:27855782"
SQ SEQUENCE 335 AA; 38130 MW; C98DC467D75A77DC CRC64;
MTDHPPIDTS SYFDCYQQHQ LPLQYTFTSS SNSNTSNSST SPSHISDQFS SSGGPPYELS
SHILTPSSVI PTPSPSVASA SISSPTIPAF GCTMSEYSME QMEAISTSLF QARDGDRLVA
FFKQLESLYG PNAVDHLRSE AIIVAYTYAL YHSNEFETLF HLLSNRHFQQ RHYNDLQDIW
HHARYKESQL KRGKELNPVE KYRLRRKFPA PKTIWDGEEI VYSFKDSSRK FLKQFFRNVS
EYPTQEQKRE ISRATGLKIV QISNWFKNRR QRDKSNNSAK CSPPSSSSST NGGSDFLPII
TPQSFNLAAA PFNMNMIYGT LRDSQSDNDQ FTFNP
