HM36_CAEEL
ID HM36_CAEEL Reviewed; 257 AA.
AC Q93352;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 02-DEC-2020, sequence version 3.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Homeobox protein ceh-36;
GN Name=ceh-36 {ECO:0000312|WormBase:C37E2.4};
GN ORFNames=C37E2.4 {ECO:0000312|WormBase:C37E2.4};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF 210-GLN--LEU-257.
RX PubMed=14536063; DOI=10.1016/s1534-5807(03)00293-4;
RA Lanjuin A., VanHoven M.K., Bargmann C.I., Thompson J.K., Sengupta P.;
RT "Otx/otd homeobox genes specify distinct sensory neuron identities in C.
RT elegans.";
RL Dev. Cell 5:621-633(2003).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF PRO-80.
RX PubMed=15095973; DOI=10.1016/j.jmb.2003.12.037;
RA Koga M., Ohshima Y.;
RT "The C. elegans ceh-36 gene encodes a putative homeodomain transcription
RT factor involved in chemosensory functions of ASE and AWC neurons.";
RL J. Mol. Biol. 336:579-587(2004).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=21041366; DOI=10.1242/dev.058834;
RA Nakano S., Ellis R.E., Horvitz H.R.;
RT "Otx-dependent expression of proneural bHLH genes establishes a neuronal
RT bilateral asymmetry in C. elegans.";
RL Development 137:4017-4027(2010).
RN [5]
RP FUNCTION, AND INTERACTION WITH SOX-2.
RX PubMed=26341465; DOI=10.15252/embj.201592188;
RA Alqadah A., Hsieh Y.W., Vidal B., Chang C., Hobert O., Chuang C.F.;
RT "Postmitotic diversification of olfactory neuron types is mediated by
RT differential activities of the HMG-box transcription factor SOX-2.";
RL EMBO J. 34:2574-2589(2015).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 66-GLN--LEU-257;
RP 210-GLN--LEU-257 AND PRO-80.
RX PubMed=25738873; DOI=10.1371/journal.pgen.1005003;
RA Walton T., Preston E., Nair G., Zacharias A.L., Raj A., Murray J.I.;
RT "The Bicoid class homeodomain factors ceh-36/OTX and unc-30/PITX cooperate
RT in C. elegans embryonic progenitor cells to regulate robust development.";
RL PLoS Genet. 11:e1005003-e1005003(2015).
CC -!- FUNCTION: Probable transcription factor, acting as a progenitor
CC identity factor regulating the development of lineally-related
CC embryonic cells including glial, excretory and neuronal cells
CC (PubMed:25738873, PubMed:21041366). Mediates chemosensory function of
CC ASE and AWC neurons (PubMed:14536063, PubMed:15095973). In ASE neurons,
CC required to diversify the fate of the ASEL neurons from the ASER
CC neurons (PubMed:14536063, PubMed:15095973). Acts cell-autonomously to
CC establish a neuronal left right asymmetry, possibly promoting
CC asymmetric expression of the helix-loop-helix proteins ngn-1 and hlh-2
CC (PubMed:21041366). In cooperation with the transcription factor sox-2,
CC required for the differentiation of AWC olfactory neurons
CC (PubMed:26341465, PubMed:15095973). May regulate the expression of sox-
CC 2 in AWC olfactory neurons (PubMed:26341465).
CC {ECO:0000269|PubMed:14536063, ECO:0000269|PubMed:15095973,
CC ECO:0000269|PubMed:21041366, ECO:0000269|PubMed:25738873,
CC ECO:0000269|PubMed:26341465}.
CC -!- SUBUNIT: Interacts with sox-2. {ECO:0000269|PubMed:26341465}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in ASE and AWC chemosensory neurons
CC (PubMed:14536063, PubMed:15095973). Expressed left-right asymmetrically
CC in the embryo, in the grandmother cell and the mother cell to the MI
CC pharyngeal motorneuron but in neither analogous precursors of the e3D
CC neuron (PubMed:21041366). {ECO:0000269|PubMed:14536063,
CC ECO:0000269|PubMed:15095973, ECO:0000269|PubMed:21041366}.
CC -!- DEVELOPMENTAL STAGE: Up to the comma stage, expressed in six lineages
CC that produce diverse cell types including pharyngeal cells, muscles,
CC neurons and glia. {ECO:0000269|PubMed:25738873}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR EMBL; BX284606; CAB02821.1; -; Genomic_DNA.
DR PIR; T19809; T19809.
DR RefSeq; NP_510365.1; NM_077964.3.
DR AlphaFoldDB; Q93352; -.
DR SMR; Q93352; -.
DR BioGRID; 46428; 3.
DR IntAct; Q93352; 3.
DR STRING; 6239.C37E2.4; -.
DR EPD; Q93352; -.
DR PaxDb; Q93352; -.
DR EnsemblMetazoa; C37E2.4.1; C37E2.4.1; WBGene00000457.
DR GeneID; 181529; -.
DR KEGG; cel:CELE_C37E2.4; -.
DR UCSC; C37E2.4; c. elegans.
DR CTD; 181529; -.
DR WormBase; C37E2.4; CE08623; WBGene00000457; ceh-36.
DR eggNOG; KOG2251; Eukaryota.
DR GeneTree; ENSGT00940000167436; -.
DR HOGENOM; CLU_991234_0_0_1; -.
DR InParanoid; Q93352; -.
DR OMA; VQVITVW; -.
DR OrthoDB; 1826047at2759; -.
DR SignaLink; Q93352; -.
DR PRO; PR:Q93352; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00000457; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR GO; GO:0048665; P:neuron fate specification; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Chemotaxis; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Nucleus; Reference proteome.
FT CHAIN 1..257
FT /note="Homeobox protein ceh-36"
FT /id="PRO_0000049001"
FT DNA_BIND 55..117
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 33..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 66..257
FT /note="Missing: In ky646; many cells in embryos have
FT defects in both cell cycle timing and cell position, or
FT fail to undergo programmed cell death when expected. About
FT 10% lethality of embryos and 59% reach larval stage 4 (L4)
FT by 6 days."
FT /evidence="ECO:0000269|PubMed:25738873"
FT MUTAGEN 80
FT /note="P->S: In ks86; defective chemotaxis response to
FT sodium acetate and reduced expression of tax-2. About 55%
FT of animals arrest as larvae."
FT /evidence="ECO:0000269|PubMed:15095973,
FT ECO:0000269|PubMed:25738873"
FT MUTAGEN 210..257
FT /note="Missing: In ky640; about 1% lethality of embryos and
FT 78% reach larval stage 4 (L4) by 6 days. Expression of the
FT transcription factor mls-2 is abolished after the L1 stage
FT in the AWC neurons."
FT /evidence="ECO:0000269|PubMed:14536063,
FT ECO:0000269|PubMed:25738873"
SQ SEQUENCE 257 AA; 28581 MW; 36D78E653B21083C CRC64;
MTTNFYTAFP TLGYTAYPQF AFAAATTAPS LTSATTSSTT MAPMAPNSES GRRAGRRERT
SFNRGQLDQL EKVFRETQYP DVHRREALAK AINLPDGRVQ VITVWFKNRR AKDRNNKKMD
GVHPGSTSSR SSNGSPHNES KPDTKSLGIH IPGTPEFNAH SAAKYEANSA VLSQLQQQQQ
QQLQQPKSEL EDSKPLADTK YDSTQSLLPQ AQAAAYASYA TTAPYPYNYN SYFPSNFYYQ
QYGSNDYTPN ITAYSPL
